GEO DataSets

(Submitter supplied) Alternative polyadenylation (APA) contributes to post-transcriptional regulation, but its role in Alzheimer’s disease (AD) is largely unknown. Using high-resolution SQUARE multiple 3’ primer-based sequencing, we discovered massive APA differences in temporal gyrus tissues from demented AD patients compared to either healthy controls or non-demented donors with AD neuropathology (NDWP). Advanced statistics, microfluidics RT-PCR and protein measurements validated known and novel APA-modified 3’-intact transcripts. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL16791

72 Samples

Download data: TXT

(Submitter supplied) Alternative polyadenylation (APA) contributes to post-transcriptional regulation, but its role in Alzheimer’s disease (AD) is largely unknown. Using high-resolution SQUARE multiple 3’ primer-based sequencing, we discovered massive APA differences in temporal gyrus tissues from demented AD patients compared to either healthy controls or non-demented donors with AD neuropathology (NDWP). Advanced statistics, microfluidics RT-PCR and protein measurements validated known and novel APA-modified 3’-intact transcripts. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16288

24 Samples

Download data: TXT

(Submitter supplied) We used Illumina Human HT-12 v4 arrays to compare RNA expression of middle temporal gyrus (MTG; BA21) in Alzheimer’s Disease (AD = 97) and non-demented controls (ND = 98). A total of 938 transcripts were highly differentially expressed (adj p < 0.01; log2 Fold Change (FC) ≥ |0.500|, with 411 overexpressed and 527 underexpressed in AD. Our results correlated with expression profiling in neurons from AD and ND obtained by Laser Capture Microscopy in MTG from an independent dataset (log2 FC correlation: r = 0.504; p = 2.2e-16). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

195 Samples

Download data: TXT, XLSX

(Submitter supplied) Aging is the primary risk factor of neurodegenerative disorders such as Alzheimer's disease (AD). However, the molecular events occurring during brain aging are extremely complex and still largely unknown. For a better understanding of these age-associated modifications, animal models as close as possible to humans are needed. We thus analyzed the transcriptome of the temporal cortex of the primate Microcebus murinus using human oligonucleotide microarrays (Affymetrix). more...

Organism:

Microcebus murinus; Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4128

Platform:

GPL570

18 Samples

Download data: CEL, CHP

Analysis of temporal cortex of young adult, old healthy, and Alzheimer’s disease (AD-like) animals. AD-like animals presented ß-amyloid plaques and cortical atrophy, which are signs of AD in humans. Results provided insight into molecular basis of physiological versus pathological brain aging.

Organism:

Homo sapiens; Microcebus murinus

Type:

Expression profiling by array, count, 2 age, 2 disease state, 2 gender sets

Platform:

GPL570

Series:

GSE21779

18 Samples

Download data: CEL, CHP

(Submitter supplied) Unravel the mechanisms underlying brain aging and Alzheimer´s disease (AD) has been difficult because of complexity of the networks that drive these aging-related changes. Analysis of the gene expression in the brain is a valuable tool to study the function of the brain under normal and pathological conditions. Gene microarray technology allows massively parallel analysis of most genes expressed in a tissue, and therefore is an important research tool that potentially can provide the investigative power needed to address the complexity of brain aging and neurodegenerative processes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1930

128 Samples

Download data

(Submitter supplied) Human post-mortem brain samples from Alzheimer’s disease (AD) and control individuals were processed for small RNA-sequencing followed by comparative analyses

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11154

10 Samples

Download data: TXT