GEO DataSets

(Submitter supplied) In the context of lentiviral vector (LV)-mediated gene correction of hematopoietic stem and progenitor cells (HSPC), the viral origin of LV could trigger cellular responses with potential functional consequences that have not been investigated to date. Here, a time-course genome-wide transcriptional profiling of transduced human HSPC reveals a remarkably limited impact of LV on cellular gene expression, supporting some stealth features of the vector. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

93 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Other

Platforms:

GPL24676 GPL18573 GPL15520

612 Samples

Download data: TXT

(Submitter supplied) Long-range gene editing by homology-directed repair (HDR) in hematopoietic stem/progenitor cells (HSPCs) often relies on viral transduction with recombinant adeno-associated viral vector (AAV) for template delivery. Here, we uncover unexpected load and prolonged persistence of AAV genomes and their fragments, which trigger sustained p53-mediated DNA damage response (DDR) upon recruiting the MRE11-RAD50-NBS1 (MRN) complex on the AAV inverted terminal repeats (ITRs). more...

Organism:

Homo sapiens

Type:

Other

Platforms:

GPL24676 GPL18573

429 Samples

Download data: TSV

(Submitter supplied) Long-range gene editing by homology-directed repair (HDR) in hematopoietic stem/progenitor cells (HSPCs) often relies on viral transduction with recombinant adeno-associated viral vector (AAV) for template delivery. Here, we uncover unexpected load and prolonged persistence of AAV genomes and their fragments, which trigger sustained p53-mediated DNA damage response (DDR) upon recruiting the MRE11-RAD50-NBS1 (MRN) complex on the AAV inverted terminal repeats (ITRs). more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL15520

183 Samples

Download data: TXT

(Submitter supplied) With the goal of specifically dissecting the toxicogenomic signatures of the helper-dependent (HD) human (HAd5) and canine (CAV-2) adenovirus, the VSV-G-pseudotyped SIN HIV-1 (LV) and the Adenoviral-associated vector 2/9 for human neurons (AAV2/9), we transduced a bona fide human neuronal system with HD-HAd5, HD-CAV-2, LV and AAV2/9, we analysed the transcriptional response of more than 47,000 transcripts using gene chips. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4861

Platform:

GPL570

30 Samples

Download data: CEL

Temporal analysis of differentiated mNPC cultures transduced with three vectors: Helper-dependent (HD) human (HAd) and canine (CAV-2) adenoviruses and VSV-G-pseudotyped self-inactivating HIV-1 vector (LV). Results provide insight into the toxicogenomic profiles of these viral vectors in mNPCs.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 5 agent, 2 time sets

Platform:

GPL570

Series:

GSE47130

30 Samples

Download data: CEL