GEO DataSets

(Submitter supplied) To determine the biological effects of MPS1 inhibition (both by siRNA and Drug (NMSP715)) on signaling pathways in GBM cells (U251 &U87), we profiled the modulation of phosphorylated and non-phosphorylated proteins using RPPA

Organism:

Homo sapiens

Type:

Protein profiling by protein array

Platform:

GPL19976

24 Samples

Download data: XLSX

(Submitter supplied) Gene expression changes were analyzed in U251 GBM cells after downregulation of MPS1 by RNA interference technology at different time points

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

18 Samples

Download data: CEL

(Submitter supplied) To identify signaling pathways that are differentially regulated in human gliomas, a microarray analysis on 30 brain tumor samples (12 primary glioblastomas (GBM), 3 secondary glioblastomas (GBM-2), 8 astrocytomas (Astro) and 7 oligodendrogliomas (Oligo)) and on 5 glioblastoma cell lines (LN018, LN215, LN229, LN319 and BS149) was performed. Normal brain tissue (NB) and normal human astrocytes (NHA) were used as a control. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS4467 GDS4468

Platform:

GPL570

45 Samples

Download data: CEL

Analysis of 5 glioblastoma cell lines (LN018, LN215, LN229, LN319 and BS149). Results provide insight into molecular mechanisms underlying glioblastoma multiforme and other aggressive brain cancers.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 cell line sets

Platform:

GPL570

Series:

GSE15824

10 Samples

Download data: CEL

Analysis of primary glioblastomas (GBM), astrocytomas, oligodendrogliomas and secondary GBM brain tumors. MNK1 kinase upregulation observed in primary GBM brain tumors. Results identifiy signaling pathways differentially regulated in gliomas.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 cell type, 8 other, 3 tissue sets

Platform:

GPL570

Series:

GSE15824

35 Samples

Download data: CEL

(Submitter supplied) Glioblastoma multiforme (GBM) is the most aggressive form of brain tumors. Despite radical surgery and radiotherapy supported by chemotherapy, the disease still remains incurable with extremely low median survival rate of 12-15 months from the time of initial diagnosis. The main cause of treatment failure is considered to be the presence of cells that are resistant to such treatment. MicroRNAs (miRNAs) as regulators of gene expression are involved in the tumor pathogenesis, including GBM. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

6 Samples

Download data: CEL

(Submitter supplied) Glioblastoma is the most aggressive and lethal malignant brain tumor. miRNA expression profiling could be useful in improving the classification of tumors and predicting their behavior. In this study, the miRNA expression patterns in glioblastoma tumor tissues and adjacent normal tissues were identified through expression profiling of a patient with glioblastoma. The results will hopefully enhance our understandings of the epigentic changes in glioblastoma progression and provide candidates for miRNAs-based targeting tharapy.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18402

2 Samples

Download data: TXT

(Submitter supplied) miR-490 is robustly downregulated in GBM tumour samples. This study identifies the genes differentially expressed upon miR-490 overexpression in U87MG glioblastoma cell line. GeneChip PrimeView Human Gene Expression Array was used to assess mRNA expression profile in response to miR-490 overexpression in U87MG cell line.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15207

4 Samples

Download data: CEL

(Submitter supplied) Glioblastoma (GBM) is classified as World Health Organization grade IV tumors of the central nervous system, and it is the most malignant form of glioma. The current GBM therapies could not completely eliminate the tumor mass and the occurrence of therapeutic resistance of surviving GBM cells is considered as an obstacle to be overcome. We used microarrays to detail the global program of gene expression underlying development of radioresistance of GBM and identified a variety of genes whose expressions were regulated during this process.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

2 Samples

Download data: CEL

(Submitter supplied) In this study, we performed miRNA profiles analysis of 84, 17, 20, 83, 718, 1123, 528 and 816 glioma spheres compared to normal progenitor sample (16wf) using microarray to evaluate their potential role in regulation of the biological properties of proneural and mesenchymal glioma spheres

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL22124

9 Samples

Download data: XLS

(Submitter supplied) Tumor heterogeneity of high-grade glioma (HGG) is recognized by four clinically relevant subtypes based on core gene signatures. However, molecular signaling in glioma stem cells (GSCs) in individual HGG subtypes is poorly characterized. Here we identified and characterized two mutually exclusive GSC subtypes with distinct dysregulated signaling pathways. Analysis of mRNA profiles distinguished proneural (PN) from mesenchymal (Mes) GSCs and revealed a pronounced correlation with the corresponding PN or Mes HGGs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

