GEO DataSets

(Submitter supplied) Exploiting the symbiotic interaction between crops and nitrogen-fixing bacteria is a simple and ecological solution to promote plants growth in prospective extraterrestrial human outposts. In this study, we investigated the adaptation of the legume symbiont Paraburkholderia phymatum to simulated microgravity (s0-g) at the transcriptome level by performing an RNA-Seq analysis. The results revealed a drastic effect on gene expression, with roughly 23 % of P. more...

Organism:

Paraburkholderia phymatum STM815

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22728

6 Samples

Download data: TXT

(Submitter supplied) Volatilization of lower-chlorinated polychlorinated biphenyls (LC-PCBs) from sediment poses health threats to nearby communities and ecosystems. Biodegradation combined with black carbon (BC) materials is an emerging approach to remove PCBs from sediment, but development of aerobic biofilms on BC for long-term, sustained LC-PCBs remediation is poorly understood. This work aimed to characterize cell enrichment and activity of biphenyl- and benzoate-grown Paraburkholderia xenovorans strain LB400 on various BCs. more...

Organism:

Paraburkholderia xenovorans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33884

12 Samples

Download data: XLSX

(Submitter supplied) Paraburkholderia phymatum STM815 is a nitrogen-fixing endosymbiont that forms root nodules on the agriculturally important Phaseolus vulgaris and other host plants. We previously showed that the nodules induced by a STM815 mutant of the gene encoding the master regulator of nitrogen fixation NifA showed no nitrogenase activity (Fix-) and increased in number compared to P. vul-garis plants infected with the wild-type strain. more...

Organism:

Paraburkholderia phymatum STM815; Phaseolus vulgaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30237

4 Samples

Download data: TXT

(Submitter supplied) Paraburkholderia phymatum is a beta-proteobacterium, which lives in the soil and is able to enter nitrogen-fixing symbiosis with different legumes. The biological nitrogen fixation (BNF) process is of great ecological and agronomic importance. We previously showed that the expression of the key P. phymatum BNF enzyme – the nitrogenase –is regulated by the sigma factor σ54 (or RpoN) inside root nodules. more...

Organism:

Paraburkholderia phymatum STM815

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22728

6 Samples

Download data: TXT

(Submitter supplied) RpoN (σ54) is the key sigma factor for the regulation of transcription of nitrogen fixation genes in diazotrophic bacteria, which include alpha- and beta-rhizobia. Our previous studies showed that a rpoN mutant of the beta-rhizobial strain Paraburkholderia phymatum formed root nodules on Phaseolus vulgaris that were unable to reduce atmospheric nitrogen into ammonia. In an effort to further characterize the RpoN regulon of P. more...

Organism:

Paraburkholderia phymatum STM815

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22728

4 Samples

Download data: TXT

(Submitter supplied) Paraburkholderia phymatum belongs to the β-subclass of proteobacteria. It has recently been shown to be able to nodulate and fix nitrogen in symbiosis with several mimosoid and papillionoid legumes. In contrast to symbiosis of legumes with α-proteobacteria, very little is known about the molecular determinants underlying the successful establishment of this mutualistic relationship with β-proteobacteria. more...

Organism:

Paraburkholderia phymatum STM815

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22728

9 Samples

Download data: TXT

(Submitter supplied) Burkholderia caribensis MBA4 is able to metabolize monochloroacetate (MCA) as sole carbon and energy source for growth. A haloacid dehalogenase is mainly responsible for the cleavage of the carbon-halogen bond. The MCA will be hydrolyzed to glycolate which would be further transformed to glyoxylate by glycolate oxidase. In order to identify inducible genes involved in mineralization of MCA, transcriptomes of MBA4 cells grown on MCA, glycolate or pyruvate were determined using RNA-seq technology. more...

