GEO DataSets

Analysis of mechanisms of clofibric acid (CLO) induced hepatocarcinogenesis. Whole liver and early pre-neoplastic foci isolated by laser capture microdissection (LCM) examined.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 5 cell type, 3 dose sets

Platform:

GPL85

Series:

GSE691

52 Samples

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(Submitter supplied) This is an evaluation of the impact of the successive steps of LCM procedure on gene expression profiling by comparing profiles from LCM samples to those obtained with non-microdissected liver samples collected after a one month CLO treatment in the rat. We showed that the H&E staining used for identifying PP-foci and the laser microdissection itself do not impact on RNA quality. Keywords = Microdissection Keywords = Clofibric Acid Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS518

Platform:

GPL85

52 Samples

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(Submitter supplied) F344 rats were divided into 4 groups: vehicle control, DEN, DEN+CLO and CLO. After one week of basal diet, rats belonging to the groups DEN and DEN+CLO underwent intraperitoneal injection of DEN (30mg/kg body weight) dissolved in NaCl 9‰. The two other groups were injected with NaCl 9‰ alone. At 30mg/kg, DEN is non-necrogenic thus only exhibiting initiating properties. Twelve days after injection, diet from rats belonging to the groups CLO and DEN+CLO was changed for diet containing 5000ppm CLO for up to 608 days. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL341

176 Samples

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(Submitter supplied) Samples and RNA preparation: The series is composed of 4 hybridizations for analysis of differentially expressed genes in the liver of ciprofibrate dosed vs. control rats. Rats (200-250 g) were given (by gastric intubation) tap water (control group, n=5) or ciprofibrate (50 mg/kg body weight, once daily for 60 days, n=4). Liver total RNA extraction was performed first by ultracentrifugation on a cesium chloride cushion and then using TRIZOL Reagent (GIBCO BRL Life Technologies, New York, NY). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL264

4 Samples

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(Submitter supplied) Liver gene expression was examined in male cynomolgus monkeys treated with ciprofibrate (PPAR-alpha agonist) for 4 days at 400 mg/kg/day and treated for 15 days at 0, 3, 30, 150 or 400 mg/kg/day. The untreated control group were given only the vehicle (0.5% hydroxypropyl methylcellulose). Two animals per group were used for the 4 day treatment and four animals per group were used for the 15 day treatment (except the 15 day control group, which had three animals). more...

Organism:

Homo sapiens; Macaca fascicularis

Type:

Expression profiling by array

Dataset:

GDS1442

Platform:

GPL8300

23 Samples

Download data: CEL

Analysis of primate livers exposed to ciprofibrate at various doses for 4 or 15 days. Peroxisome proliferator-activated receptor-α (PPARα) agonists such as ciprofibrate cause hepatocarcinogenesis in rodents but not in primates. Results identify species differences in response to PPARα agonist.

Organism:

Macaca fascicularis; Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 5 dose, 2 time sets

Platform:

GPL8300

Series:

GSE2853

23 Samples

Download data: CEL