GEO DataSets

(Submitter supplied) The present study concerns the effects of 21 days of sustained recumbence (bedrest) on skeletal muscle gene expression. 14 male subjects participated in normoxic bedrest (NBR) with muscle unloading, for 21 days, this is a substudy of tha PlanHab-study which apart from normoxic bedrest also involved hyboxic bedrest and hypoxic ambulation Throughout both bedrest interventions, each subject remained in a horizontal position at all times. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

24 Samples

Download data: CEL

(Submitter supplied) The present study concerns the effects of 90 days of sustained recumbence (bedrest) on skeletal muscle gene expression with and without exercise countermeasures.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

41 Samples

Download data: CEL

(Submitter supplied) Short-term bed rest is used to simulate muscle disuse in humans. In our previous reports, we found that 5d of bed rest induced a ~4% loss of skeletal muscle mass in OLD (60-79 y) but not YOUNG (18-28 y) subjects. Identifying muscle transcriptional events in response to bed rest and age-related differences will help identify therapeutic targets to offset muscle loss in vulnerable older adult populations. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

56 Samples

Download data: TXT

(Submitter supplied) The aim of this study was therefore to investigate molecular mechanisms associated with insulin sensitivity in skeletal muscle by relating global skeletal muscle gene expression with a surrogate measure of insulin sensitivity, i.e. homeostatic model assessment of insulin resistance (HOMA-IR). To identify genes with skeletal muscle expression related to insulin sensitivity, we obtained muscle biopsies from 38 non-diabetic participants in study A. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL7144 GPL4133

47 Samples

Download data: CEL, TXT

(Submitter supplied) We studied 9 healthy young non-diabetic men without any family history of diabetes. The mean age and body mass index (BMI) were 25.33 ± 0.33 years and 24.57 ± 0.62 kg/m2, respectively, and the mean 1/ homeostatic model assessment of insulin resistance (HOMA-IR) was 1.17 ± 0.12. We included baseline gene expression profile data (i.e. only before bed rest)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

9 Samples

Download data: TXT

(Submitter supplied) Rationale: Physical inactivity is a risk factor for insulin resistance. We examined the effect of nine days of bed rest on basal and insulin stimulated expression of genes potentially involved in insulin action by applying hypothesis-generating microarray in parallel with candidate gene real-time PCR approaches in 20 healthy, young men. Furthermore, we investigated whether bed rest affected DNA methylation in the promoter region of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) gene. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

60 Samples

Download data: TXT

(Submitter supplied) The present study concerns the effects of 21 days of sustained recumbence (bedrest) and hypoxia, alone and in combination, on skeletal muscle microRNA expression. 14 male subjects participated in 3 experimental campaigns in a counterbalanced fashion: normoxic bedrest (NBR), hypoxic bedrest (HBR) and hypoxic ambulatory confinement (HAMB), both hypoxic conditions with FO2 = 0.141 and PIO2 = 90 ± 0.4 mmHg, equivalent to an altitude of ≈ 4000 m). more...

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

68 Samples

Download data: CEL

(Submitter supplied) Spaceflight results in a number of adaptations to skeletal muscle, including atrophy and shifts towards faster muscle fiber types. To identify changes in gene expression that may underlie these adaptations, microarray expression analysis was performed on gastrocnemius from mice flown on the STS-108 shuttle flight (11 days, 19 hours) versus mice maintained on earth for the same period. Additionally, to identify changes that were due to unloading and reloading, microarray analyses were conducted on calf muscle from ground-based mice subjected to hindlimb suspension (12 days) and mice subjected to hindlimb suspension plus a brief period of reloading (3.5 hours) to simulate the time between landing and sacrifice of the spaceflight mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

23 Samples

Download data: CEL

(Submitter supplied) Following spinal cord injury, skeletal muscle loss is rapid. This severe atrophy is attributed to declines in protein synthesis and increases in protein breakdown. However, the signaling mechanisms controlling these changes are not well understood. Nine male patients and one female patient with spinal cord injury (SCI) (Mean ± SEM = 43.9 ± 6.7 yrs) were recruited for this study. Six patients were quadriplegics and four patients were paraplegics. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

20 Samples

Download data: CEL, CHP

(Submitter supplied) Although short-term disuse does not result in measurable muscle atrophy, studies suggest that molecular changes associated with protein degradation may be initiated within days of the onset of a disuse stimulus. We examined the global gene expression patterns in sedentary men (n = 7, mean age ± S.D = 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3762

