GEO DataSets

Analysis of cultured airway epithelial cells (AEC) from matrix metalloproteinase (MMP) -7 or -10 mutants after P. aeruginosa infection. MMP-7 and MMP-10 are induced by acute P. aeruginosa pulmonary infection. Results provide insight into the roles of the MMPs in the host response to Pseudomonas.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 genotype/variation, 2 infection, 3 time sets

Platform:

GPL1261

Series:

GSE7957

25 Samples

Download data: CEL

(Submitter supplied) Airway epithelium is the initial point of host-pathogen interaction in Pseudomonas aeruginosa infection, an important pathogen in cystic fibrosis and nosocomial pneumonia. We used global gene expression analysis to determine airway epithelial transcriptional responses dependent on matrilysin (MMP-7) and stromelysin-2 (MMP-10), two matrix metalloproteinases induced by acute P. aeruginosa pulmonary infection. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3040

Platform:

GPL1261

25 Samples

Download data: CEL

(Submitter supplied) Several members of the matrix metalloproteinase (MMP) family control specific immune processes, such as leukocyte influx and chemokine activity. Stromelysin-2 (MMP10) is expressed in numerous tissues after injury; however, little is known of its function. Here, we report that MMP10 is expressed by macrophages in human lungs from patients with cystic fibrosis and induced in mouse macrophages in response to Pseudomonas aeruginosa infection both in vivo and in cultured bone marrow-derived macrophages (BMDM). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

18 Samples

Download data: CEL

(Submitter supplied) Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR Findings: 20-30 million reads were mapped per sample and transcripts were identifed that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

48 Samples

Download data: CSV

(Submitter supplied) This series contains 5 groups of U133A arrays with targets from Calu-3 cell line post-infection with P aeruginosa strains. Calu-3 human lung epithelial cells were seeded onto 6-well plates and grown to about 80% confluency. Epithelial monolayers were infected with 108 CFUs per ml of each bacterial strain for 60 min and then washed extensively with phosphate-buffered saline. The cultures were subsequently incubated in fresh RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 µg/ml gentamicin. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS858

Platform:

GPL96

19 Samples

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Comparison of lung epithelial Calu-3 cells infected with the mucoid alginate-producing FRD1 strain to those infected with the isogenic motile flagellin-producing FRD440 strain of Pseudomonas aeruginosa. Mucoid and motile strains are associated with chronic and acute lung infections, respectively.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 genotype/variation, 5 infection sets

Platform:

GPL96

Series:

GSE923

19 Samples

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(Submitter supplied) Global expression profiling of airway epithelial cells infected with Pseudomonas aeruginosa and the rsmA mutant. Recent work in our laboratory investigated the impact of RsmA on a number of airway epithelial cell phenotypes including actin depolymerization, cytotoxicity and invasion. These cellular phenotypes were influenced by the positive effect of RsmA on the expression of the TTSS in P. aeruginosa (Mulcahy et al. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2287

Platform:

GPL96

6 Samples

Download data: CEL, EXP

Analysis of airway epithelial cells infected with Pseudomonas aeruginosa PAO1 or the rsmA mutant. RsmA is a small RNA-binding protein that plays a role in the regulation of virulence-related genes in P. aeruginosa. Results provide insight into the influence of RsmA-regulated functions on the host.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 genotype/variation, 2 infection sets

Platform:

GPL96

Series:

GSE4485

6 Samples

Download data: CEL, EXP

(Submitter supplied) Matrix metalloproteinase 7 (MMP7) is expressed at low levels in intact, normal airways by non-mucous-producing cells, including ciliated cells. In response to injury and infection, MMP7 expression is quickly and markedly upregulated and functions to regulate wound repair and various mucosal immune processes. We evaluated the global transcriptional response of airway epithelial cells from wild type and Mmp7-null mice cultured at an air-liquid interface. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

16 Samples

Download data: CEL, CHP

(Submitter supplied) Pseudomonas aeruginosa is an opportunistic pathogen that causes nosocomial pneumonia and infects patients with cystic fibrosis. P. aeruginosa lung infections are difficult to treat due to bacterial resistance to antibiotics, and strains with multi-drug resistance are becoming more prevalent. Here we examined the use of a small host defense peptide, innate defense regulator 1002 (IDR-1002), in an acute P. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL11002 GPL17021

32 Samples

Download data: TXT

(Submitter supplied) Comparison of libraries derived from primary human bronchial epithelial cells which are [i] unstimulated, [ii] stimulated with the pro-inflammatory cytokines IL1beta and TNFalpha and [iii] stimulated with heat-inactivated Pseudomonas aeruginosa (strain PAO1). Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

3 Samples

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(Submitter supplied) An NF-κB target gene array (Panomics, Inc., Redwood City, CA) was performed to profile the PAO1-dependent expression pattern of 111 NF-kB-regulated genes in primary human airway epithelial cells (HAECs). A small scale microarray was carried out using NF-κB target gene array kit (Panomics, Inc., Redwood City, CA). A long sense-strand oligonucleotide for each of the 111 human genes that have been previously shown to be regulated by NF-KB signaling pathway was spotted in duplicate on the nitrocellulose membrane. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5807

2 Samples

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(Submitter supplied) To gain insights into the initial phases of P. aeruginosa infections and to identify P. aeruginosa genes regulated in response to respiratory epithelia we exposed P. aeruginosa to cultured primary differentiated human airway epithelia. We used a P. aeruginosa strain (PAO1) that causes acute damage to the epithelia and a mutant (PAOSC11) with defects in Type III secretion and in rhamnolipid synthesis. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2502

Platform:

GPL84

8 Samples

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Analysis of Pseudomonas aeruginosa PAO1 or Type III secretion system (TTSS), rhamnolipid synthesis double mutant strain exposed to cultured airway epithelia. TTSS is a virulence factor in acute infections. Strains from chronic infections express decreased levels of TTSS or lack a functional TTSS.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, count, 2 agent, 2 strain sets

Platform:

GPL84

Series:

GSE4614

8 Samples

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(Submitter supplied) Wild-type and dF508 CFTR mutant human bronchial epithelial cells were uninfected or infected for 3 hours at an MOI of 30-50 with P.aeruginosa strain PAO1. Each cell line was tested in 4 replicates. Each replicate consists of 4 data sets: 2 sets of duplicate spots on 2 arrays. The two arrays in each replicate were dye-swapped pairs where the opposite dye was used for each sample in the second array. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1883

32 Samples

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