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Phactr4 phosphatase and actin regulator 4 [ Mus musculus (house mouse) ]

Gene ID: 100169, updated on 9-Dec-2024

Summary

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Official Symbol
Phactr4provided by MGI
Official Full Name
phosphatase and actin regulator 4provided by MGI
Primary source
MGI:MGI:2140327
See related
Ensembl:ENSMUSG00000066043 AllianceGenome:MGI:2140327
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
mKIAA4120; 3110001B12Rik; C330013F19Rik
Summary
Enables actin binding activity; protein phosphatase 1 binding activity; and protein phosphatase activator activity. Involved in several processes, including enteric nervous system development; morphogenesis of embryonic epithelium; and negative regulation of integrin-mediated signaling pathway. Located in lamellipodium. Is expressed in several structures, including 1st branchial arch mandibular component; central nervous system; facial prominence; and retina. Orthologous to human PHACTR4 (phosphatase and actin regulator 4). [provided by Alliance of Genome Resources, Dec 2024]
Expression
Ubiquitous expression in CNS E11.5 (RPKM 6.0), limb E14.5 (RPKM 5.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Phactr4 in Genome Data Viewer
Location:
4 D2.3; 4 65.24 cM
Exon count:
17
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (132083233..132149765, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (132355922..132422446, complement)

Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene CRISPRi-validated cis-regulatory element for Atpif1, Phactr4, Ptpru, Rcc1 and Snhg3 Neighboring gene small nucleolar RNA, H/ACA box 73b Neighboring gene small nucleolar RNA, H/ACA box 73a Neighboring gene small nucleolar RNA host gene 3 Neighboring gene predicted gene, 23055 Neighboring gene STARR-seq mESC enhancer starr_11566 Neighboring gene STARR-positive B cell enhancer ABC_E9596 Neighboring gene mediator complex subunit 18

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool. Huet G, et al. J Cell Sci, 2013 Jan 15. PMID 23203801
  2. A role for natural regulatory T cells in the pathogenesis of experimental cerebral malaria. Amante FH, et al. Am J Pathol, 2007 Aug. PMID 17600128, Free PMC Article
  3. Estrogen receptor beta increases clear cell renal cell carcinoma stem cell phenotype via altering the circPHACTR4/miR-34b-5p/c-Myc signaling. Wang Y, et al. FASEB J, 2022 Feb. PMID 35061326
  4. Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control. Patel VL, et al. Genes Dev, 2012 Jan 1. PMID 22215810, Free PMC Article
  5. Phosphatase and actin regulator 4 is associated with intermediate filaments in adult neural stem cells and their progenitor astrocytes. Cho HM, et al. Histochem Cell Biol, 2014 Oct. PMID 24748504

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Estrogen receptor beta increases clear cell renal cell carcinoma stem cell phenotype via altering the circPHACTR4/miR-34b-5p/c-Myc signaling.
    Title: Estrogen receptor beta increases clear cell renal cell carcinoma stem cell phenotype via altering the circPHACTR4/miR-34b-5p/c-Myc signaling.
  2. The Phactr4 forms functional associations with intermediate filament networks in adult neural stem cells.
    Title: Phosphatase and actin regulator 4 is associated with intermediate filaments in adult neural stem cells and their progenitor astrocytes.
  3. Data discloses the central role of Phactr4 in a feedback loop, where actin monomers regulate their own number via the activation of a key regulator of actin dynamics.
    Title: Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool.
  4. This study demonistrated that the mRNA expression of phactr4 was progressively increased in the injury penumbra.
    Title: Different expression patterns of Phactr family members in normal and injured mouse brain.
  5. hactr4 negatively regulates integrin signaling through the RHO/ROCK pathway and coordinates protein phosphatase 1 (PP1) with cofilin activity to regulate cytoskeletal dynamics
    Title: Phactr4 regulates directional migration of enteric neural crest through PP1, integrin signaling, and cofilin activity.
  6. Phactr4 is critical for the spatially and temporally regulated transition in proliferation through differential regulation of PP1 and the cell cycle during neurulation and eye development
    Title: Phactr4 regulates neural tube and optic fissure closure by controlling PP1-, Rb-, and E2F1-regulated cell-cycle progression.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • FLJ13171, KIAA4120

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables actin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables actin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein phosphatase 1 binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein phosphatase activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein phosphatase activator activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein phosphatase inhibitor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in Rho protein signal transduction IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin cytoskeleton organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in actin cytoskeleton organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in closure of optic fissure IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in enteric nervous system development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of integrin-mediated signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neural crest cell migration IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in neural crest cell migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neural tube closure IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of catalytic activity IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in lamellipodium IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
phosphatase and actin regulator 4
Names
humdy
protein Humpty dumpty

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001161797.2 → NP_001155269.1  phosphatase and actin regulator 4 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL805897
    Consensus CDS
    CCDS51318.1
    UniProtKB/Swiss-Prot
    Q501J7
    Related
    ENSMUSP00000081185.6, ENSMUST00000084170.12
    Conserved Domains (1) summary
    smart00707
    Location:549 → 573
    RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2

  2. NM_175306.5 → NP_780515.2  phosphatase and actin regulator 4 isoform 1

    See identical proteins and their annotated locations for NP_780515.2

    Status: VALIDATED

    Source sequence(s)
    AL805897
    Consensus CDS
    CCDS18724.1
    UniProtKB/Swiss-Prot
    A2ALF7, A2ALG0, F6QNZ3, Q501J7, Q5DTM4, Q6DI97, Q8C7U9
    Related
    ENSMUSP00000099628.4, ENSMUST00000102568.10
    Conserved Domains (2) summary
    smart00707
    Location:576 → 600
    RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2
    pfam02755
    Location:64 → 86
    RPEL; RPEL repeat

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000070.7 Reference GRCm39 C57BL/6J

    Range
    132083233..132149765 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036163538.1 → XP_036019431.1  phosphatase and actin regulator 4 isoform X3

    Conserved Domains (1) summary
    smart00707
    Location:491 → 515
    RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2

  2. XM_006538456.5 → XP_006538519.1  phosphatase and actin regulator 4 isoform X1

    UniProtKB/Swiss-Prot
    Q501J7
    Related
    ENSMUSP00000081270.5, ENSMUST00000084249.11
    Conserved Domains (2) summary
    smart00707
    Location:586 → 610
    RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2
    pfam02755
    Location:75 → 97
    RPEL; RPEL repeat

  3. XM_006538457.5 → XP_006538520.1  phosphatase and actin regulator 4 isoform X2

    Conserved Domains (1) summary
    smart00707
    Location:559 → 583
    RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2

  4. XM_011250172.2 → XP_011248474.1  phosphatase and actin regulator 4 isoform X5

    Conserved Domains (1) summary
    smart00707
    Location:302 → 326
    RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2

  5. XM_036163539.1 → XP_036019432.1  phosphatase and actin regulator 4 isoform X4

    Conserved Domains (1) summary
    pfam02755
    Location:75 → 97
    RPEL; RPEL repeat
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q501J7.2 GenPept UniProtKB/Swiss-Prot:Q501J7

Gene LinkOut

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