GEO DataSets

Analysis of small intestines of male C57BL/6J rodents fed a powdered high-fat purified diet for up to 8 weeks. Diet contributes to obesity and insulin resistance (IR) development. Results provide insight into the molecular mechanisms underlying dietary fat-induced obesity and IR pathogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 3 time, 3 tissue sets

Platform:

GPL1261

Series:

GSE8582

18 Samples

Download data: CEL, EXP

(Submitter supplied) Obesity and insulin resistance are two major risk factors underlying the metabolic syndrome. To gain more insight in the role of the small intestine in the etiology of these metabolic disorders, a microarray study was performed on small intestines (SI) of C57BL/6J mice that were fed a high fat diet mimicking the fatty acid composition of a Western-style human diet. The mice became obese and developed dietary fat-induced glucose intolerance. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3357

Platform:

GPL1261

18 Samples

Download data: CEL, EXP

(Submitter supplied) ABSTRACT Background & aims: The role of the intestine in the maintenance of cholesterol homeostasis is increasingly recognized. Fecal excretion of cholesterol is the last step in the atheroprotective reverse cholesterol transport (RCT) pathway, to which both biliary and transintestinal cholesterol excretion (TICE) contribute. The mechanisms controlling the flux of cholesterol through the TICE pathway are,however, poorly understood. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

24 Samples

Download data: TXT

(Submitter supplied) Small Heterodimer Partner (SHP/NR0B2) is an unusual orphan nuclear receptor that does not have a DNA binding domain and acts as a co-repressor for many transcriptional factors, including LRH-1, SREBPs, FOXA1, and AhR, which inhibits expression of its target genes. To explore global intestinal functions of SHP, WT and SHP-KO mice were fed for 6 h after fasting overnight. In SHP-KO mice, 1,707 genes were upregulated, and 1,055 genes downregulated by 2-fold or more compared to WT mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: TXT

(Submitter supplied) HSL is a key enzyme in in the mobilization of fatty acids from the triglyceride stores of white adipose tissue. In addition, it is expressed in mice liver. In the present microarray study, changes in the transcript profile of murine liver samples due to global HSL knockout were investigated. Keywords: genetic modification

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3508

Platform:

GPL81

4 Samples

Download data: CEL, EXP

Analysis of livers from hormone-sensitive lipase (HSL) null mutants fed a high fat diet. Decreased hepatic insulin sensitivity and elevation of plasma cholesterol are observed in this HSL-null animal model. Results provide insight into the mechanisms underlying these features.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 protocol sets

Platform:

GPL81

Series:

GSE10067

4 Samples

Download data: CEL, EXP

(Submitter supplied) Except for conversion to bile salts, there is no major cholesterol degradation pathway in mammals. Efficient excretion from the body is therefore a crucial element in cholesterol homeostasis. Yet, the existence and importance of these pathways in humans is a matter of debate. We quantified cholesterol fluxes in 15 male volunteers using a cholesterol balance approach. Ten participants repeated the protocol after 4-weeks treatment with ezetimibe, an inhibitor of intestinal and biliary cholesterol absorption. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

13 Samples

Download data: TXT

(Submitter supplied) Background: The selective absorption of nutrients and other food constituents in the small intestine is mediated by a group of transport proteins and metabolic enzymes, often collectively called ‘intestinal barrier proteins’. An important receptor that mediates the effects of dietary lipids on gene expression is the peroxisome proliferator-activated receptor alpha (PPARα), which is abundantly expressed in enterocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

35 Samples

Download data: CEL

(Submitter supplied) Gene expression regulation of transporters and phase I/II metabolic enzymes in murine small intestine during fasting Keywords: metabolic state analysis

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2934

Platform:

GPL339

12 Samples

Download data: CEL

Analysis of PPARalpha deficient small intestines after a 24 hour fast. PPARalpha is a fatty acid-activated transcription factor. Results provide insight into transport function and phase I/II metabolism in the small intestine during fasting and the role of PPARalpha in the response to fasting.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 protocol sets

Platform:

GPL339

Series:

