Endog endonuclease G [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Endogprovided by MGI
Official Full Name: endonuclease Gprovided by MGI
Primary source: MGI:MGI:1261433
See related: Ensembl:ENSMUSG00000015337 AllianceGenome:MGI:1261433
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables DNA endonuclease activity; magnesium ion binding activity; and protein homodimerization activity. Involved in negative regulation of TOR signaling and positive regulation of autophagy. Acts upstream of or within several processes, including apoptotic DNA fragmentation; in utero embryonic development; and response to tumor necrosis factor. Located in mitochondrion. Is expressed in several structures, including brown fat; gut; liver; male reproductive gland or organ; and respiratory system. Orthologous to human ENDOG (endonuclease G). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in ovary adult (RPKM 57.2), duodenum adult (RPKM 56.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 B; 2 21.29 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (30061525..30064057)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (30171408..30174069)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Caspase-3 promotes oncogene-induced malignant transformation via EndoG-dependent Src-STAT3 phosphorylation.
Title: Caspase-3 promotes oncogene-induced malignant transformation via EndoG-dependent Src-STAT3 phosphorylation.
Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress.
Title: Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress.
Crystal structure of the mouse endonuclease G.
Title: Crystal structure of the mouse endonuclease G.
The specificity of endonuclease G for 5-hydroxymethylcytosine (5hmC) and Holliday junctions derives from structural adaptations that distinguish the vertebrate from the invertebrate enzyme, thereby thereby supporting a role for 5hmC in recombination processes.
Title: Structural adaptation of vertebrate endonuclease G for 5-hydroxymethylcytosine recognition and function.
These results support the involvement of redox signaling in the control of cardiomyocyte growth by ENDOG and suggest a pathway relating mtDNA content to the regulation of cell growth probably involving humanin, which prevents reactive oxygen radicals accumulation and hypertrophy induced by Endog deficiency.
Title: Cardiomyocyte hypertrophy induced by Endonuclease G deficiency requires reactive oxygen radicals accumulation and is inhibitable by the micropeptide humanin.
Deficiency of AKT2 in myocardium results in diminished MEF2A abundance, which induced decreased size of cardiomyocytes. We additionally confirmed that EndoG, which is also regulated by AKT2, is a suppressor of MEF2A in myocardium.
Title: AKT2 deficiency induces retardation of myocyte development through EndoG-MEF2A signaling in mouse heart.
increased browning of white adipose tissue elicited by the lack of EndoG was associated with a better glucose tolerance and reduced fat mass
Title: EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis.
This study demonistated that Endonuclease G knockout mice reveals a new putative molecular player in the regulation of anxiety.
Title: Neurobehavioral characterization of Endonuclease G knockout mice reveals a new putative molecular player in the regulation of anxiety.
These findings determine a role for EndoG in the generation of Switch region double-strand DNA breaks and class switch DNA recombination.
Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions.
Data demonstrate the importance of DNase I and EndoG in host cell defense against gene and RNA delivery to renal tubular epithelial cells in vitro.
Title: Uptake of foreign nucleic acids in kidney tubular epithelial cells deficient in proapoptotic endonucleases.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BI278171 (e-PCR)
- UniSTS:244249

RH124237 (e-PCR)
- UniSTS:161059

D2Wsu81e (e-PCR), detects polymorphism
- UniSTS:142378

RH124858 (e-PCR)
- UniSTS:161680

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA endonuclease activity	ISO Inferred from Sequence Orthology more info
enables DNA nuclease activity	ISO Inferred from Sequence Orthology more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables single-stranded DNA endodeoxyribonuclease activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA catabolic process	TAS Traceable Author Statement more info	PubMed
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
involved_in apoptotic DNA fragmentation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within apoptotic DNA fragmentation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to calcium ion	IEA Inferred from Electronic Annotation more info
involved_in cellular response to glucose stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to hypoxia	IEA Inferred from Electronic Annotation more info
involved_in cellular response to oxidative stress	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial DNA catabolic process	ISO Inferred from Sequence Orthology more info
involved_in mitochondrial DNA catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of TOR signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TOR signaling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic DNA fragmentation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of apoptotic DNA fragmentation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of hydrogen peroxide-mediated programmed cell death	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of hydrogen peroxide-mediated programmed cell death	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitochondrial DNA replication	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitochondrial DNA replication	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within response to antibiotic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to estradiol	IEA Inferred from Electronic Annotation more info
involved_in response to mechanical stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to tumor necrosis factor	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in mitochondrial inner membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in perikaryon	IEA Inferred from Electronic Annotation more info
located_in perikaryon	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: endonuclease G, mitochondrial

Names: endo G

NP_031957.1

EC 3.1.30.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007931.2 → NP_031957.1 endonuclease G, mitochondrial precursor

See identical proteins and their annotated locations for NP_031957.1

Status: VALIDATED

Source sequence(s)

AL845258

Consensus CDS

CCDS15872.1

UniProtKB/Swiss-Prot

O08600

UniProtKB/TrEMBL

Q3UN47

Related

ENSMUSP00000015481.6, ENSMUST00000015481.6

Conserved Domains (2) summary

smart00477
Location:75 → 281

NUC; DNA/RNA non-specific endonuclease

COG1864
Location:29 → 278

NUC1; DNA/RNA endonuclease G, NUC1 [Nucleotide transport and metabolism]