GEO DataSets

(Submitter supplied) Human NK cells activity against cancer cells is deeply suppressed by TGF-β1, an immunomodulatory cytokine that is released and activated in the tumor microenvironment. Moreover, our previous data showed that TGF-β1 modifies the chemokine receptor repertoire of NK cells. In particular, it decreases the expression of CX3CR1 that drives these effectors toward peripheral tissues, including tumor sites. In order to identify possible mechanisms mediating chemokine receptors modulation, we analyzed the miRNA profile of TGF-β1-treated primary NK cells. The analysis pointed out miR-27a-5p as a possible modulator of CX3CR1. We demonstrated the functional interaction of miR-27a-5p with the 3’ untranslated region (3’UTR) of CX3CR1 mRNA by two different experimental approaches: by the use of a luciferase assay based on a reporter construct containing the CX3CR1 3’UTR and by transfection of primary NK cells with a miR-27a-5p inhibitor. We also showed that the TGF-β1-mediated increase of miR-27a-5p expression is a consequence of miR-23a-27a-24-2 cluster induction. Moreover, we demonstrated that miR-27a-5p down-regulates the surface expression of CX3CR1. Finally we showed that Neuroblastoma cells induced in resting NK cells a downregulation of the CX3CR1 expression that was paralleled by a significant increase of miR-27a-5p expression. Therefore, the present study highlights miR-27a-5p as a pivotal TGF-β1-induced regulator of CX3CR1 expression.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL18942

9 Samples

Download data: TXT

(Submitter supplied) To investigate the role of TGF-β1-regulated miRNAs in the progression of RMS,we performed comprehensive miRMA microarray analysis on RNA derived from typical RMS cell lines and TGF-β1 knock-down cell lines. We identified a novel set of TGF-β1-related miRNAs.

Organism:

Homo sapiens; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Betapolyomavirus hominis; Mus musculus; Rattus norvegicus; Murid betaherpesvirus 1; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus macacae; Human alphaherpesvirus 2; Murid gammaherpesvirus 4

Type:

Non-coding RNA profiling by array

Platform:

GPL11434

6 Samples

Download data: TXT

(Submitter supplied) We examined miRNA profiles of human NK cells from different cell compartments (peripheral blood, cord blood, and uterine deciduas) and of NKT and T cells from peripheral blood, and identified distinct classes of up-regulated microRNAs in different human NK cells.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL10850

5 Samples

Download data: TXT

(Submitter supplied) To identify miRNAs involved in ST-induced cell transformation, we used microarray chips containing 856 miRNA probes to define miRNA expression profiles in 16HBE,16HBER and 16HBERST cells.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL11332

2 Samples

Download data: XLS

(Submitter supplied) Nasal NK-cell lymphomas are EBV-infected lymphomas of the nasal mucosa. The genome-wide miRNA expression profiles of 36 cases of NNLs are determined with the NanoString technology. It is found that the expression levels of let-7 inhibits progression of nasal lymphomas.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18086

36 Samples

Download data: RCC

(Submitter supplied) YT and NK92 cells are EBV-infected lymphoma cells of NK- cell origin. YT cells have weaker expression of T-bet and IFNg than NK92 cells. However, this data set shows that both cell lines have similar mRNA levels, implying post-transcriptional regulations.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL

(Submitter supplied) The goal of this study is to find out the exact mechanisms of action of silymarin (SM), a complex mixture of flavonolignans structures isolated from milk thistle (Silybum marianum), on the biological behavior (phenotype) of human HaCaT keratinocytes.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

6 Samples

Download data: TXT