GEO DataSets

(Submitter supplied) Copy number gains in genes coding for Rho activating exchange factors as well as losses affecting genes coding for RhoGAP proteins are common in breast cancer, suggesting that elevated Rho signaling may play an important role. Extra copies and overexpression of RhoC also occur, although a role for RhoC overexpression in driving tumor formation has not been assessed in vivo. To this end, we report on the development of a Rosa26 (R26)-targeted Cre-conditional RhoC overexpression mouse (R26-RhoC). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

21 Samples

Download data: TSV, TXT

(Submitter supplied) Mutations in PIK3CA, the gene encoding the p110α subunit of PI3K, induce transformation of mammary epithelial cells (MEC). Studies suggest the transforming action of mutant PIK3CA requires binding to activated receptor tyrosine kinases or adaptors. We examined in transgenic mice if ErbB3, a powerful activator of PI3K, is required for mutant PIK3CA-mediated MEC transformation. ErbB3 loss delayed PIK3CAH1047R-dependent mammary gland hyperplasia, but tumor latency, gene expression and markers of PI3K signaling were unaffected. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11383

12 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL17021 GPL4134

22 Samples

Download data: TXT

(Submitter supplied) validate and study CRISPR/Cas9 knock down line by RNA-seq

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

4 Samples

Download data: TXT

(Submitter supplied) Study gene expression in mouse mammary tumors single cell RNA-seq, study CRISPR/Cas9 knock down line by RNA-seq

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

8 Samples

Download data: TXT

(Submitter supplied) compare expression of two groups of tumors

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

10 Samples

Download data: TXT

(Submitter supplied) Beta-catenin controls both cell adhesion and transcription to facilitate ErbB2-driven mammary tumorigenesis In this study, we used conditional knockout and gene expression approaches to understand global molecular and transciptional changes due to ablation of both functions of beta-catenin.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

15 Samples

Download data: CEL, CHP

(Submitter supplied) To identify genes that may facilitate early steps of ErbB2/Neu-mediated mammary tumorigenesis, we performed comparative microarray analysis of 5- and 10-week bitransgenic mammary glands (LHxMMTV-neu) in triplicate. Keywords: transgenic mouse, erbB2, MMTV-neu, HER2, mammary tumor, breast cancer

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1924

Platform:

GPL1261

6 Samples

Download data

Analysis of mammary glands of 5 and 10-week-old bitransgenics overexpressing oncogenic ErbB2/Neu and luteinizing hormone (LH). Results provide insight into the effect of chronic trophic maintenance of the mammary epithelial cells, induced by LH, on ErbB2/Neu-initiated tumorigenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 age sets

Platform:

GPL1261

Series:

GSE3501

6 Samples

Download data

(Submitter supplied) HER2 (ERBB2) gene amplification and PIK3CA mutations often co-occur in breast cancer, and aberrant activation of the PI3K pathway has been implicated in resistance to HER2-directed therapies. We have created a mouse model of HER2-overexpressing (HER2+), PIK3CAH1047R-mutant breast cancer. Mice expressing both human HER2 and mutant PIK3CA in their mammary glands developed tumors with a significantly shorter latency compared to mice expressing either oncogene alone. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11383

19 Samples

Download data

(Submitter supplied) We wanted to know how introduction of H1047R oncogenic mutation of PIK3CA (p110a subunit of PI3K) in HER2-overexpressing MCF10A human non-transformed mammary epitheial cell lines introduces change in gene expression levels. Our control was HER2-overexpressing MCF10A cell line with wild-type (WT) PIK3CA. Mutated oncogene-induced gene expression signature was measured and compared with that induced by the WT oncogene from cell lines harvested at 70-80% confluency.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15069

4 Samples

Download data: TXT

(Submitter supplied) Although ERBB2 amplification and overexpression is correlated with poor outcome in breast cancer, the molecular mechanisms underlying the aggressive nature of these tumors has not been fully elucidated. To investigate this further, we have used a transgenic mouse model of ErbB2-driven tumor progression (ErbB2KI model) that recapitulates clinically relevant events, including selective amplification of the core erbB2 amplicon. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6585 GPL2872

18 Samples

Download data: TXT

(Submitter supplied) ErbB-2 overexpression and amplification occurs in 15 - 30% of human invasive breast carcinomas associated with poor clinical prognosis. Previously, we have demonstrated that four ErbB-2/Neu tyrosine-autophosphorylation sites within the cytoplasmic tail of the receptor recruit distinct adaptor proteins and are sufficient to mediate transforming signals in vitro. Two of these sites representing the Grb2 (Neu-YB) and Shc (Neu-YD) binding sites can induce mammary tumourigenesis and metastasis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2872

