GEO DataSets

Analysis of the polysomal maternal mRNA (MmRNA) populations of oocytes and late one-cell stage embryos. Results provide insight into the mechanisms that regulate the stage-specific translation of MmRNAs in early development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 development stage sets

Platform:

GPL1261

Series:

GSE3962

8 Samples

Download data

(Submitter supplied) Transcriptional activation in mammalian embryos occurs in a stepwise manner. In mice, it begins at the late one-cell stage, followed by a minor wave of activation at the early two-cell stage, and then the major genome activation (MGA) at the late two-cell stage. Cellular homeostasis, metabolism, cell cycle, and developmental events are orchestrated before MGA by time-dependent changes in the array of maternal transcripts being translated (i.e., the translatome). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2387

Platform:

GPL1261

8 Samples

Download data

(Submitter supplied) Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remains unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. more...

Organism:

Mus musculus

Type:

Other

Platform:

GPL24247

15 Samples

Download data: BW, CSV, TSV

(Submitter supplied) Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remains unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. more...

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL21103

192 Samples

Download data: TSV

(Submitter supplied) Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remain unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing; Other

Platforms:

GPL21103 GPL24247

602 Samples

Download data: BW, TSV

(Submitter supplied) Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remains unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

24 Samples

Download data: CSV, TSV

(Submitter supplied) Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remains unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

371 Samples

Download data: CSV, TSV

(Submitter supplied) The earliest stage of animal development is controlled by maternally deposited mRNA transcripts and proteins. Once the zygote is able to transcribe its own genome, maternal transcripts are degraded, in a tightly regulated process known as the maternal to zygotic transition (MZT). While this process has been well-studied within model species, we have little knowledge of how the pools of maternal and zygotic transcripts evolve. more...

Organism:

Drosophila mauritiana; Drosophila mojavensis; Drosophila pseudoobscura; Drosophila willistoni; Drosophila persimilis; Drosophila santomea; Drosophila ananassae; Drosophila erecta; Drosophila miranda; Drosophila sechellia; Drosophila simulans; Drosophila virilis; Drosophila melanogaster; Drosophila yakuba

Type:

Expression profiling by high throughput sequencing; Third-party reanalysis

14 related Platforms

119 Samples

Download data: TXT

(Submitter supplied) Translational regulation plays an important role in gene expression and function. Although the transcriptional dynamics of mouse pre-implantation embryos has been well characterized, the global mRNA translation landscape and the master regulators of zygotic genome activation (ZGA) remain unknown. Here, by developing and applying a low-input ribosome profiling (LiRibo-seq) technique, we profiled the mRNA translation landscape in mouse pre-implantation embryos and revealed the translational dynamics during mouse pre-implantation development. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:

GPL19057 GPL13112

50 Samples

Download data: BIGWIG, TXT

(Submitter supplied) mRNA seq based approach to determine the transcriptome dynamics during early development To study the mechanisms regulating this developmental event in zebrafish, we applied RNA deep sequencing technology and generated comprehensive transcriptome profiles of 6 developmental stages from oocyte to early gastrulation. We determined the expression levels of maternal and zygotic transcripts and clustered them based on expression pattern. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10658

6 Samples

Download data: BEDGRAPH, GFF

(Submitter supplied) The Ca2+ oscillations initiated by the fertilizing sperm (but terminating concomitant with pronucleus formation) apparently ensure that the events constituting egg activation occur in the correct temporal order; early events (e.g., cortical granule exocytosis) require fewer oscillations than later events (e.g., recruitment of maternal mRNA). Whether the Ca2+ signaling events impact long-term development, in particular development to term, is unknown. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3293

Platform:

GPL1261

12 Samples

Download data: CEL

Analysis of blastocysts receiving reduced (CFW) or excess (20-p) Ca2+ signalling events. Results provide insight into the molecular mechanisms underlying the differential effect of the CFW and 20-p treatments on the ability of blastocysts to implant or develop post-implantation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 protocol sets

Platform:

GPL1261

Series:

GSE11687

12 Samples

Download data: CEL

(Submitter supplied) Understanding preimplantation development is important both for basic reproductive biology and for practical applications including regenerative medicine and livestock breeding. Global expression profiles revealed and characterized the distinctive patterns of maternal RNA degradation and zygotic gene activation, including two major transient waves of de novo transcription. The first wave corresponds to zygotic genome activation (ZGA); the second wave, named mid-preimplantation gene activation (MGA), precedes the dynamic morphological and functional changes from the morula to blastocyst stage. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS578 GDS579

Platform:

GPL870

64 Samples

Download data

Characterization of zygotic gene activation (ZGA) and influence of maternal transcripts. Embryos cultured until 2-cell stage with inhibitors of transcription (α-amanitine), DNA replication (aphidicolin) and protein synthesis (cycloheximide).

Organism:

Mus musculus

Type:

Expression profiling by array, log ratio, 5 agent, 4 development stage sets

Platform:

GPL870

Series:

GSE936

36 Samples

Download data

Dynamics of global gene expression changes during preimplantation development. Unfertilized eggs, fertilized egg, 2-cell embryos, 4-cell embryos, 8-cell embryos, morulae and blastocysts examined.

Organism:

Mus musculus

Type:

Expression profiling by array, log ratio, 7 development stage sets

Platform:

GPL870

Series:

GSE936

28 Samples

Download data

(Submitter supplied) An embryo starts its life with maternal mRNA clearance, which is crucial for embryonic development. The elimination of maternal transcripts occurs by the joint action of two pathways: the first is a maternally encoded mRNA decay pathway (M-decay), while the second is zygotic genome activation (ZGA)-dependent pathway (Z-decay). However, the zygotic factors triggering maternal mRNA decay in early mammalian embryos remain largely unknown. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21273

15 Samples

Download data: TXT

(Submitter supplied) We applied BS-seq to mouse GV and MII oocytes, and 1-Cell embryos.

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL21103

3 Samples

Download data: CSV

(Submitter supplied) (1) We cloned and transfected human xenogenic (zebrafish, mouse, etc.) NSUN2 into human NSUN2 knockout cells.(2) We treated wild-type human cells with nocodazole.

Organism:

Homo sapiens

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL20301

5 Samples

Download data: CSV

(Submitter supplied) We applied RNA-seq to examine the effect of NSUN2 knockout on gene expression changes

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21306

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Drosophila melanogaster; Xenopus tropicalis; Homo sapiens; Danio rerio; Xenopus laevis; Mus musculus

Type:

Methylation profiling by high throughput sequencing

6 related Platforms

67 Samples

Download data: CSV