GEO DataSets

(Submitter supplied) RNA seq analysis was conducted to determine gene expression in the day 14 ovine conceptus. This was used in conjunction with the day 14 PPARG ChIP-seq analysis to identify genes bound by PPARG which were also expressed or not expressed in the day 14 conceptus. Understanding changes in gene expression during early pregnancy is critical to improving fertility and reproductive efficiency in ruminants.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18884

4 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL18884 GPL15670

6 Samples

Download data

(Submitter supplied) PPARG ChIP seq analysis was conducted to determine genes bound by and potentially regulated by PPARG in the developing ovine conceptus. Determination of gene regulation by prostaglandins through PPARG helps to improve our understanding of early pregnancy events and provides a basis for strategies to improve fertility and reproductive efficiency in ruminants.

Organism:

Ovis aries

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15670

2 Samples

Download data: XLSX

(Submitter supplied) RNA seq analysis of laser capture microdissected luminal and glandular epithelium from ewes on day of pregnancy 10, 12, 14, 16 and 20. As well as RNA seq of whole conceptuses, and trophectoderm tissue from day 12, 14, 16 and 20 of pregnancy. Determination of gene expression changes in the uterine epithelium and conceptus during early pregnancy helps to improve our understanding of early pregnancy events and provides a basis of new strategies to improve fertility and reproductive efficiency in ruminants.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18884 GPL19778

57 Samples

Download data: XLSX

(Submitter supplied) Establishment of pregnancy in ruminants requires blastocyst growth to form an elongated conceptus that produces interferon tau, the pregnancy recognition signal, and initiates implantation. Blastocyst growth and development requires secretions from the uterine endometrium. An early increase in circulating concentrations of progesterone (P4) stimulates blastocyst growth and elongation in ruminants. This study utilized sheep as a model to identify candidate genes and regulatory networks in the endometrium that govern pre-implantation blastocyst growth and development. more...

Organism:

Bos taurus; Ovis aries

Type:

Expression profiling by array

Platform:

GPL8813

10 Samples

Download data: GPR

(Submitter supplied) Exposure of bovine conceptuses to colony stimulating factor 2 (CSF2) from Day 5 to 7 of development can increase the percent of transferred conceptuses that develop to term. The purpose of this experiment was to understand the mechanism by which CSF2 increases embryonic and fetal survival. Conceptuses were produced in vitro in the presence or absence of 10 ng/ml CSF2 from Day 5 to 7 after insemination, transferred into cows, and flushed from the uterus at Day 15 of pregnancy. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11649

16 Samples

Download data

(Submitter supplied) The present studies tested the hypothesis that the elongating ovine conceptus and uterus produces EVs with the potential to mediate conceptus-maternal communication during early pregnancy. In Study One, EVs were purified from uterine luminal fluid (ULF) of day 14 cyclic sheep. The EVs were fluorescently labeled with PKH67 dye and infused into the uterine lumen of pregnant sheep for 6 days using an osmotic pump. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19778

3 Samples

Download data: TXT

(Submitter supplied) Transcriptome profiling of in vivo-derived porcine conceptus at the tubular stage (12-80 mm) during the elongation period of peri-implantation development. Keywords: Embryo stage of development

Organism:

Sus scrofa

Type:

Expression profiling by SAGE

Platform:

GPL1270

1 Sample

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(Submitter supplied) This study relates to pregnancy outcome after assisted reproduction of fertility-classified cattle. The aim is to investigate how the uterine environment impacts and programs conceptus survival and development. The study found that ripple effects of dysregulated conceptus-endometrial interactions elicit post-elongation pregnancy loss in subfertile animals during the implantation period.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23055

52 Samples

Download data: TXT

(Submitter supplied) The hippocampus is especially susceptible to age-associated neuronal pathologies, and there is concern that the age-associated rise in cortisol secretion from the adrenal gland may contribute to their etiology. Furthermore, because 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) catalyzes the reduction of cortisone to the active hormone cortisol, it is plausible that an increase in the expression of this enzyme enhances the deleterious impact of cortisol in the hippocampus and contributes to the neuronal pathologies that underlie cognitive decline in the elderly. more...

