GEO DataSets

(Submitter supplied) Schemic retinopathies such as diabetic retinopathy (DR) and retinopathy of prematurity (ROP), are the main causes of blindness in working age and pediatric populations in industrialized countries. It is estimated that close to 100 million individuals worldwide suffer from DR and 15 million preterm infants born each year are predisposed to ROP. Regrettably, relatively little is known of the cellular processes at play during late stages of pathological angiogenesis in these diseases and consequently current standards of care target all neovascularization. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18635

16 Samples

Download data: XLSX

(Submitter supplied) Proliferative retinopathy is associated with abnormal vascular development (neovascularisation) of the retina. In mouse, the oxygen-induced retinopathy (OIR) model mimics the proliferative retinopathy found in extreme premature babies (Retinopathy of Prematurity) and in patients suffering from diabetic retinopathy. Using Drop-seq, we performed single-cell RNAseq analysis of mouse retina under physiological, normoxic (NORM) condition and retina under OIR condition, at postnatal day 14 and day 17, periods associated with a peak of neovascularisation. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

12 Samples

Download data: TXT

(Submitter supplied) Unbiased single-cell RNA-sequencing in freshly-dissociated cells from healthy and stenotic mouse kidneys identified stenotic-kidneys epithelial cells undergoing both mesenchymal transition and senescence.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

8 Samples

Download data: MTX, TSV

(Submitter supplied) Transcriptome of beta-cells isolated from mice expressing p16ink4a and GFP transgenes and of control β-cells isolated from mice expressing only the GFP transgene

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

5 Samples

Download data: TXT

(Submitter supplied) Deregulated retinal angiogenesis directly cause vision loss in many ocular diseases, such as diabetic retinopathy and retinopathy of prematurity. To identify endothelial-specific genes expressed in angiogenic retinal vessels, we purified genetically labeled endothelial cells from Tie2-GFP transgenic mice and performed gene expression profiling using DNA microarray. To find out genes associated with angiogenesis, comparisons of microarray data were carried out between GFP-negative non-endothelial retinal cells and GFP-positive retinal endothelial cells in angiogenic P8 retina.

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4461 GDS4462 GDS4463

Platforms:

GPL82 GPL81 GPL83

18 Samples

Download data: CEL

Analysis of FACS-sorted, GFP-positive endothelial cells from retinas of postnatal day 8 (P8) homozygous Tie2-GFP transgenics. Deregulated retinal angiogenesis causes vision loss in many ocular diseases. Results provide insight into the molecular mechanisms underlying intraretinal angiogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL83

Series:

GSE27238

6 Samples

Download data: CEL

Analysis of FACS-sorted, GFP-positive endothelial cells from retinas of postnatal day 8 (P8) homozygous Tie2-GFP transgenics. Deregulated retinal angiogenesis causes vision loss in many ocular diseases. Results provide insight into the molecular mechanisms underlying intraretinal angiogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL82

Series:

GSE27238

6 Samples

Download data: CEL

Analysis of FACS-sorted, GFP-positive endothelial cells from retinas of postnatal day 8 (P8) homozygous Tie2-GFP transgenics. Deregulated retinal angiogenesis causes vision loss in many ocular diseases. Results provide insight into the molecular mechanisms underlying intraretinal angiogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL81

Series:

GSE27238

6 Samples

Download data: CEL

(Submitter supplied) The healthspan of mice is enhanced by selectively killing senescent cells using a transgenic suicide gene. Achieving the same using small molecules would have a tremendous impact on quality of life and burden of age-related chronic diseases. As senescent cells resist apoptosis, we used microarrays to identify transcripts and pathways that differ between senescent and non-senescent preadipocytes, and might be involved in resistance to apoptosis.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17244

16 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

28 Samples

Download data

(Submitter supplied) In this study we examine the outcomes of p16INK4a activation in the adult epidermis. We found that prolonged expression of p16INK4a induces epidermal hyperplasia and dysplasia. We also found that continued p16INK4a expression increased the number of epidermal papillomas that developed after carcinogen treatment. Profling of p16INK4a- expressing cells showed elevated levels of Wnt-pathway ligands and targets, and pharmacologic or genetic Wnt inhibition suppressed p16-induced hyperplasia. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

7 Samples

Download data: XLSX

(Submitter supplied) In this study we examine the outcomes of p16INK4a activation in the adult epidermis. We found that prolonged expression of p16INK4a induces epidermal hyperplasia and dysplasia. We also found that continued p16INK4a expression increased the number of epidermal papillomas that developed after carcinogen treatment. Profling of p16INK4a- expressing cells showed elevated levels of Wnt-pathway ligands and targets, and pharmacologic or genetic Wnt inhibition suppressed p16-induced hyperplasia. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

21 Samples

Download data: TXT, XLSX

(Submitter supplied) Sustainable muscle regeneration necessitates proper maintenance of the quiescence-reversible SCs pool. Activation of p16Ink4a-associated senescence pathway during aging breaks muscle homeostasis and causes degenerative muscle disease by irreversibly dampening satellite cell (SC) self-renewal capacity. We performed microarrays analysis to compare the genome-wide gene expression profiles of wild-type and Slug-deficient SCs and identified distinct classes of up-regulated genes upon deletion of Slug gene.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

6 Samples

Download data: CEL, CHP

(Submitter supplied) The goal of this study is to identify the senescent cells expressing high level of p16Ink4a (p16Ink4a Hi) in GBMs and characterize their action on the tumor microenvironment at an early timepoint. We introduced the p16-3MR transgene in the GBM mouse model to selectively delete p16Ink4a Hi senescent cells upon ganciclovir (GCV) injection. We compared p16-3MR+GCV to WT+GCV control GBMs that were harvested 7 days after the last GCV injection.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

8 Samples

Download data: SF

(Submitter supplied) The goal of this transcriptomic analysis is to decipher whether NRF2 pathway plays a role in the induction of pro-tumoral senescence in glioblastoma (GBM).

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: SF

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24247 GPL19057

40 Samples

Download data: MTX, SF, TSV, TXT

(Submitter supplied) We performed a scRNAseq analysis conducted at the endpoint of our GBM mouse model. We compared differentially expressed up-regulated genes in p16Ink4a positive vs p16Ink4a negative malignant cells. The goal was to compare this dataset with two other transcriptomic datasets, to explore the upstream regulator(s) of senescence in malignant cells. We found that Nuclear Factor Erythroid 2 Like 2 (Nfe2l2) signaling pathway was enriched in the 3 data sets.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: TXT

(Submitter supplied) The goal of this study is to identify the senescent cells expressing high level of p16Ink4a (p16Ink4a Hi) in GBMs, at an early timepoint. We introduced the p16-3MR transgene in the GBM mouse model to selectively delete p16Ink4a Hi senescent cells upon ganciclovir (GCV) injection. We compared two p16-3MR+GCV to two WT+GCV control GBMs that were harvested 7 days after the last GCV injection. p16Ink4a Hi senescent cells were a subset of malignant cells, mostly grouped in the astrocyte cluster and to a lesser extend in the NP-like cluster. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

4 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) GBM mouse model: The goal of this study is to compare the transcriptomic landscape at the endpoint of GBMs upon senescent cells deletion. We introduced the p16-3MR transgene in the GBM mouse model to selectively delete senescent cells expressing high level of p16Ink4a (p16Ink4a Hi) upon ganciclovir (GCV) injection. We compared p16-3MR+GCV GBMs to two control conditions, either WT+GCV or p16-3MR+GCV. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

18 Samples

Download data: TXT