Platform:

GPL570

21 Samples

Download data: CEL, CHP

Analysis of left vastus lateralis muscle biopsies from 7 sedentary men following unloading (48 hr) via unilateral lower limb suspensionand and subsequent reloading (24 hr). Results provide insight into molecular events underlying the earliest stages of skeletal muscle disuse and reambulation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 7 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE21496

21 Samples

Download data: CEL, CHP

(Submitter supplied) Older individuals were subjected to complete bed rest for 10 days. Muscle biopsies from the vastus lateralis were obtained immediately prior and at the conclusion of the bed rest. RNA-sequencing was performed to determine the gene expression changes associated with bed rest. Marked decrease in the expression of genes associated with mitochondrial energy metabolism including fatty acid oxidation, TCA cycle and the electron transport chain was observed. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

6 Samples

Download data: XLSX

(Submitter supplied) Massage therapy is commonly used for the physical rehabilitation of skeletal muscle to ameliorate pain and promote recovery from injury. While there is some evidence that massage may relieve pain in injured muscle, the cellular effects remain unknown. To assess the effects of massage, we administered either massage therapy or no treatment to separate quadriceps of eleven young, male participants after exercised-induced muscle damage. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL14871

55 Samples

Download data: PAIR

(Submitter supplied) Microgravity has a dramatic impact on human physiology, illustrated in particular with skeletal muscle impairment. A thorough understanding of the mechanisms leading to loss of muscle mass and structural disorders is necessary for the definition of efficient clinical and spaceflight countermeasures. We investigated the effects of long-term bed rest on transcriptome of soleus (SOL) and vastus lateralis (VL) muscles in healthy women (BRC group, n=8), and the potential beneficial impact of protein supplementation (BRN group, n=8) and of a combined resistance and aerobic training (BRE group, n=8). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6398

170 Samples

Download data: GPR

(Submitter supplied) Chromatin immunoprecipitation sequencing of H3K4me2, H3K27ac as well as, ATACseq and RNA-seq reveals regulatory landscapes across different muscle groups, as well as in response to chronic exercise or muscle PGC1a overexpression. This work defines the unique enhancer repetoire of skeletal muscle in vivo and reveals that highly divergent exercise-induced or PGC1a-driven epigenomic programs direct partially convergent transcriptional networks.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) Chromatin immunoprecipitation sequencing of H3K4me2, H3K27ac as well as, ATACseq and RNA-seq reveals regulatory landscapes across different muscle groups, as well as in response to chronic exercise or muscle PGC1a overexpression. This work defines the unique enhancer repetoire of skeletal muscle in vivo and reveals that highly divergent exercise-induced or PGC1a-driven epigenomic programs direct partially convergent transcriptional networks.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL19057

87 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) Chromatin immunoprecipitation sequencing of H3K4me2, H3K27ac as well as, ATACseq and RNA-seq reveals regulatory landscapes across different muscle groups, as well as in response to chronic exercise or muscle PGC1a overexpression. This work defines the unique enhancer repetoire of skeletal muscle in vivo and reveals that highly divergent exercise-induced or PGC1a-driven epigenomic programs direct partially convergent transcriptional networks.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

15 Samples

Download data: BEDGRAPH, XLSX

(Submitter supplied) Chromatin immunoprecipitation sequencing of H3K4me2, H3K27ac as well as, ATACseq and RNA-seq reveals regulatory landscapes across different muscle groups, as well as in response to chronic exercise or muscle PGC1a overexpression. This work defines the unique enhancer repetoire of skeletal muscle in vivo and reveals that highly divergent exercise-induced or PGC1a-driven epigenomic programs direct partially convergent transcriptional networks.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

62 Samples

Download data: BED, BEDGRAPH, TXT

(Submitter supplied) The β-adrenergic receptor signaling pathway is a major component of adaptive thermogenesis in brown and white adipose tissue during cold acclimation. The β-AR activation highly induces transcriptional coactivator PGC-1α and its splice variant N-terminal (NT)-PGC-1α, promoting the transcription program of mitochondrial biogenesis and thermogenesis. In the present study, we evaluated the role of NT-PGC-1α in brown adipocyte energy metabolism by genome-wide profiling of NT-PGC-1α-responsive genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

4 Samples

Download data: TXT