GSE6864

12 Samples

Download data: CEL

(Submitter supplied) There is increased interest in the potential protective role of dietary Ca in the development of metabolic disorders related to the metabolic syndrome. Ca-induced intestinal precipitation of fatty acids and bile acids as well as systemic metabolic effects of Ca on adipose tissue is proposed to play a causal role. In this experiment, we have studied all these aspects to validate the suggested protective effect of Ca supplementation, independent of other dietary changes, on the development of diet-induced obesity and insulin resistance. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3831

Platform:

GPL1261

6 Samples

Download data: CEL, EXP

Analysis of small intestine (proximal, middle, distal) mucosal cells of C57BL/6J males fed high-fat diets differing in Ca concentration. High-Ca (150mmol/kg) stimulates fecal fat and bile acid excretion. Results provide insight into role of dietary Ca on development of obesity or insulin resistance.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol, 3 tissue sets

Platform:

GPL1261

Series:

GSE18581

6 Samples

Download data: CEL, EXP

(Submitter supplied) 8 week-old male Hsd:ICR(CD-1) mice were fed ad libitum a standard chow (Harlan Teklad diet 2018S) and housed in groups of five. Animals were then divided into 3 pools (n=10) and sacrificed. The small intestine was extracted and divided into three sections, where the first 2-3 cm after the stomach comprised the duodenum, the middle third the jejunum, and the section before the ileo-ceco-colic junction comprised the ileum. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS521 GDS522 GDS524

Platforms:

GPL82 GPL83 GPL81

36 Samples

Download data

Analysis of regional differences in gene expression profiles along the intestinal tract. Duodenum, jejunum, ileum, and colon of 8 week-old male Hsd:ICR(CD-1) mice examined.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 tissue sets

Platform:

GPL83

Series:

GSE849

12 Samples

Download data

Analysis of regional differences in gene expression profiles along the intestinal tract. Duodenum, jejunum, ileum, and colon of 8 week-old male Hsd:ICR(CD-1) mice examined.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 tissue sets

Platform:

GPL82

Series:

GSE849

12 Samples

Download data

Analysis of regional differences in gene expression profiles along the intestinal tract. Duodenum, jejunum, ileum, and colon of 8 week-old male Hsd:ICR(CD-1) mice examined.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 tissue sets

Platform:

GPL81

Series:

GSE849

12 Samples

Download data

(Submitter supplied) We studied the effect of dietary fat type, varying in polyunsaturated/saturated fatty acid ratio's (P/S) on development of metabolic syndrome. C57Bl/6J mice were fed purified high-fat diets (45E% fat) containing palm oil (HF-PO; P/S 0.4), olive oil (HF-OO; P/S 1.1) or safflower oil (HF-SO; P/S 7.8) for 8 weeks. A low-fat palm oil diet (LF-PO; 10E% fat) was used as a reference. Additionally, we analyzed diet-induced changes in gut microbiota composition and mucosal gene expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4339

Platform:

GPL1261

9 Samples

Download data: CEL

Analysis of small intestine divided into 3 equal parts along the proximal to distal axis (SI 1, SI 2, SI 3) from C57Bl/6J males fed high-fat diets varying in polyunsaturated/saturated fatty acid ratios (P/S). Results provide insight into role of dietary fat type on development of metabolic syndrome.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 agent, 3 tissue sets

Platform:

GPL1261

Series:

GSE18586

9 Samples

Download data: CEL

(Submitter supplied) Excessive intake of dietary fat is known to be a contributing factor in the development of obesity. In this study, we determined the dose-dependent effects of dietary fat on the development of this metabolic condition with a focus on changes in gene expression in the small intestine. C57BL/6J mice were fed diets with either 10, 20, 30 or 45 energy% (E%) derived from fat for four weeks (n=10 mice/diet). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7440

120 Samples

Download data: CEL

(Submitter supplied) Background Small intestine and liver greatly contribute to whole body lipid, cholesterol and phospholipid metabolism but to which extent cholesterol and phospholipid handling in these tissues is affected by high fat Western-style obesogenic diets remains to be defined. We therefore quantified cholesterol and phospholipid concentrations in intestine and liver and determined fecal neutral sterol and bile acid excretion in C57Bl/6N mice fed for 12 weeks either a cholesterol-free high carbohydrate control diet or a high fat diet containing 0.03 % (w/w) cholesterol. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7441

24 Samples

Download data: CEL