GEO DataSets

(Submitter supplied) Juvenile zebrafish were fed Biodiet starter (4% body weight per day) for 42 d with TCDD added at 0 ppb, 0.1 ppb, 1 ppb, 10 ppb or 100 ppb. Fish were collected, sexed, weighed and length measured at 0, 7, 14, 28 or 42 d for TCDD assessment, histopathologic and microarray analysis. Microarray experiments were conducted in TCDD-treated (at 0, 0.1, 1, 10 and 100 ppb) for male and female sexed zebrafish at 28 d. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL17210

36 Samples

Download data: CALLS, PAIR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL17210

81 Samples

Download data: CALLS, PAIR

(Submitter supplied) Juvenile zebrafish were fed Biodiet starter (4% body weight per day) for 42 d with TCDD added at 0 ppb, 0.1 ppb, 1 ppb, 10 ppb or 100 ppb. Fish were collected, sexed, weighed and length measured at 0, 7, 14, 28 or 42 d for TCDD assessment, histopathologic and microarray analysis. Microarray experiments were conducted using 0 and 100 ppb-TCDD treated male and female sexed zebrafish at 7, 14, 28 and 42 d. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL17210

63 Samples

Download data: CALLS, PAIR

(Submitter supplied) 2,3,7,8-TCDD (TCDD) is a reproductive toxicant and endocrine disruptor in nearly all vertebrates, yet the mechanisms by which TCDD induces these reproductive alterations have not been fully characterized. Fish are among the most sensitive vertebrates to the toxic effects of TCDD, and even subtle physiologic changes induced by TCDD can impair reproduction. Previously, we have shown that chronic, sub-lethal exposure to TCDD decreased reproductive capacity, reduced serum estradiol and vitellogenin concentrations, and altered follicular development. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Datasets:

GDS2927 GDS2928

Platform:

GPL1319

8 Samples

Download data: CEL, EXP

Analysis of ovaries following exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations of up to 100 parts per billion (ppb). TCDD is a reproductive toxicant and endocrine disruptor in vertebrates. Results provide insight into molecular mechanisms underlying TCDD toxicity.

Organism:

Danio rerio

Type:

Expression profiling by array, count, 4 dose sets

Platform:

GPL1319

Series:

GSE4859

4 Samples

Download data: CEL, EXP

Analysis of ovaries following exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations of up to 100 parts per billion (ppb). TCDD is a reproductive toxicant and endocrine disruptor in vertebrates. Results provide insight into molecular mechanisms underlying TCDD toxicity.

Organism:

Danio rerio

Type:

Expression profiling by array, count, 4 dose sets

Platform:

GPL1319

Series:

GSE4859

4 Samples

Download data: CEL, EXP

(Submitter supplied) To fully understand molecular toxicity of TCDD in an in vivo animal model, adult zebrafish were exposed to TCDD at 10 nM for 96 h and the livers were sampled for RNA-sequencing based transcriptomic profiling. A total of 1,058 differently expressed genes were identified based on fold-change>2 and TPM (transcripts per million) >10. Among the top 20 up-regulated genes, 10 novel responsive genes were identified and verified by qRT-PCR analysis on independent samples. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10658

2 Samples

Download data: TXT

(Submitter supplied) Retinoic acid (RA) and 2,3,7,8-tetrachlorodibenzo-p-dioxin activate distinct ligand-dependent transcription factors, and both cause cardiac malformation and heart failure in zebrafish embryos. We hypothesized that they cause this response by hyperactivating a common set of genes critical for heart development. To test this, we used microarrays to measure transcripts changes in hearts isolated from zebrafish embryos 1,2,4 and 12 h after exposure to 1μM RA. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Dataset:

GDS2965

Platform:

GPL1319

48 Samples

Download data: CEL, EXP

Analysis of hearts from embryos treated for up to 12 hours with retinoic acid (RA) or 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD). Zebrafish embryos treated with RA or TCDD develop cardiac malformation and heart failure.

