GEO DataSets

(Submitter supplied) Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by production of various pathogenic autoantibodies. Increased type I interferon signature is suggested as a trigger of the disease. Previous studies identify increased plasmablasts in peripheral blood of SLE patients. In spite of the unique cellular properties of the plasmablasts compared with other B cell subsets and plasma cells, the biological characteristics of SLE plasmblast remain unknown and few therapeutic strategies targeting SLE plasmablasts have been applicated. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

24 Samples

Download data: TXT

(Submitter supplied) Gene expression profile studies have identified an interferon signature in whole blood or mononuclear cell samples from patients with systemic lupus erythematosus. This study was designed to determine whether specific lymphocyte and myeloid subsets freshly isolated from the blood of systemic lupus erythematosus patients demonstrated unique gene expression profiles compared to subsets isolated from healthy controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4185

Platform:

GPL96

67 Samples

Download data: CEL

Analysis of freshly isolated lymphocyte (CD4+ T cells and CD19+ B cells) and CD33+ myeloid subsets from the blood of Systemic Lupus Erythematosus (SLE) patients. Results provide insight into the molecular mechanisms underlying SLE pathogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell type, 2 disease state sets

Platform:

GPL96

Series:

GSE10325

67 Samples

Download data: CEL

(Submitter supplied) Interferon-alpha Kinoid (IFN-K) is a therapeutic vaccine composed of IFN-alpha2b coupled to a carrier protein. In a phase I/II placebo-controlled trial, we observed that IFN-K significantly decreases the IFN gene signature in whole blood RNA samples from SLE patients (see GSE39088). Here, we analyzed extended follow-up data from IFN-K-treated patients, in terms of persistence of neutralizing anti-IFN± Abs, gene expression profiling and safety. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

52 Samples

Download data: CEL

(Submitter supplied) Patients with systemic lupus erythematosus are characterized by the spontaneous over-expression of interferon(IFN)-induced genes in peripheral blood RNA samples. In the present study, we wanted to study the evolution of the IFN gene signature in the peripheral blood of patients with lupus nephritis, before and after initiation of immunosuppressive therapy. Strikingly, we found that immunosuppressive therapy did not strongly reduce the expression of IFN-induced genes in patients who responded to therapy, as indicated by decreased general and renal indices of disease activity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

30 Samples

Download data: CEL

(Submitter supplied) We performed a phase I/II, randomized, double-blind, placebo-controlled dose-escalation study to examine the safety, immunogenicity, and biological effects of active immunization with interferon alpha-Kinoid (IFN-K) in systemic lupus erythematosus (SLE) patients. Women 18-50 years of age with mild to moderate SLE were immunized with three (n=10) or four doses (n=9) of 30, 60, 120, 240 microgram IFN-K or saline. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

142 Samples

Download data: CEL

(Submitter supplied) Systemic lupus erythematosus (SLE) is a chronic relapsing autoimmune disease characterized by the production of autoantibodies and multiple organ involvement. In this study, we investigated genome-wide DNA methylation changes in the CD8+ T cells from 8 pairs of lupus patients compared to age, sex, and ethnicity matched healthy controls.

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL23976

16 Samples

Download data: IDAT, TXT

(Submitter supplied) SLE patients are always with various disease manifestation. Various cytokines are pointed interacting and playing pathological roles in SLE although the etiopathology is still obscure. In this study, we aimed to investigate the effects of cytokine interactions in the immune response of SLE patients. Overexpressed interferon-inducible(IFI) genes were confirmed in peripheral blood from SLE patients. Using network-based analysis on the immune response-related genes, several networks including cytokines such as TNF and IFN-γ, or beta-estradiol(E2), were constructed. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1291

17 Samples

Download data: TXT

(Submitter supplied) CD52 is a 12 amino acid glycoprotein that is widely expressed on lymphocytes. In T cells, the soluble form of CD52 has been shown to inhibit TCR function by binding Siglec-10. In Monocytes, soluble CD52 has been shown to inhibit TLR signaling and induces apoptosis at high concentrations. To assess the impact of CD52 on B cell function, we left B cells either untreated or exposed to recombinant CD52-Fc for 6 hours prior to lysis and bulk RNA-sequencing. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23227

12 Samples

Download data: TXT

(Submitter supplied) Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by pathogenic auto-antibodies that cause end organ damage. B cells are thought to play a central role in the immunopathogenesis of SLE and display several abnormalities in patients, including a strong type I interferon signature as well as lower expression of surface markers including TLR9 and FcγRIIB. To characterize differences in the proportion of distinct B cell subsets as well as intrinsic transcriptomic differences between B cells from healthy donors and SLE patients, we performed single-cell RNA sequencing on B cells isolated from PBMCs of donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

5 Samples

Download data: TAR

(Submitter supplied) High-throughput sequencing was performed on the PBMCs from SLE patients and healthy control. The purpose of the current study is to identify new genes and pathways of interest involved in the pathogenesis of SLE.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20795

30 Samples

Download data: TXT