GEO DataSets

(Submitter supplied) Establishment of pregnancy in ruminants requires blastocyst growth to form an elongated conceptus that produces interferon tau, the pregnancy recognition signal, and initiates implantation. Blastocyst growth and development requires secretions from the uterine endometrium. An early increase in circulating concentrations of progesterone (P4) stimulates blastocyst growth and elongation in ruminants. This study utilized sheep as a model to identify candidate genes and regulatory networks in the endometrium that govern pre-implantation blastocyst growth and development. more...

Organism:

Bos taurus; Ovis aries

Type:

Expression profiling by array

Platform:

GPL8813

10 Samples

Download data: GPR

(Submitter supplied) In order to systematically analyze the maternal, i.e. the endometrial, changes in the equine endometrium underlying the complex embryo-maternal dialogue during early pregnancy, a transcriptome study of endometrium samples from five mares at day 8 and six mares at day 12 of early pregnancy and the corresponding non-pregnant stage was performed.

Organism:

Equus caballus

Type:

Expression profiling by array

Platform:

GPL10248

22 Samples

Download data: TXT

(Submitter supplied) An increase in circulating progesterone (P4) concentrations is associated with increased pregnancy success in beef and dairy cattle. Our objective was to ascertain differential effects of elevated P4 concentrations following conception on endometrial gene expression in beef heifers on Days 5, 7, 13 and 16 of pregnancy, corresponding to the morula, blastocyst, elongation and maternal recognition of pregnancy stages, respectively. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

79 Samples

Download data: CEL

(Submitter supplied) Temporal changes in the embryo transcriptome between the blastocyst stage (Day 7) and initiation of elongation (Day 13) differ between in vivo- and in vitro-derived embryos and are reflective of subsequent developmental fate. The aim of this study was to examine the temporal changes in transcriptional profile as the embryo develops from a spherical blastocyst on Day 7 to an ovoid conceptus at the initiation of elongation on Day 13 and to highlight differences in these temporal gene expression dynamics between in vivo- and in vitro-derived blastocysts which may be associated with embryonic survival/mortality using the bovine Affymetrix microarray.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

12 Samples

Download data: CEL

(Submitter supplied) To get new insights into molecular mechanisms underlying the embryo-maternal communication during the pre-implantation period endometrium samples from Day 18 pregnant vs. non-pregnant twin cows were analyzed using a combination of subtracted cDNA libraries and cDNA array hybridization. Keywords: Comparison of endometrium of pregnant versus control animals

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL3005

10 Samples

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(Submitter supplied) Pretreatment of Animals and Collection of Endometrial Tissue Samples for the production of subtracted cDNA libraries: The estrous cycle of five monozygotic twin pairs (Simmental cows, non-lactating), generated by embryo splitting, which had given birth to an average of one or two calves previously, was synchronized by a single intramuscular injection of 500 µg Cloprostenol (Estrumate®; Essex Tierarznei, München, Germany) at diestrus. more...

Organism:

Bos taurus

1 Series

10 Samples

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(Submitter supplied) The porcine conceptus undergoes rapid differentiation and expansion of its trophoblastic membranes between Days 11 and 12 of gestation. Concomitant with trophoblast elongation, production of conceptus estrogen, the porcine embryonic pregnancy recognition signal increases. Conceptus attachment to the uterine surface epithelium starts after Day 13 initiating epitheliochorial placentation. To analyze the transcriptome changes in the endometrium in the course of maternal recognition of pregnancy (MRP), deep sequencing of endometrial RNA samples of Day 12 pregnant animals (n=4) and corresponding non-pregnant controls (n=4) was performed using Illumina RNA-Seq. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15064

8 Samples

Download data: GTF, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL15670 GPL18884

6 Samples

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(Submitter supplied) PPARG ChIP seq analysis was conducted to determine genes bound by and potentially regulated by PPARG in the developing ovine conceptus. Determination of gene regulation by prostaglandins through PPARG helps to improve our understanding of early pregnancy events and provides a basis for strategies to improve fertility and reproductive efficiency in ruminants.

