GEO DataSets

(Submitter supplied) We compared repeatability and comparability of microRNA microarray using 5 different platforms. and compared microarray data generated from five different microRNA microarray platforms with quantitative RT-PCR. Our data suggested that the most accurate and repeatable methods for microRNA expression profiling are Agilent and Toray, and the numbers of detected microRNA at Toray are more than at Agilent. more...

Organism:

Mus musculus; Rattus norvegicus; Homo sapiens

Type:

Non-coding RNA profiling by array

5 related Platforms

30 Samples

Download data: GPR, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens; synthetic construct

Type:

Expression profiling by array; Non-coding RNA profiling by array

5 related Platforms

90 Samples

Download data: GPR, TXT

(Submitter supplied) Microarray technology applied to miRNA profiling is a promising tool in many research fields. At present, only few studies have addressed intra- and inter-platform reproducibility by using tissues/cell-lines of different origin to maximize differences in miRNA content. Although the approach might provide an useful system to address technical issues since, for sure, a huge modulation can be evaluated, it does not reflect the common experimental practice in laboratory. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

18 Samples

Download data: TXT

(Submitter supplied) Microarray technology applied to miRNA profiling is a promising tool in many research fields. At present, only few studies have addressed intra- and inter-platform reproducibility by using tissues/cell-lines of different origin to maximize differences in miRNA content. Although the approach might provide an useful system to address technical issues since, for sure, a huge modulation can be evaluated, it does not reflect the common experimental practice in laboratory. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL8179

18 Samples

Download data: TXT

(Submitter supplied) Microarray technology applied to miRNA profiling is a promising tool in many research fields. At present, only few studies have addressed intra- and inter-platform reproducibility by using tissues/cell-lines of different origin to maximize differences in miRNA content. Although the approach might provide an useful system to address technical issues since, for sure, a huge modulation can be evaluated, it does not reflect the common experimental practice in laboratory. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL14767

18 Samples

Download data: TXT

(Submitter supplied) Microarray technology applied to miRNA profiling is a promising tool in many research fields. At present, only few studies have addressed intra- and inter-platform reproducibility by using tissues/cell-lines of different origin to maximize differences in miRNA content. Although the approach might provide an useful system to address technical issues since, for sure, a huge modulation can be evaluated, it does not reflect the common experimental practice in laboratory. more...

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL14766

18 Samples

Download data: GPR, TXT

(Submitter supplied) Microarray technology applied to miRNA profiling is a promising tool in many research fields. At present, only few studies have addressed intra- and inter-platform reproducibility by using tissues/cell-lines of different origin to maximize differences in miRNA content. Although the approach might provide an useful system to address technical issues since, for sure, a huge modulation can be evaluated, it does not reflect the common experimental practice in laboratory. more...

Organism:

synthetic construct; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL14765

18 Samples

Download data: GPR, TXT

(Submitter supplied) Two reference pools comprised of different miRNA content were hybridized to four different miRNA microarray platforms (Agilent, LC Sciences, Exiqon and a homemade chip (generated from an Inviteogen Ncode probeset)) This dataset includes the reference samples on the Agilent miRNA array. Keywords: miRNA

Organism:

Caenorhabditis elegans; Danio rerio; Drosophila melanogaster; Rattus norvegicus; Murid gammaherpesvirus 4; Betapolyomavirus hominis; Mus musculus; Murid betaherpesvirus 1; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Homo sapiens; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Betapolyomavirus macacae

Type:

Non-coding RNA profiling by array

4 related Platforms

24 Samples

Download data: TXT, XLS

(Submitter supplied) Several technical parameters are now being optimized for microRNA expression profiling experiments, but the finite amount of total RNA available continues to bottleneck clinical investigations. We used microarrays to determine the influence of the labeling reagent on total RNA requirements and data quality.

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL8786

44 Samples

Download data: CEL

(Submitter supplied) This series represents a set of experiments preformed using RNA isolated from human mammary epithelial cells, human MDA-MB-436 cells and HCC1954 cells. Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS817 GDS820 GDS823 GDS825

4 related Platforms

22 Samples

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Expression profiling of breast cancer cell lines HCC 1954 and MDA-MB-436 in reference to mammary epithelial cells. Results used to validate a method of comparing data generated from cDNA arrays to that from Affymetrix arrays. The method uses DNA sequence to match probes between platforms.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 2 cell line sets

Platform:

GPL875

Series:

GSE1299

4 Samples

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Expression profiling of breast cancer cell lines HCC 1954 and MDA-MB-436 in reference to mammary epithelial cells. Results used to validate a method of comparing data generated from Affymetrix arrays to that from cDNA arrays. The method uses DNA sequence to match probes between platforms.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 cell line, 2 disease state sets

Platform:

GPL97

Series:

GSE1299

6 Samples

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Expression profiling of breast cancer cell lines HCC 1954 and MDA-MB-436 in reference to mammary epithelial cells. Results used to validate a method of comparing data generated from Affymetrix arrays to that from cDNA arrays. The method uses DNA sequence to match probes between platforms.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 cell line, 2 disease state sets

Platform:

GPL96

Series:

GSE1299

6 Samples

Download data

Expression profiling of breast cancer cell lines HCC 1954 and MDA-MB-436 in reference to mammary epithelial cells. Results used to validate a method of comparing data generated from Affymetrix arrays to that from cDNA arrays. The method uses DNA sequence to match probes between platforms.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 cell line, 2 disease state sets

Platform:

GPL8300

Series:

GSE1299

6 Samples

Download data

(Submitter supplied) Microarray technology has had a profound impact on gene expression research. Some studies have questioned whether similar expression results are obtained when the same RNA samples are analyzed on different platforms. The MicroArray Quality Control (MAQC) project was initiated to address these concerns, as well as other performance and analysis issues. We demonstrate the consistency of results within a platform across test sites as well as the high level of cross-platform concordance in terms of genes identified as differentially expressed. more...

Organism:

Homo sapiens; Rattus norvegicus

Type:

Expression profiling by array; Expression profiling by RT-PCR

19 related Platforms

943 Samples

Download data: CEL, CSV, GPR, LSR, PDF, TXT, ZIP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6097 GPL570

20 Samples

Download data: CEL, TXT

(Submitter supplied) The present study was conducted to compare the ability of Affymetrix and Illumina microarray technologies to characterize the differential gene expression profiles of human monocytes and monocyte-derived-macrophages. Keywords: Type Cross-platform comparison Summary

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3554

Platform:

GPL570

10 Samples

Download data: CEL

(Submitter supplied) In this study we analyzed the gene expression profiles of human monocytes and monocyte-dervied-macrophages. Keywords: Gene expression profiling

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3553

Platform:

GPL1946

96 Samples

Download data: GPR

(Submitter supplied) The present study was conducted to compare the ability of Affymetrix and Illumina microarray technologies to characterize the differential gene expression profiles of human monocytes and monocyte-derived-macrophages. Keywords: Cross-platform comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3555

Platform:

GPL6097

10 Samples

Download data: TXT

Comparison of monocytes and macrophages. Samples obtained from patients with acute coronary syndrome. Results compared to the performance of Affymetrix arrays (GDS3554). The result from two-color arrays (GDS3553) was used as a reference for the Illumina and Affymetrix arrays.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type, 5 individual sets

Platform:

GPL6097

Series:

GSE10213

10 Samples

Download data: TXT