GEO DataSets

(Submitter supplied) We used microarray to identify the global gene expression profile of recombinant E.coli culture expressing repeating unit of the C. difficile toxin A (rARU) C-terminal region. Controlled condition batch runs were performed with E.coli expressing rARU at both restricted and unrestricted DO conditions for collecting samples of log and late-log phases at unrestricted and restricted DO.

Organism:

Escherichia coli

Type:

Expression profiling by array

Platform:

GPL3154

12 Samples

Download data: CEL

(Submitter supplied) The incidence of Clostridium difficile infection has been steadily rising over the past decade. Its increased rate is associated with the specific NAP1/BI/027 strains which are “hypervirulent” and have led to several large outbreaks since their emergence. However, the relation between their outbreaks and virulence regulation mechanisms remains unclear. It has been reported that the major virulence factor TcdA and TcdB in C. more...

Organism:

Clostridioides difficile R20291

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24374

12 Samples

Download data: XLSX

(Submitter supplied) Overall aim of this study was to investigate the role of up-regulated genes in the generation of cellular stress response that is triggered upon induction of recombinant protein synthesis. Up-regulated genes identified previously by transcriptomic analysis, were knocked out from the host genome and their impact on cellular health and expression capabilities was analyzed. Transcriptomic profiling of the top performing double knock out (∆(elaA+cysW) having significantly higher protein expression levels was compared with the control to demonstrate the ability of this knock-out strain to counter cellular stress.

Organism:

Escherichia coli

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18133

9 Samples

Download data: TXT