Organism:

Macaca mulatta

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27943

28 Samples

Download data: CSV

(Submitter supplied) The liver x receptor (LXR) and peroxisome proliferator-activated receptor gamma (PPARG) nuclear receptor transcription factors (TFs) are master regulators of energy homeostasis that impact tumor cell metabolism and proliferation. To better define the underlying molecular processes governing the genetic control of cellular growth in response to extracellular metabolic signals, we performed a comprehensive, genome-wide characterization of the temporal regulatory cascades mediated by LXR and PPARG signaling in HT29 colorectal cancer cells. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL11154

82 Samples

Download data: BED, TXT

(Submitter supplied) To further characterize the roles of cortisol signaling via the glucocorticoid receptor (GR) in developing zebrafish, we have used morpholino oligonucleotides to knockdown GR protein translation and measured gene expression in RNA extracted from 24 and 36 hours post fertilization (hpf) embryos. The GR morpholino was characterized previously in Nesan et al., 2012, Endocrinology 181, 35-44)

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL7301

12 Samples

Download data: TXT

(Submitter supplied) Exosomes/microvesicles (hereafter referred to as extracellular vesicles) were isolated from the ULF of day 14 cyclic and pregnant ewes using ExoQuick-TC. Extracellular vesicle RNA was pooled (n=4 per status) and analyzed for small RNAs by sequencing on the Ion Torrent PGM platform and analysis with CLC Genomics Workbench small RNA workflow based on the miRBase (Release 19) Bos taurus database.

Organism:

Ovis aries

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18156

2 Samples

Download data: XLS

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL21290 GPL16791

34 Samples

Download data: TXT

(Submitter supplied) RXRA regulates transcription as part of a heterodimer with 14 other nuclear receptors, including the peroxisome proliferator-activated receptors (PPARs). Analysis from the TCGA raised the possibility that hyperactive PPAR signaling, either due to PPAR gamma gene amplification or RXRA hot-spot mutation (S427F/Y) drives 20-25% of bladder cancers. Here we characterize mutant RXRA, demonstrating it induces enhancer/promoter activity in the context of RXRA/PPAR heterodimers. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL16791 GPL21290

18 Samples

Download data: TXT

(Submitter supplied) RXRA regulates transcription as part of a heterodimer with 14 other nuclear receptors, including the peroxisome proliferator-activated receptors (PPARs). Analysis from the TCGA raised the possibility that hyperactive PPAR signaling, either due to PPAR gamma gene amplification or RXRA hot-spot mutation (S427F/Y) drives 20-25% of bladder cancers. Here we characterize mutant RXRA, demonstrating it induces enhancer/promoter activity in the context of RXRA/PPAR heterodimers. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21290

16 Samples

Download data: BROADPEAK, NARROWPEAK

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL21332 GPL9126

8 Samples

Download data: TXT

(Submitter supplied) In this study, we uncover the molecular mechanism that PPARD transactive-deficient pigs have large-eared feature. Evidently from this work, PPARD plays a vital role on inhibiting cartilage growth by regulating the transcription of critical genes for chondrogenesis in ligand dependent manner; the activation of sPPARD speeds up apoptosis in CSPCs, terminal differentiation of chondroblasts and matrix degradation in auricular cartilage. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21332

6 Samples

Download data: TXT, XLS

(Submitter supplied) In this study, we uncover the molecular mechanism that PPARD transactive-deficient pigs have large-eared feature. Evidently from this work, PPARD plays a vital role on inhibiting cartilage growth by regulating the transcription of critical genes for chondrogenesis in ligand dependent manner; the activation of sPPARD speeds up apoptosis in CSPCs, terminal differentiation of chondroblasts and matrix degradation in auricular cartilage. more...

Organism:

Sus scrofa

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9126

2 Samples

Download data: TXT

(Submitter supplied) Background: During the process of elongation, the embryo increases in size within the uterus while the extra-embryonic tissues (EET) develop and differentiate in preparation for implantation. As it grows, the ovoid embryo changes shape into first a tubular and then a filamentous form. This process is directed by numerous genes and pathways, the expression of which may be altered in the case of developmental irregularities such as when the conceptus is shorter than expected or when the embryo develops after splitting. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL7417

24 Samples

Download data: TXT