Organism:

Danio rerio

Type:

Expression profiling by array, transformed count, 4 agent, 4 time sets

Platform:

GPL1319

Series:

GSE9020

48 Samples

Download data: CEL, EXP

(Submitter supplied) Pregnant C57Bl6N mice were treated with 0 (corn oil), 1.5, 3.0, or 6.0 ug/kg TCDD on gd14.5. Fetal hearts were collected on gd17.5. Hearts from each litter were pooled onto one chip. 4 replicates of each condition were run on affymetrix MG_U74Av2 chips, using standard affymetrix protocols and controls. Keywords: dose-response, 3 doses plus corn oil control, 4 replicates

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1302

Platform:

GPL81

20 Samples

Download data: CEL

Analysis of gestational day (GD) 17.5 fetal hearts isolated from pregnant C57BL/6Ns treated at GD 14.5 with up to 6.0 ug/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). TCDD, a cardiovascular teratogen, inhibits cardiomyocyte proliferation, a process that peaks between GD 14.5 to 17.5.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 4 dose sets

Platform:

GPL81

Series:

GSE2812

20 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Betapolyomavirus macacae; Mus musculus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Danio rerio; Homo sapiens; Rattus norvegicus; JC polyomavirus; Betapolyomavirus hominis

Type:

Non-coding RNA profiling by array; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL15720 GPL10658 GPL7724

36 Samples

Download data: TXT

(Submitter supplied) Although many drugs and environmental chemicals are teratogenic, the mechanisms by which most toxicants disrupt embryonic development are not well understood. microRNAs (miRNAs), single-stranded RNA molecules of ~22 nt that regulate protein expression by inhibiting mRNA translation and promoting mRNA sequestration or degradation, are important regulators of a variety of cellular processes including embryonic development and cellular differentiation. more...

Organism:

Danio rerio

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL10658

12 Samples

Download data: TXT

(Submitter supplied) Although many drugs and environmental chemicals are teratogenic, the mechanisms by which most toxicants disrupt embryonic development are not well understood. microRNAs (miRNAs), single-stranded RNA molecules of ~22 nt that regulate protein expression by inhibiting mRNA translation and promoting mRNA sequestration or degradation, are important regulators of a variety of cellular processes including embryonic development and cellular differentiation. more...

Organism:

Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Betapolyomavirus macacae; Mus musculus; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Danio rerio; Homo sapiens; Rattus norvegicus; Betapolyomavirus hominis

Type:

Non-coding RNA profiling by array

Platforms:

GPL15720 GPL7724

24 Samples

Download data: TXT

(Submitter supplied) The purpose of this experiment is to understand which transcripts are differentially expressed following exposure to TCDD. We used microarrays to understand global changes in gene signal intensity

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL1319

12 Samples

Download data: CEL, XLS

(Submitter supplied) Exposure to environmental contaminants can disrupt normal development of the early vertebrate skeleton. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) impairs craniofacial skeletal development across many vertebrate species and its effects are especially prominent in early life stages of fish. TCDD activates the aryl hydrocarbon receptor (AHR), a transcription factor that mediates most if not all TCDD responses. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL1319

24 Samples

Download data: CEL

(Submitter supplied) 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has a large number of biological effects, including skin, cardiovascular, neurologic disease, diabetes, infertility and cancer. We analysed the in vitro TCDD effects on human CD34+ cells and tested the gene expression modulation by means of microarray analyses before and after TCDD exposure. We identified 253 differentially modulated probe sets, identifying 217 well-characterized genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3889

Platform:

GPL96

8 Samples

Download data: CEL

Analysis of hematopoietic progenitor CD34+ cells from 5 healthy donors (3 paired samples) before and after in vitro 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure. Results provide insight into the molecular pathways of TCDD-induced hematopoietic progenitor cell impairment in bone marrow.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 5 individual sets

Platform:

GPL96

Series:

GSE24193

8 Samples

Download data: CEL