48 Samples

Download data: CEL

(Submitter supplied) We created a computational framework with which to predict BUB1B-GLEBS sensitivity based on gene expression data from BUB1B-inhibition sensitive (BUB1BS) or resistant (BUB1BR) GBM stem cells (GSCs), astrocytes, and neural progenitors. Our classifier examines the expression of 838 genes comprising the BUB1BS signature. Applying this scheme to GBM patient tumor data stratified tumors into BUB1BS and BUB1BR subtypes, revealing that BUB1BS patients have a significantly worse prognosis regardless of their tumor's development subtype (i.e., classical, mesenchymal, neural, proneural). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

18 Samples

Download data: FPKM_TRACKING

(Submitter supplied) Pooled samples of primary GBM tissues, primary cultures, and normal tissues were analyzed by MPSS.

Organism:

Homo sapiens

Type:

Expression profiling by MPSS

Platform:

GPL8198

2 Samples

Download data: TXT

(Submitter supplied) The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. more...

Organism:

Homo sapiens

1 Series

2 Samples

Download data

(Submitter supplied) Inactivating mutations of genes encoding the cohesin complex are common in a wide range of human cancers. STAG2 is the most commonly mutated subunit. Here we report the impact of stable correction of endogenous, naturally occurring STAG2 mutations on gene expression, 3D genome organization, chromatin loops, and Polycomb signaling in glioblastoma multiforme. Correction of mutant STAG2 significantly altered the expression of ~10% of all expressed genes. more...

Organism:

Homo sapiens

Type:

Other; Expression profiling by high throughput sequencing

Platform:

GPL24676

20 Samples

Download data: TXT, XLSX

(Submitter supplied) MicroRNAs have emerged as major genetic elements in the genesis and suppression of cancer. Here, multi-dimensional cancer genome analysis and validation has defined a novel Glioblastoma Multiforme (GBM) tumor suppressor pathway and mechanism of action centered on Quaking (QK), a member of the STAR family of RNA-binding proteins. Combined functional, biochemical and computational studies establish that p53 directly regulates QK gene expression, QK protein binds and stabilizes miR-20a of the cancer-relevant miR-17-92 cluster, and miR-20a in turn functions to regulate TGFβR2 and the TGFβ signaling network. more...

Organism:

Murid gammaherpesvirus 4; human gammaherpesvirus 4; Betapolyomavirus macacae; Mus musculus; Rattus norvegicus; Murid betaherpesvirus 1; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Homo sapiens; Human alphaherpesvirus 1; Human betaherpesvirus 5; Betapolyomavirus hominis

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL1261 GPL7723 GPL570

48 Samples

Download data: CEL, TXT

(Submitter supplied) To identify QK-modulated microRNAs exhibiting a >1.5-fold change across all three cell model systems: human GBM cell lines, U87 and Hs683, and Ink4a/Arf-/- Pten-/- mouse astrocytes

Organism:

Mus musculus; Human alphaherpesvirus 1; human gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus macacae; Homo sapiens; Human betaherpesvirus 5; Murid gammaherpesvirus 4; Betapolyomavirus hominis; Rattus norvegicus; Murid betaherpesvirus 1; JC polyomavirus; Human immunodeficiency virus 1

Type:

Non-coding RNA profiling by array

Platform:

GPL7723

6 Samples

Download data: TXT

(Submitter supplied) To identify QK-modulated microRNAs exhibiting a >1.5-fold change across all three cell model systems: human GBM cell lines, U87 and Hs683, and Ink4a/Arf-/- Pten-/- mouse astrocytes

Organism:

Mus musculus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; Human immunodeficiency virus 1; human gammaherpesvirus 4; JC polyomavirus; Human gammaherpesvirus 8; Betapolyomavirus macacae; Rattus norvegicus; Homo sapiens; Murid gammaherpesvirus 4; Betapolyomavirus hominis

Type:

Non-coding RNA profiling by array

Platform:

GPL7723

12 Samples

Download data: TXT

(Submitter supplied) Identify potential miR-20a regulated mRNAs and linked pathways in the setting of QK knockdown by comparing the transcriptional profiles of shQK-transduced human U87 cells together with miR-20a or a scrambled miRNA control (miR-NT)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

4 Samples

Download data: CEL

(Submitter supplied) Identify potential miR-20a regulated mRNAs and linked pathways in the setting of QK knockdown by comparing the transcriptional profiles of shQK-transduced primary mouse Ink4a/Arf-/- Pten-/- astrocytes together with miR-20a or a scrambled miRNA control (miR-NT)

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL