GEO DataSets

Analysis of LCM-harvested high-grade squamous intraepithelial lesions (HSIL) and invasive squamous cell carcinomas (SCC) from cervices. If left untreated, a subset of the HSILs will progress to SCCs. Results provide insight into the molecular mechanisms underlying cervical cancer progression.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 disease state sets

Platform:

GPL96

Series:

GSE7803

38 Samples

Download data: CEL

(Submitter supplied) 10 normal squamous cervical epitheilia samples, 7 high grade squamous intraepithelial lesions, and 21 invasive squamous cell carcinomas of the cervix samples were obtained using laser capture miicrodissection. Two rounds of T7-based linear RNA amplification using the Arcturus RiboAmp kit were performed for each sample, and assayed using Affymetrix HG_U133A arrays. Keywords: disease state analysis

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3292

Platform:

GPL96

41 Samples

Download data: CEL, TXT, XLS

(Submitter supplied) We sought to apply the technologies of gene expression profiling to detect genes significant in the aetiology of cervical carcinoma . We investigated 14 normal (NAD), 11 low grade squamous intrapepithelial lesions (LSIL), 21 high grade squamous intraepithelial lesions (HSIL) and 28 squamous cell carcinomas by Affymetrix GeneChip whole transcriptome profiling. Two SCC cell lines were also included in the cohort. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL571

77 Samples

Download data: CEL

(Submitter supplied) The type II Oncostatin M receptor (OSMR) serves as the main binding site for the pleiotropic cytokine OSM. We have previously demonstrated a positive correlation between copy number driven OSMR over-expression and adverse clinical outcome in cervical tumours and have also established enhanced angiogenic, migratory and invasive potential as major consequences of OSMR over-expression using cell-line models of cervical cancer. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

72 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array; Genome variation profiling by array; Expression profiling by array

5 related Platforms

200 Samples

Download data: CEL, GPR

(Submitter supplied) A better understanding of the consequences of recurrent (epi)genetic alterations that occur during cervical carcinogenesis is essential in the search for novel biomarkers. In this study we determined genome-wide expression profiles of 10 squamous cell carcinomas (SCCs), 5 adenocarcinomas (AdCAs) and 6 normal epithelial samples. Expression patterns were subsequently combined with previously determined chromosomal profiles in the same carcinomas. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array; Expression profiling by genome tiling array

Platforms:

GPL3054 GPL3441

38 Samples

Download data

(Submitter supplied) Detection of copy number dependent gene expression profiles in epithelial tumours has lead to the discovery of genes significant in diagnosis, prognosis and of therapeutic value. We sought to apply the technologies of gene expression profiling and copy number aberration detection by high density oligonucleotide microarray analysis to detect genes with expression profiles regulated by copy number. We surveyed copy number aberrations in 27 squamous cell carcinomas tissues and ten cervical carcinoma cell lines by Agilent 244k aCGH, and in a companion study of 77 tissues we obtained transcriptional profiles of a broader ranger of histologies. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by array

Platform:

GPL9128

37 Samples

Download data: GPR, TXT

(Submitter supplied) For many oncogenes, increased expression resulting from copy number gain confers a selective advantage to cells that consequently make up the tumour bulk. To identify oncogenes of potential biological significance in cervical squamous cell carcinoma (SCC), 36 primary samples and ten cell lines were screened by array comparative genomic hybridization (CGH). The most commonly occurring regions of copy number gain that also showed amplification were 5p15.2–14.3 (59%), 5p13.3 (65%), and 5p13.2–13.1 (63%). more...

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platform:

GPL13177

40 Samples

Download data: GPR

(Submitter supplied) For many oncogenes, increased expression resulting from copy number gain confers a selective advantage to cells that consequently make up the tumour bulk. To identify oncogenes of potential biological significance in cervical squamous cell carcinoma (SCC), 36 primary samples and ten cell lines were screened by array comparative genomic hybridization (CGH). The most commonly occurring regions of copy number gain that also showed amplification were 5p15.2–14.3 (59%), 5p13.3 (65%), and 5p13.2–13.1 (63%). more...