Organism:

Paraburkholderia caribensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20804 GPL20803

5 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Escherichia coli; Shigella flexneri; Haemophilus influenzae; Staphylococcus aureus; Lactobacillus delbrueckii subsp. bulgaricus; Mycobacterium avium; Human betaherpesvirus 6; human gammaherpesvirus 4; Human T-cell leukemia virus type I; Coxsackievirus A9; Human herpesvirus 4 type 2; Chlamydia pneumoniae; Human poliovirus 3 strain Sabin; Betapolyomavirus macacae; Proteus mirabilis; Streptococcus pyogenes; Mycobacterium gordonae; Trypanosoma cruzi; Caprine arthritis encephalitis virus; Human T-cell lymphotrophic virus type 1 (isolate MT-2); Human adenovirus 12; Human endogenous retrovirus K; Human Endogenous Retrovirus IDDMK1,2-22; Torque teno virus; Prochlorococcus marinus str. MIT 9202; Human rotavirus MP409; Paraburkholderia fungorum; Staphylococcus aureus A9635; Hepacivirus hominis; Klebsiella pneumoniae; Porphyromonas gingivalis; Mycobacterium avium subsp. paratuberculosis; Mycobacterium tuberculosis; Homo sapiens; Mus musculus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Human herpesvirus 4 strain B95-8; Human immunodeficiency virus 1; Human herpesvirus 4 type 1; Coxsackievirus B4 (strain E2); Helicobacter pylori; Yersinia enterocolitica; Chlamydia trachomatis; Mycobacterium tuberculosis variant bovis; Human alphaherpesvirus 2; Hepatitis C virus isolate HC-J6; Coxsackievirus B4; Coxsackievirus A20; Porphyromonas gingivalis W83; Mycobacterium avium subsp. paratuberculosis K-10

Type:

Protein profiling by protein array

Platforms:

GPL19700 GPL19692 GPL19701

29 Samples

Download data: XLSX

(Submitter supplied) The aim of the array was to determine the BXD2 mouse sera IgG and IgM reactivity profile to linear peptide epitopes. Pooled sera from three 6-9 month old BXD2 mice was diluted at 1:200 for IgG specific analysis or 1:1000 for IgM specific analysis and incubated on a PEPperPRINT peptide microarray platform printed with peptide autoantigens

Organism:

Chlamydia trachomatis; Streptococcus pyogenes; Mycobacterium tuberculosis variant bovis; Mycobacterium tuberculosis; Trypanosoma cruzi; Homo sapiens; Human herpesvirus 4 strain B95-8; Human T-cell lymphotrophic virus type 1 (isolate MT-2); Coxsackievirus B4; Human adenovirus 12; Human Endogenous Retrovirus IDDMK1,2-22; Human rotavirus MP409; Hepacivirus hominis; Haemophilus influenzae; Lactobacillus delbrueckii subsp. bulgaricus; Mycobacterium avium; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Coxsackievirus A9; Coxsackievirus B4 (strain E2); Betapolyomavirus macacae; Helicobacter pylori; Shigella flexneri; Yersinia enterocolitica; Human alphaherpesvirus 2; Hepatitis C virus isolate HC-J6; Human T-cell leukemia virus type I; Coxsackievirus A20; Torque teno virus; Prochlorococcus marinus str. MIT 9202; Paraburkholderia fungorum; Porphyromonas gingivalis W83; Mycobacterium avium subsp. paratuberculosis K-10; Staphylococcus aureus A9635; Escherichia coli; Klebsiella pneumoniae; Proteus mirabilis; Porphyromonas gingivalis; Staphylococcus aureus; Mycobacterium avium subsp. paratuberculosis; Mycobacterium gordonae; Mus musculus; Human betaherpesvirus 6; Human immunodeficiency virus 1; Human herpesvirus 4 type 2; Human herpesvirus 4 type 1; Human endogenous retrovirus K; Chlamydia pneumoniae; Human poliovirus 3 strain Sabin

Type:

Protein profiling by protein array

Platform:

GPL19701

2 Samples

Download data: XLSX

(Submitter supplied) The aim of the array was to determine the B6 mouse sera IgG and IgM reactivity profile to linear peptide epitopes. Pooled sera from three 6-9 month old B6 mice was diluted at 1:200 for IgG specific analysis or 1:1000 for IgM specific analysis and incubated on a PEPperPRINT peptide microarray platform printed with peptide autoantigens

Organism:

