GEO DataSets

(Submitter supplied) Cells were cotreated with estrogen and dihydrotestosterone, progesterone or medroxyprogesterone acetate to assess the combinatorial effects of progestogens or androgens with estrogen in T47D breast cancer cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6171

24 Samples

Download data: TXT

(Submitter supplied) A timecourse assessment of the response of ZR-75-1 breast cancer cells to progestogens in cells pretreated with estrogen. P4 treatment in cells pretreated with E2 results in a unique transcriptional response, including upregulation of ErbB growth factor pathways and alteration of the intrinsic subtype of the breast cancer cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

44 Samples

Download data: CEL

(Submitter supplied) Cells were cotreated with dihydrotestosterone, progesterone or medroxyprogesterone acetate and estrdiol to assess the combinatorial effects of hormone exposure in breast cancer cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6884

36 Samples

Download data: TXT

(Submitter supplied) Background Estrogen and progesterone are potent breast mitogens. In addition to steroid hormones, multiple signaling pathways input to estrogen receptor (ER) and progesterone receptor (PR) actions via posttranslational events. Protein kinases commonly activated in breast cancers phosphorylate steroid hormone receptors (SRs) and profoundly impact their activities. Methods To better understand the role of modified PRs in breast cancer, we measured total and phospho-Ser294 PRs in 209 human breast tumors represented on 2754 individual tissue spots within a tissue microarray and assayed the regulation of this site in human tumor explants cultured ex vivo. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

84 Samples

Download data: TXT

(Submitter supplied) Medroxyprogesterone acetate (MPA) is a progestin that can bind to and activate progesterone, androgen and glucocorticoid receptors. However, it is not known which receptor mediates MPA action in a cellular context where all three of these receptors are co-expressed and functional. This microarray experiment was performed to compare the transcriptomes induced by MPA and the cognate ligands for these receptors ie progesterone (PROG), 5a-dihydrotestosterone (DHT) and dexamethasone (DEX) in breast cancer cells to determine which was most similar to MPA.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

15 Samples

Download data: CEL

(Submitter supplied) To obtain a global analysis of the effect of TPA on the Progesterone Receptor (PR) cistrome

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL18573

24 Samples

Download data: BIGWIG

(Submitter supplied) We have identified differentially PR-regulated genes in T47D cells and mapped PR-binding sites by using next generation sequencing

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9052

4 Samples

Download data: TAB, TXT

(Submitter supplied) To identify inter-individual differences in transcriptional cell state in the human breast, we performed scRNAseq analysis on 86,136 cells collected from 28 healthy premenopausal donors who underwent reduction mammoplasty surgery. To obtain an unbiased snapshot of the epithelium and stroma, we collected live (DAPI negative) / singlet cells from all samples by fluorescence activated cell sorting (FACS). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20301 GPL24676

22 Samples

Download data: RDS

(Submitter supplied) In order to evaluate the effects of RIZ2 overexpression on gene expression, a pilot expression study was performed through microarrays analysis. We compared the differential gene expression between HEK-293 cells overexpressing human RIZ2 (pEGFP-hRIZ2) and rat RIZ1 (pEGFP-rRIZ1) versus control cells transfected with the E-GFP empty vector alone (pEGFP).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10332

12 Samples

Download data: TXT