GEO DataSets

(Submitter supplied) Many transcription factors (TFs) in animals bind to both DNA and mRNA, regulating transcription and mRNA turnover. However, whether plant TFs function at both the transcriptional and posttranscriptional levels remains unknown. The rice (Oryza sativa) bZIP TF AVRPIZ-T-INTERACTING PROTEIN 5 (APIP5) negatively regulates programmed cell death and blast resistance and is targeted by the effector AvrPiz-t of the blast fungus Magnaporthe oryzae. more...

Organism:

Oryza sativa

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24468

3 Samples

Download data: TXT

(Submitter supplied) Rice blast disease is a major threat to rice production worldwide, but the mechanisms underlying rice resistance to the causal agent Magnaporthe oryzae remain elusive. In this whole-genome transcriptome study of rice early defense response to M. oryzae, we applied Affymetrix Rice Genome Genechip to compare the compatible and incompatible rice-M. oryzae interactions in 24 hours post-inoculation.

Organism:

Oryza sativa; Oryza sativa Japonica Group

Type:

Expression profiling by array

Platform:

GPL2025

18 Samples

Download data: CEL

(Submitter supplied) Nucleotide-binding site leucine-rich repeat (NLR) receptors perceive pathogen effectors and trigger plant immunity. However, the mechanisms underlying NLR-triggered defense responses remain obscure. The recently discovered Pigm locus in rice encodes a cluster of NLRs, including PigmR, which confers broad-spectrum resistance to blast fungus. Here, we identify PIBP1 (PigmR-INTERACTING and BLAST RESISTANCE PROTEIN 1), an RRM (RNA-recognition motif) protein that specifically interacts with PigmR and other similar NLRs to trigger blast resistance. more...

Organism:

Oryza sativa

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19290

3 Samples

Download data: TXT

(Submitter supplied) Nucleotide-binding site leucine-rich repeat (NLR) receptors perceive pathogen effectors and trigger plant immunity. However, the mechanisms underlying NLR-triggered defense responses remain obscure. The recently discovered Pigm locus in rice encodes a cluster of NLRs, including PigmR, which confers broad-spectrum resistance to blast fungus. Here, we identify PIBP1 (PigmR-INTERACTING and BLAST RESISTANCE PROTEIN 1), an RRM (RNA-recognition motif) protein that specifically interacts with PigmR and other similar NLRs to trigger blast resistance. more...

Organism:

Oryza sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19290

2 Samples

Download data: TXT

(Submitter supplied) Nucleotide-binding site leucine-rich repeat (NLR) receptors perceive pathogen effectors and trigger plant immunity. However, the mechanisms underlying NLR-triggered defense responses remain obscure. The recently discovered Pigm locus in rice encodes a cluster of NLRs, including PigmR, which confers broad-spectrum resistance to blast fungus. Here, we identify PIBP1 (PigmR-INTERACTING and BLAST RESISTANCE PROTEIN 1), an RRM (RNA-recognition motif) protein that specifically interacts with PigmR and other similar NLRs to trigger blast resistance. more...

Organism:

Oryza sativa

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19290

1 Sample

Download data: TXT

(Submitter supplied) WRKY62 is a transcriptional repressor regulated downstream of WRKY45, a central TF of the salicylic acid (SA) signaling pathway for defense response in rice. To characteraze SA signaling induced genes whose expressions were regulated by WRKY62, we analized genome-wide epression profiles in WRKY62-kd and NB rice using benzothiadiazole (BTH), SA singaling activator.

Organism:

Oryza sativa

Type:

Expression profiling by array

Platform:

GPL8852

8 Samples

Download data: TXT

(Submitter supplied) Benzothiadiazole (BTH) is a so-called ‘plant activator’ and protects plants from diseases by activating the salicylic-acid (SA) signaling pathway. We identified BTH-responsive genes in rice leaves 24 h after treatment using rice 44K microarray. Keywords: response to chemical treatment

Organism:

Oryza sativa

Type:

Expression profiling by array

Dataset:

GDS3070

Platform:

GPL5101

8 Samples

Download data: TXT

Analysis of leaves of Oryza sativa plants treated with benzothiadiazole (BTH). BTH is a functional analog of salicylic acid and enhances the resistance of rice to the fungal pathogen Rhizoctonia solani. Results provide insight into the molecular mechanisms underlying BTH-induced disease resistance.

Organism:

Oryza sativa

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL5101

Series:

GSE7567

8 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are small non-coding RNAs that function as post-transcriptional regulators of gene expression in eukaryotes. In rice, miR7695 targets an alternatively spliced transcript of natural resistance-associated macrophage protein 6 (OsNramp6) encoding an iron transporter whose expression is regulated by infection with the rice blast fungus Magnaporthe oryzae. Rice plants grown under high iron supply showed blast resistance, which supports that iron is a factor in controlling blast resistance by still unknown mechanisms. more...

Organism:

Oryza sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19290

8 Samples

Download data: XLSX

(Submitter supplied) Analysis of transgenic rice overexpressing OsWRKY28, a WRKY type transcription factor. Results provide insight into the role of OsWRKY28 in the defense signaling against rice blast fungus.

Organism:

Oryza sativa

Type:

Expression profiling by array

Platform:

GPL8852

12 Samples

Download data: TXT

(Submitter supplied) Purpose: The aim of this study was to compare transcriptome profiling (RNA-seq) of rice panicle tissue from resistant and suceptible lines at different time intervals infected with M.oryzae. The results were validated using Semi-quantitative PCR. Methods: Panicles from resistant and suceptible rice lines were inoculated with suspension having double distill water, Tween-20 without spores for 0 hour and same suspension with spores was used to inoculate for 48, 72 and 96 hour samples. more...

Organism:

Oryza sativa Indica Group

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14553

16 Samples

Download data: GTF

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by the regulatory factor X protein MoRfx1 by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMorfx1 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

6 Samples

Download data: TXT

(Submitter supplied) chip-seq for MoIug4 in rice, putative transcription factor MoIug4 was secrected from M. oryzae to the nucleus of rice. Here, we performed of MoAtf1 chip-seq assays to uncovered the regulation network

Organism:

Oryza sativa

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9147

1 Sample

Download data: BW

(Submitter supplied) High-throughput sequencing of small RNAs from rice was used to identify distinct miRNAs that are responsive to elicitors from the fungal pathogen Magnaporthe oryzae. [Expression profiling by array] We used microarrays to determine the expression behaviour of target genes for elicitor-regulated miRNAs. [High throughput sequencing] High-throughput sequencing of rice small RNAs was performed in two different tissues, leaves and roots, and two different time point of elicitor treatment, 30' and 2h Amplicons were prepared by 5´and 3´adaptor ligation in which the 5'-adaptor contained a 'barcode' consisting of a 4-nucleotide identifier sequence for each sample. more...

Organism:

Oryza sativa; Oryza sativa Japonica Group

Type:

Expression profiling by array; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL13925 GPL2025

20 Samples

Download data: CEL, FASTA

(Submitter supplied) A T-DNA insertion within RBP-L 3’UTR resulted in 10-25% expression level of RBP-L gene compared to wild-type. The reduced expression of RBP-L caused partial mis-localization of glutelin and prolamine RNAs and conferred other general growth defects including dwarfism, late flowering and smaller seeds. Transcriptome analysis showed that RBP-L knockdown greatly affected the expression of prolamine family genes and many genes invovled in essential biological pathways during plant development.

Organism:

Oryza sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13160

6 Samples

Download data: TXT