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platform:

GPL13176

46 Samples

Download data: GPR, TXT

(Submitter supplied) Although gain of chromosome-5p is one of the most frequent DNA copy number imbalances in cervical squamous cell carcinoma (SCC), the genes that drive its selection remain poorly understood. In a previous cross-sectional clinical study we showed that the microRNA processor Drosha (located on chromosome-5p) demonstrates frequent copy-number gain and over-expression in cervical SCC, associated with altered microRNA profiles. more...

Organism:

JC polyomavirus; Human immunodeficiency virus 1; Merkel cell polyomavirus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Lymphocryptovirus; Homo sapiens; Human betaherpesvirus 5; Rhadinovirus; Betapolyomavirus hominis; Betapolyomavirus macacae

Type:

Non-coding RNA profiling by array; Expression profiling by array

Platforms:

GPL11338 GPL570

24 Samples

Download data: CEL, TXT

(Submitter supplied) Comparison of mRNA expression profiles in W12 Series 1 cervical ectokeratinocytes at passage number 22 versus 19 (during which time the cells gain an invasive phenotype) As these cells demonstrate gain of chromosome 5p during this time, mRNA expression profiling data interrogated for over-expressed genes on 5p that may be important in cervical neoplastic progression.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) Comparison of miRNA expression profiles in cervical carcinoma cell lines to study the effects of Drosha expression levels

Organism:

Human alphaherpesvirus 2; Lymphocryptovirus; Merkel cell polyomavirus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Human immunodeficiency virus 1; Homo sapiens; Rhadinovirus; Betapolyomavirus hominis; JC polyomavirus; Betapolyomavirus macacae

Type:

Non-coding RNA profiling by array

Platform:

GPL11338

18 Samples

Download data: TXT

(Submitter supplied) A microarray-based strategy using digestion with the methylation-sensitive restriction enzyme HpaII, ligation, and PCR, was performed to assess the DNA methylation status of promoter regions in Six human cervical adenocarcinomas (ACA) and four squamous cell carcinomas (SCC). Two array platforms were used; an array of 11,994 ~1.5kb PCR products from 10,445 promoter regions, and an array of 355,264 oligonucleotides for 18,212 HpaII fragments in 12,617 promoter regions. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by array

Platforms:

GPL6442 GPL8286 GPL6441

24 Samples

Download data: TXT

(Submitter supplied) Cells located at the invasive front and in the tumor mass of mouse cervical squamous cell carcinomas were isolated by laser microdissection from hematoxylin-stained HPV/E2 sections. 7 months old HPV/E2 mice treated with oestrogen develop invasive cervical squamous cell carcinomas. This model recapitulates human invasive cervical neoplasias. We used microarrays to compare the transcriptome of cells located at the invasive front versus cells located in the tumor mass of mouse squamous cell carcinomas.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) The development of an HPV-associated cervical cancer usually takes a long time (years, decades) and comprises stepwise change of intraepithelial (preclinical) neoplastic forms, but after invasive carcinoma is established it demonstrates extremely aggressive behavior and heterogenic molecular profile. Women with pre-invasive neoplastic lesions or early-stage invasive cervical cancer who underwent treatment in the Republican Oncological Dispensary were enrolled in our study (all were HPV-positive). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

16 Samples

Download data: TXT

(Submitter supplied) Background. MicroRNAs (miRNAs) are short (~22 nt) non-coding regulatory RNAs that control gene expression at the translational level. Deregulation of miRNA expression has been discovered in a wide variety of tumours and it is now clear that they contribute to cancer development and progression. This prompted the development of miRNA-chips for cancer diagnosis or prognosis, opening a new door to understand carcinogenesis. more...

Organism:

Mus musculus; Rattus norvegicus; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL7534

43 Samples

Download data: TXT