Kcnu1 potassium channel, subfamily U, member 1 [Mus musculus (house mouse)] - Gene

Summary

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Official Symbol: Kcnu1provided by MGI
Official Full Name: potassium channel, subfamily U, member 1provided by MGI
Primary source: MGI:MGI:1202300
See related: Ensembl:ENSMUSG00000031576 AllianceGenome:MGI:1202300
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Slo3; mSlo3; Kcnma3
Summary: Enables potassium channel activity. Involved in fertilization and potassium ion transport. Acts upstream of or within establishment of localization in cell and protein stabilization. Located in plasma membrane. Is expressed in germ cell of testis. Human ortholog(s) of this gene implicated in spermatogenic failure 79. Orthologous to human KCNU1 (potassium calcium-activated channel subfamily U member 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in testis adult (RPKM 17.3) and CNS E18 (RPKM 1.2) See more
Orthologs: human all
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Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (26338656..26427971)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (25848627..25937943)

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transmembrane determinants of voltage-gating differences between BK (Slo1) and Slo3 channels.
Title: Transmembrane determinants of voltage-gating differences between BK (Slo1) and Slo3 channels.
Study confirms that expression of full-length Slo3 is sperm-specific but warns against developing contraceptive drugs targeting the C-terminal tail of Slo3 channels.
Title: A cytoplasmic Slo3 isoform is expressed in somatic tissues.
Considering that capacitation-induced hyperpolarization is mediated by SLO3, we evaluated the action of cSrc inhibitors on the heterologously expressed SLO3 channel.
Title: Src Kinase Is the Connecting Player between Protein Kinase A (PKA) Activation and Hyperpolarization through SLO3 Potassium Channel Regulation in Mouse Sperm.
These results establish a critical role of LRRC52 in KSPER channels and demonstrate that loss of a non-pore-forming auxiliary subunit results in severe fertility impairment.
Title: SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER currents and is critical for normal fertility.
SLO3 K+ channels have a role in controlling calcium entry through CATSPER channels in sperm
Title: SLO3 K+ channels control calcium entry through CATSPER channels in sperm.
Using mice lacking both SLO3 and CATSPER1 subunits, the results show conclusively that the voltage-activated outward current present in Slo3 (-/-) sperm is abolished when CATSPER is also deleted.
Title: Simultaneous knockout of Slo3 and CatSper1 abolishes all alkalization- and voltage-activated current in mouse spermatozoa.
SLO3-dependent hyperpolarization
Title: Ion permeabilities in mouse sperm reveal an external trigger for SLO3-dependent hyperpolarization.
KSper/Slo3 is the primary spermatozoan K(+) channel, and Slo3 is critical for fertility.
Title: Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa.
Phosphatidylinositol 4,5-bisphosphate activates Slo3 currents and its hydrolysis underlies the epidermal growth factor-induced current inhibition
Title: Phosphatidylinositol 4,5-bisphosphate activates Slo3 currents and its hydrolysis underlies the epidermal growth factor-induced current inhibition.
Compare pharmacological properties of the pH-regulated Slo3 channel and its close homologue, the Slo1 or BK channel.
Title: Block of mouse Slo1 and Slo3 K+ channels by CTX, IbTX, TEA, 4-AP and quinidine.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
NOT enables large conductance calcium-activated potassium channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables potassium channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables potassium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables potassium channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables potassium channel activity	ISO Inferred from Sequence Orthology more info
enables potassium channel activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in potassium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in potassium ion transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within potassium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within reproductive process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reproductive process	ISO Inferred from Sequence Orthology more info
involved_in reproductive process	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
part_of monoatomic ion channel complex	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in sperm flagellum	ISO Inferred from Sequence Orthology more info
located_in sperm flagellum	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: potassium channel subfamily U member 1

Names: KSper; calcium-activated potassium channel subunit alpha-3; calcium-activated potassium channel, subfamily M subunit alpha-3; pH-sensitive maxi potassium channel; pore-forming subunit of the sperm-specific alkalization activated K(+) current; potassium large conductance pH-sensitive channel, subfamily M, alpha member 3; slowpoke homolog 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008432.3 → NP_032458.3 potassium channel subfamily U member 1

See identical proteins and their annotated locations for NP_032458.3

Status: VALIDATED

Source sequence(s)

AF039213, BQ175543, CJ042506

Consensus CDS

CCDS52530.1

UniProtKB/Swiss-Prot

D3Z5P2, G3X9P7, O54982

Related

ENSMUSP00000096457.4, ENSMUST00000098858.11

Conserved Domains (3) summary

pfam00520
Location:133 → 323

Ion_trans; Ion transport protein

pfam03493
Location:469 → 557

BK_channel_a; Calcium-activated BK potassium channel alpha subunit

pfam07885
Location:230 → 316

Ion_trans_2; Ion channel

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

26338656..26427971

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017312583.2 → XP_017168072.1 potassium channel subfamily U member 1 isoform X4

Conserved Domains (2) summary

pfam00520
Location:12 → 241

Ion_trans; Ion transport protein

pfam03493
Location:387 → 475

BK_channel_a; Calcium-activated BK potassium channel alpha subunit
XM_006509024.2 → XP_006509087.1 potassium channel subfamily U member 1 isoform X1

Conserved Domains (2) summary

pfam00520
Location:104 → 333

Ion_trans; Ion transport protein

pfam03493
Location:479 → 567

BK_channel_a; Calcium-activated BK potassium channel alpha subunit
XM_017312582.1 → XP_017168071.1 potassium channel subfamily U member 1 isoform X3

Conserved Domains (2) summary

pfam00520
Location:104 → 333

Ion_trans; Ion transport protein

pfam03493
Location:479 → 567

BK_channel_a; Calcium-activated BK potassium channel alpha subunit
XM_017312581.1 → XP_017168070.1 potassium channel subfamily U member 1 isoform X2

Conserved Domains (2) summary

pfam00520
Location:104 → 333

Ion_trans; Ion transport protein

pfam03493
Location:479 → 567

BK_channel_a; Calcium-activated BK potassium channel alpha subunit