Chlamydia trachomatis; Streptococcus pyogenes; Mycobacterium tuberculosis variant bovis; Trypanosoma cruzi; Human betaherpesvirus 6; Human T-cell lymphotrophic virus type 1 (isolate MT-2); Coxsackievirus B4; Human adenovirus 12; Human endogenous retrovirus K; Human Endogenous Retrovirus IDDMK1,2-22; Human rotavirus MP409; Hepacivirus hominis; Helicobacter pylori; Proteus mirabilis; Yersinia enterocolitica; Porphyromonas gingivalis; Mycobacterium gordonae; Human alphaherpesvirus 2; Hepatitis C virus isolate HC-J6; Human herpesvirus 4 type 1; Coxsackievirus A20; Escherichia coli; Klebsiella pneumoniae; Shigella flexneri; Staphylococcus aureus; Mycobacterium avium subsp. paratuberculosis; Mus musculus; Human immunodeficiency virus 1; Human herpesvirus 4 type 2; Chlamydia pneumoniae; Human poliovirus 3 strain Sabin; Torque teno virus; Paraburkholderia fungorum; Porphyromonas gingivalis W83; Mycobacterium avium subsp. paratuberculosis K-10; Haemophilus influenzae; Lactobacillus delbrueckii subsp. bulgaricus; Mycobacterium avium; Mycobacterium tuberculosis; Homo sapiens; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Human herpesvirus 4 strain B95-8; Human T-cell leukemia virus type I; Coxsackievirus A9; Prochlorococcus marinus str. MIT 9202; Coxsackievirus B4 (strain E2); Staphylococcus aureus A9635; Betapolyomavirus macacae

Type:

Protein profiling by protein array

Platform:

GPL19701

2 Samples

Download data: XLSX

(Submitter supplied) Despite various efforts to develop tools to detect and compare the activity and catabolic potential and activity for pollutant degradation of microorganisms in environmental samples, an open-source, curated and reliable method is still required. Here we report on a customize normalization system that can be applied to any microarray design, allowing the assessment of reliability of signals and enabling cross-experiment comparisons. more...

Organism:

Rhizorhabdus wittichii RW1; unidentified microorganism; Pseudomonas putida F1; Cupriavidus pinatubonensis JMP134; Paraburkholderia xenovorans LB400

Type:

Expression profiling by array

Platform:

GPL13771

23 Samples

Download data: TXT

(Submitter supplied) comparative genomic hybridization between Burkholderia cepacia sequenced strains to determine what are the limits to strain relativity in order to still provide accurate estimations of gene content

Organism:

Burkholderia cenocepacia; Burkholderia vietnamiensis G4; Burkholderia cenocepacia J2315; Paraburkholderia xenovorans LB400; Burkholderia ambifaria AMMD; Burkholderia cenocepacia HI2424; Burkholderia orbicola

Type:

Genome variation profiling by array

Platform:

GPL9990

12 Samples

Download data: TXT

(Submitter supplied) Laser printer spotted peptides Protocol: Amino acid microparticles are laser printed onto a glass slide with a custom 24-color laser printer. One layer of an amino acid particle pattern is printed on a coated glass slide. A heating step melts the particles. Embedded amino acids can couple to the chip surface. In accordance with solid phase Fmoc chemistry, a cycle of synthesis is completed after removal of excessive material by washing and cleavage of the N-terminal Fmoc protection group. more...

Organism:

Helicobacter pylori; Escherichia coli; Klebsiella pneumoniae; Proteus mirabilis; Shigella flexneri; Yersinia enterocolitica; Haemophilus influenzae; Chlamydia trachomatis; Porphyromonas gingivalis; Staphylococcus aureus; Streptococcus pyogenes; Lactobacillus delbrueckii subsp. bulgaricus; Mycobacterium avium; Mycobacterium tuberculosis variant bovis; Mycobacterium avium subsp. paratuberculosis; Mycobacterium tuberculosis; Mycobacterium gordonae; Trypanosoma cruzi; Homo sapiens; Mus musculus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Human betaherpesvirus 6; human gammaherpesvirus 4; Human herpesvirus 4 strain B95-8; Hepatitis C virus isolate HC-J6; Human immunodeficiency virus 1; Human T-cell leukemia virus type I; Human T-cell lymphotrophic virus type 1 (isolate MT-2); Coxsackievirus A9; Coxsackievirus B4; Human herpesvirus 4 type 2; Human adenovirus 12; Human herpesvirus 4 type 1; Coxsackievirus A20; Human endogenous retrovirus K; Human Endogenous Retrovirus IDDMK1,2-22; Torque teno virus; Chlamydia pneumoniae; Prochlorococcus marinus str. MIT 9202; Human rotavirus MP409; Coxsackievirus B4 (strain E2); Paraburkholderia fungorum; Porphyromonas gingivalis W83; Mycobacterium avium subsp. paratuberculosis K-10; Human poliovirus 3 strain Sabin; Staphylococcus aureus A9635; Betapolyomavirus macacae; Hepacivirus hominis

3 Series

4 Samples

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