GEO DataSets

(Submitter supplied) Isolated islets used for transplantation are known to be stressed, which can result from the circumstances of death, in particular brain dead, the preservation of the pancreas with its warm and cold ischemia, from the trauma of the isolation process, and the complex events that occur during culture tissue. Isolated islets used for transplantation are known to be stressed, which can result from the circumstances of death, in particular brain death, the preservation of the pancreas with its warm and cold ischemia, from the trauma of the isolation process, and the complex events that occur during tissue culture. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

14 Samples

Download data: CEL

(Submitter supplied) It is known that the stresses encountered during islet isolation have deleterious effects on beta-cell physiology. The nature of these effects, however, is incompletely known, partly due to the heterogeneity of islet preparations. The objective was to assess the genome-wide effect of islet isolation and in-vitro culture on beta-cell transcriptome. Beta cells identified by their intrinsic autofluorescence, were captured from donor pancreas and isolated islets using Laser Capture Microdissection. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6884

21 Samples

Download data: TXT

(Submitter supplied) Changes in gene expression in pancreatic beta-cells from type 2 diabetes could provide insights into their abnormal insulin secretion and beta-cell turnover. The laser capture microdissection technique was used to acquire beta-cells from pancreatic tissue sections obtained from type 2 diabetic (T2D) and non-diabetic controls. We found that 4% of analyzed transcripts were differentially expressed between the two groups at the lower confidence bound cutoff of 1.2, and, among the differentially expressed transcripts, 62% were up-regulated and 38% down-regulated in samples of T2D subjects compared to non-diabetic controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3782

Platform:

GPL1352

20 Samples

Download data: CEL

Analysis of beta-cell enriched pancreatic tissue obtained by laser capture microdissection from subjects with type 2 diabetes (T2D). In subjects predisposed to T2D, beta-cell dysfunction occurs early in the course of the disease. Results provide further insight into the pathogenesis of T2D.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state sets

Platform:

GPL1352

Series:

GSE20966

20 Samples

Download data: CEL

(Submitter supplied) Islet transplantation exposes beta cells to mild hyperglycemia and to the abnormal environment of the transplant site. These conditions may affect beta cells and induce the expression of genes involved in beta cell damage. Gene expression profile of human beta cells exposed to mild hyperglycemia by transplantation into ICR-SCID mice was evaluated and compared with the gene profile of beta cells obtained from non-diabetic subjects. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

13 Samples

Download data: CEL