GEO DataSets

(Submitter supplied) In many vertebrate species, age at maturity is proportional to organismal lifespan. A number of theories have attempted to provide a conceptual framework for this fascinating apparent co-evolution. The ‘disposable soma’ theory predicts that costly resources that are invested in germline preservation come at the expense of repairing age-related somatic damage. On the other hand, classical experiments in C. more...

Organism:

Nothobranchius furzeri

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30852

23 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Nothobranchius furzeri

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33195 GPL30852

51 Samples

Download data: CSV

(Submitter supplied) Classical evolutionary theories propose tradeoffs between reproduction, damage repair, and lifespan. However, the specific role of the germline in shaping vertebrate aging remains largely unknown. Here, we use the turquoise killifish (N. furzeri) to genetically arrest germline differentiation at discrete stages, and examine how different ‘flavors’ of infertility impact life-history. We first constructed a comprehensive single-cell gonadal atlas, providing cell-type-specific markers for downstream phenotypic analysis. more...

Organism:

Nothobranchius furzeri

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33195

28 Samples

Download data: CSV

(Submitter supplied) This approach was used to increase the continuity of the killifish genome, and assess the relative distance of specific genes from the sex locus.

Organism:

Nothobranchius furzeri

Type:

Other

Platform:

GPL30852

2 Samples

Download data: FASTA, HIC

(Submitter supplied) The plasticity of ageing suggests that longevity may be controlled epigenetically by specific alterations in chromatin state. The link between chromatin and ageing has mostly focused on histone deacetylation by the Sir2 family1, 2, but less is known about the role of other histone modifications in longevity. Histone methylation has a crucial role in development and in maintaining stem cell pluripotency in mammals3. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Datasets:

GDS4575 GDS5012

Platform:

GPL200

23 Samples

Download data: CEL

Analysis of day 8 germline-deficient glp-1 mutants treated with ash-2 RNAi. The ASH-2 trithorax complex trimethylates histone H3 at lysine 4 (H3K4); an intact germline is required for ASH-2-induced lifespan extension. Results provide insight into the role of histone methylation in longevity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL200

Series:

GSE30505

11 Samples

Download data: CEL

Analysis of day 2 germline-deficient glp-1 mutants treated with ash-2 RNAi. The ASH-2 trithorax complex trimethylates histone H3 at lysine 4 (H3K4); an intact germline is required for ASH-2-induced lifespan extension. Results provide insight into the role of histone methylation in longevity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL200

Series:

GSE30505

12 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of C. elegans germline-ablated worms versus unablated intact animals, N2 strain

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL10094

3 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of C. elegans germline-ablated worms versus unablated intact animals, N2 strain

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL10094

3 Samples

Download data: GPR

(Submitter supplied) How lifespan and the rate of aging are set is a key problem in biology. Small RNAs are conserved molecules that impact diverse biological processes through the control of gene expression. However, in contrast to miRNAs, the role of endo-siRNAs in aging remains unexplored. Here, by combining deep sequencing and genomic and genetic approaches in C.CaenorhabditisC. elegans elegans, we reveal an unprecedented role for endo-siRNA molecules in the maintenance of proteostasis and lifespan extension in germline-less animals. more...

Organism:

Caenorhabditis elegans

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18245

6 Samples

Download data: XLSX

(Submitter supplied) How lifespan and the rate of aging are set is a key problem in biology. Small RNAs are conserved molecules that impact diverse biological processes through the control of gene expression. However, in contrast to miRNAs, the role of endo-siRNAs in aging remains unexplored. Here, by combining deep sequencing and genomic and genetic approaches in C. elegans, we reveal an unprecedented role for endo-siRNA molecules in the maintenance of proteostasis and lifespan extension in germline-less animals. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL25800

8 Samples

Download data: TXT

(Submitter supplied) Transcriptomic effects of lin-28 RNAi on N2 and glp-1 worms were compared.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

4 Samples

Download data: DIFF

(Submitter supplied) Investigation of whole genome gene expression level changes in C. elegans rsks-1, daf-2, daf-2 rsks-1 and daf-16; daf-2 rsks-1 mutant compared to the wild-type N2 strain. The mutants have altered longevity phenotypes. The mutants analyzed in this study are further described in Di Chen, Patrick Wai-Lun Li, Benjamin A. Goldstein, Waijiao Cai, Emma Lynn Thomas, Fen Chen, Alan E. Hubbard, Simon Melov and Pankaj Kapahi, Germline Signaling Mediates the Synergistically Prolonged Longevity by Double Mutations in daf-2 and rsks-1 in C. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL17925

47 Samples

Download data: PAIR

(Submitter supplied) To study the effect of dietary restriction on killifish, we generated transcriptomes (RNA-seq) for the livers and brains of male and female killifish that had been subjected to different dietary treatements. 

Organism:

Nothobranchius furzeri

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32658

32 Samples

Download data: CSV

(Submitter supplied) We presents a complete single-cell atlas of aging worms, encompassing both somatic and germ cell types. This atlas was built upon 241K nuclei across the worm lifespan at day 1, 6, 12, and 14 (100%, 99%, 61%, and 14% survival rates, respectively). The worms were aged under normal physiological conditions, without interfering with their reproductive processes.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL26672 GPL27998 GPL32326

28 Samples

Download data: MTX, RDS, TSV

(Submitter supplied) Epigenetic factors guide cell fate decisions and can stabilize development by buffering environmental variation. HP1 proteins have a well-characterized role in heterochromatin packaging and gene regulation, while their function in organismal development is less well understood. Here we used genome-wide expression profiling to assess novel functions of the C. elegans HP1 homologue HPL-2 at specific developmental stages

Organism:

Caenorhabditis elegans

Type:

Expression profiling by genome tiling array

Platform:

GPL5634

12 Samples

Download data: CDF, CEL, TXT

(Submitter supplied) SET-9 and SET-26 are highly homologous paralogs that share redundant function in germline development, but SET-26 alone plays a key role in longevity and heat stress response. SET-26 is broadly expressed, but SET-9 is only detectable in the germline, which likely account for their different biological roles. SET-9 and SET-26 bind to H3K4me3 in vitro and in vivo, and their loss results in broadening of H3K4me3 domains. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19757

32 Samples

Download data: BEDGRAPH, XLSX

(Submitter supplied) SET-9 and SET-26 are highly homologous paralogs that share redundant function in germline development, but SET-26 alone plays a key role in longevity and heat stress response. SET-26 is broadly expressed, but SET-9 is only detectable in the germline, which likely account for their different biological roles. SET-9 and SET-26 bind to H3K4me3 in vitro and in vivo, and their loss results in broadening of H3K4me3 domains. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL18245 GPL19757

24 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) The germ cells of multicellular organisms protect their developmental potential through specialized mechanisms. A shared feature of germ cells from worms to humans is the presence of non-membrane-bound ribonucleoprotein organelles called germ granules. Depletion of germ granules in Caenorhabditis elegans (i.e., P granules) leads to sterility and in some germlines expression of the neuronal transgene unc-119::gfp and the muscle myosin MYO-3. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL13657 GPL18245 GPL22765

29 Samples

Download data: XLSX

(Submitter supplied) Dietary restriction (DR) and loss of the genes rsks-1 and daf-2 increase longevity in C. elegans. Polysome profiling allows actively translated mRNA bound by multiple ribosomes to be isolated and compared to the total mRNA present. In this project, we differentiate transcriptional and translational changes in gene expression in C. elegans by combining polysome profiling and mRNA-sequencing. By comparing gene abundance between the two RNA pools, genes with altered translational regulation under DR and in the mutant can be identified.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

24 Samples

Download data: TXT