GEO DataSets

(Submitter supplied) Cultures of Y. pestis Kimberley53 virulent strain were exposed to different concentrations of ciprofloxacin (including 0.016 µg/ml representing the strains' MIC value) for various time periods. Total RNA samples were extructed and used for cRNA synthesis and labelling (using Cy3-CTP for the treated samples and Cy5-CTP for the non-treated sample in one biological experiment and dye swap for the 2nd replicate of independent biological experiment). more...

Organism:

Yersinia pestis

Type:

Expression profiling by array

Platform:

GPL21664

8 Samples

Download data: TXT

(Submitter supplied) Francisella tularensis, the etiological agnet of Tularemia is a Category A select agent. Preparedness means for prompt proper antibiotic treatment are of need to prevent morbidity and mortality. While standard antimicrobial susceptibility tests are time consuming, the quantification of the changes in the expression levlels of specific mRNA markers folowing antibiotic exposure may enable a rapid determination of antimicrobial susceptibility. more...

Organism:

Francisella tularensis subsp. tularensis SCHU S4

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32560

12 Samples

Download data: TXT

(Submitter supplied) Antibiotic resistance (AR) is one of the greatest threats to global health and is associated with higher treatment costs, longer hospital stays, and increased mortality. Current gold standard antibiotic susceptibility tests (AST) are dependent on organism growth rates resulting in prolonged diagnostic answers for slow growing organisms. Changes in the cellular transcriptome can be instantaneous in the presence of stressors such as antibiotic pressure. more...

Organism:

Bacillus anthracis; Yersinia pestis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33703 GPL29781

48 Samples

Download data: XLSX

(Submitter supplied) Yersinia pseudotuberculosis is a Gram-negative bacterium capable of causing gastrointestinal infection and is closely related to the highly virulent plague bacillus Yersinia pestis. Infection by both species are currently treatable with antibiotics such as ciprofloxacin, a quinolone-class drug of major clinical importance in the treatment of many other infections. Our current understanding of the mechanism of action of ciprofloxacin is that it inhibits DNA replication by targeting DNA gyrase, and that resistance is primarily due to mutation at this target site, along with generic efflux and detoxification strategies. more...

Organism:

Yersinia pseudotuberculosis IP 32953

Type:

Other

Platform:

GPL27002

3 Samples

Download data: TXT

(Submitter supplied) Yersinia pestis, the etiologic agent of plague, emerged as a flea-borne pathogen only within the last 6,000 years. Just five simple genetic changes in the Yersinia pseudotuberculosis progenitor, which served to eliminate toxicity to fleas and to enhance survival and biofilm formation in the flea digestive tract, were key to the transition to the arthropod-borne transmission route. To gain a deeper understanding of the genetic basis for the development of a transmissible biofilm infection in the flea foregut, we evaluated additional gene differences and performed in vivo transcriptional profiling of Y. more...

Organism:

Yersinia pestis; Yersinia pestis CO92; Yersinia pseudotuberculosis; Yersinia pestis KIM10+

Type:

Expression profiling by array

Platform:

GPL25510

54 Samples

Download data: CEL, CHP