12 Samples

Download data: TXT

(Submitter supplied) The objective of this study was to determine the effect of Thyroid Hormone Responsive Protein Spot14 (Spot14) loss on the gene expression profiles of tumors from MMTV-Polyomavirus middle-T antigen (PyMT) mice. MMTV-PyMT/S14-heterozygous mice were crossed with S14-heterozygous mice and 1 cm tumors from MMTV-PyMT control (wild-type S14) or MMTV-PyMT/S14-null offspring were profiled using Affymetrix gene arrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11533

8 Samples

Download data: CEL

(Submitter supplied) The objective of this study was to determine the effect of Thyroid Hormone Responsive Protein Spot14 (Spot14) overexpression on the gene expression profiles of tumors from MMTV-Neu mice. Hemizygous MMTV-Neu and MMTV-Spot14 mice were bred and 1 cm tumors from Neu control or Neu/Spot14 bitransgenic offspring were profiled using Affymetrix gene arrays. Tumors from Neu/Spot14 mice emerged significantly earlier than controls, but expressed many genes associated with lactogenic differentiation and were not highly metastatic. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11533

22 Samples

Download data: CEL

(Submitter supplied) To identify early events of erbB2-induced mammary tumorigenesis, we compared datasets from 14 genechip experiments including MMTV-neu tumors, preneoplastic neu mammary gland (adjacent neu), and age-matched, wild-type control mammary glands Keywords = transgenic mouse Keywords = MMTV-neu Keywords = erbB2 Keywords = HER2 Keywords = mammary tumor Keywords: ordered

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1222

Platform:

GPL81

14 Samples

Download data

Analysis of cancer progression by profiling of preneoplastic mammary glands and tumors of MMTV-neu animals. Activated neu allele placed under the control of mammary tumor virus promoter to enable mammary specific expression. Results provide insight into the early events of Neu induced tumorigenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 disease state, 2 genotype/variation sets

Platform:

GPL81

Series:

GSE2528

14 Samples

Download data

(Submitter supplied) Overexpression and/or amplification of the ErbB-2 oncogene, as well as inactivation of the tumor suppressor PTEN, are two important genetic events in human breast carcinogenesis. To address the biological impact of conditional inactivation of PTEN on ErbB-2-induced mammary tumorigenesis, we generated a novel transgenic mouse model that utilizes the MMTV promoter to directly couple expression of activated ErbB-2 and Cre recombinase to the same mammary epithelial cell (MMTV-NIC). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2872

18 Samples

Download data: TXT

(Submitter supplied) Gain-of-function mutation of PIK3CA represents one of the most common oncogenic events in human malignancy, making PI3K an attractive target for cancer therapy. Despite the great promise of targeted therapy, drug resistance is likely to develop, causing treatment failure. To elucidate resistance mechanisms to PI3K-targeted therapy, we constructed a mouse model of breast cancer conditionally expressing PIK3CA-H1047R. more...

Organism:

Mus musculus

Type:

Genome variation profiling by SNP array

Platform:

GPL13147

7 Samples

Download data: CEL

(Submitter supplied) The transcription factor STAT3 is constitutively activated in tumors of different origin but the molecular bases for STAT3 addiction of tumor cells have not yet been clearly identified. We generated knock/in mice carrying the constitutively active Stat3 allele, Stat3C, and showed that Stat3C could enhance Neu oncogenic power, triggering the production of earlier onset, more invasive mammary tumors. Tumor-derived cell lines displayed higher migration and invasion and disrupted distribution of cell-cell junction markers. The tensin family member Cten (C-Terminal Tensin-like), known to mediate EGF-induced migration and highly expressed in inflammatory breast cancer, was up-regulated in both Neu;Stat3C cells and tumors. Both Cten expression and enhanced migration were strictly dependent on Stat3, and Cten silencing normalized cell migration and rescued cell-cell contact defects. Importantly, the pro-inflammatory cytokine IL-6 could mediate Cten induction in MCF10 cells, in an exquisitely Stat3-dependent way. This model allowed us to shed some light on the oncogenic role of Stat3 in the breast, suggesting moreover a mechanism through which inflammatory signals can cooperate with EGF receptors in inflammatory breast cancer.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6105

12 Samples

Download data: TXT