Organism:

Ovis aries

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15670

2 Samples

Download data: XLSX

(Submitter supplied) RNA seq analysis was conducted to determine gene expression in the day 14 ovine conceptus. This was used in conjunction with the day 14 PPARG ChIP-seq analysis to identify genes bound by PPARG which were also expressed or not expressed in the day 14 conceptus. Understanding changes in gene expression during early pregnancy is critical to improving fertility and reproductive efficiency in ruminants.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18884

4 Samples

Download data: XLSX

(Submitter supplied) In an attempt to unveil part of the molecular processes controlling porcine placentation we have investigated the pregnancy induced gene expression in the porcine endometrium at Day 14 after insemination using the Affymetrix GeneChip® Porcine Genome Array.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

12 Samples

Download data: CEL

(Submitter supplied) In pigs, conceptus attachment to the uterine surface epithelium starts around day 14 of pregnancy preceded by a pronounced vascularization at the implantation zones, initiating the epitheliochorial placentation. To characterize the complex transcriptome changes in the endometrium in the course of initial conceptus attachment deep sequencing of endometrial RNA samples of pregnant animals (n=4) and corresponding cyclic controls (n=4) was performed using Illumina RNA-Seq. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15064

8 Samples

Download data: GTF, TXT

(Submitter supplied) At implantation the endometrium undergoes dramatic modifications necessary for its physical interactions with the trophoblast as well as the development of the conceptus. We aim to identify endometrial factors and pathways essential for a successful implantation in the caruncular [C] and the intercaruncular [IC] areas in cattle. The [C versus IC] direct comparison revealed 1177 and 453 DEG in cyclic and pregnant animals respectively, with a major impact of the conceptus on metabolism and cell adhesion. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2853

20 Samples

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(Submitter supplied) At implantation the endometrium undergoes dramatic modifications necessary for its physical interactions with the trophoblast as well as the development of the conceptus. We aim to identify endometrial factors and pathways essential for a successful implantation in the caruncular [C] and the intercaruncular [IC] areas in cattle. Using a bovine oligo-array, expression profiles were established at day 20 of the estrous cycle or pregnancy (implantation) showing 446 and 1295 differentially expressed genes (DEG) in [C] and [IC] areas respectively. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2853

36 Samples

Download data

(Submitter supplied) Recently, microRNAs (miRNAs), known to modulate gene expression through post-transcriptional mechanisms, were implied in regulation of early pregnancy events including maternal recognition of pregnancy and implantation. To characterize complex transcriptomic changes in expression of miRNAs in pregnant and cyclic endometria collected on days 12, 16 and 20 was analyzed using Illumina deep sequencing. This research showed repertoire of pregnancy-related miRNAs in porcine endometrium during initial stages of conceptus implantation and during the estrous cycle and sheds light on miRNA-mediated gene expression regulation mechanism at the maternal-conceptus interface.

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15064

30 Samples

Download data: TXT, XLSX

(Submitter supplied) To identify genes differentially expressed in the glandless uterus, whole uteri were collected from control (uterine glands present) and PUGKO (no uterine glands) mice at day of pseudopregnancy (DOPP) 3.5 (day DOPP 0.5= vaginal plug). Microarray analysis identified differentially expressed genes in the glandless uteri of PUGKO mice as compared to control mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

8 Samples

Download data: CEL

(Submitter supplied) Laser capture microdissection coupled with microarray genes expression analysis were utilized in order to elucidate the regulatory networks active in epithelial cells of the neonatal and adult mouse uterus.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

8 Samples

Download data: CEL

(Submitter supplied) Delayed implantation occurs in many mammals, but the underlying mechanisms that direct this process remain largely unknown. In mice, ovariectomy prior to the preimplantation ovarian estrogen secretion on day 4 of pregnancy initiates blastocyst dormancy, which can be maintained for more than 200 days by continued progesterone Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS752

Platform:

GPL870

6 Samples

Download data: TIFF, TXT

Comparison of gene expression in dormant and active blastocysts. Dormancy induced by ovariectomy and maintained by progesterone injections. Dormant blastocysts activated by estradiol-17 beta injections. Results provide insight into delayed implantation.

Organism:

Mus musculus

Type:

Expression profiling by array, log ratio, 2 development stage sets

Platform:

GPL870

Series:

GSE1634

6 Samples

Download data: TIFF, TXT

(Submitter supplied) this study was designed to analyze global gene expression in porcine embryos during the preimplantation period (Day 8, 10, and 12 of pregnancy) with the aims of (i) characterizing the dynamics of gene expression profiles and the involved functions and thereby (ii) identifying the chromosome X inactivation profile between female and male

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19176

30 Samples

Download data: TXT, XLSX