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Tpst2 protein-tyrosine sulfotransferase 2 [ Mus musculus (house mouse) ]

Gene ID: 22022, updated on 9-Dec-2024

Summary

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Official Symbol
Tpst2provided by MGI
Official Full Name
protein-tyrosine sulfotransferase 2provided by MGI
Primary source
MGI:MGI:1309516
See related
Ensembl:ENSMUSG00000029344 AllianceGenome:MGI:1309516
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
grm; grt; D5Ucla3; Tango13b
Summary
Enables protein-tyrosine sulfotransferase activity. Involved in peptidyl-tyrosine sulfation. Acts upstream of or within fusion of sperm to egg plasma membrane involved in single fertilization and prevention of polyspermy. Is active in Golgi lumen and trans-Golgi network. Orthologous to human TPST2 (tyrosylprotein sulfotransferase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression
Broad expression in genital fat pad adult (RPKM 79.3), adrenal adult (RPKM 73.7) and 25 other tissues See more
Orthologs
human all
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Genomic context

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See Tpst2 in Genome Data Viewer
Location:
5 F; 5 54.65 cM
Exon count:
10
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (112424548..112463222)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (112276682..112315356)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene crystallin, beta A4 Neighboring gene STARR-positive B cell enhancer ABC_E6365 Neighboring gene crystallin, beta B1 Neighboring gene STARR-positive B cell enhancer ABC_E6366 Neighboring gene STARR-positive B cell enhancer ABC_E1685 Neighboring gene predicted gene, 46941 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:112714594-112714795 Neighboring gene STARR-positive B cell enhancer ABC_E3600 Neighboring gene tuftelin interacting protein 11 Neighboring gene SRR1 domain containing Neighboring gene coiled-coil domain containing 121, retrogene 3 Neighboring gene HPS4, biogenesis of lysosomal organelles complex 3 subunit 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Identifying quantitative trait loci affecting resistance to congenital hypothyroidism in 129/SvJcl strain mice. Hosoda Y, et al. PLoS One, 2012. PMID 22299049, Free PMC Article
  2. Impaired insulin secretion from the pancreatic islets of hypothyroidal growth-retarded mice. Taguchi Y, et al. J Endocrinol, 2010 Aug. PMID 20488945
  3. Relationship between growth retardation and impaired glucose tolerance in hypothyroidal growth-retarded (grt) mice. Tasaki Y, et al. Congenit Anom (Kyoto), 2010 Sep. PMID 20608948
  4. Hypothyroid phenotype of the Tpst2 mutant mouse is dependent upon genetic background. Hosoda Y, et al. Biomed Res, 2010 Jun. PMID 20622471
  5. Testicular development in growth-retarded mice. Kobayashi K, et al. Exp Anim, 2007 Oct. PMID 18075202

See all (55) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. TPST2-mediated receptor tyrosine sulfation enhances leukocidin cytotoxicity and S. aureus infection.
    Title: TPST2-mediated receptor tyrosine sulfation enhances leukocidin cytotoxicity and S. aureus infection.
  2. cerebral abnormalities in grt/grt Tpst2 gene defect mice are characterized by cortical hypoplasia at the frontooccipital axis with immature pyramidal neurons and insufficient development of callosal fibers
    Title: Cerebral cortical hypoplasia with abnormal morphology of pyramidal neuron in growth-retarded mouse (grt/grt).
  3. Findings indicate that low body weight and salivary gland hypofunction in Tpst2-/- mice is due solely to primary hypothyroidism.
    Title: Salivary gland hypofunction in tyrosylprotein sulfotransferase-2 knockout mice is due to primary hypothyroidism.
  4. Results demonstrate the importance of protein-tyrosine sulfation for proper development of the retina and suggest different phenotypes resulting from elimination of TPST-1 or -2 may reflect differential expression patterns or levels of the enzymes.
    Title: Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2.
  5. Data show that Tpst1/Tpst2 DKO leukocytes bound less P-selectin than wild type leukocytes despite equivalent surface expression of Psgl-1.
    Title: Tyrosine sulfation of native mouse Psgl-1 is required for optimal leukocyte rolling on P-selectin in vivo.
  6. Lack of tyrosylprotein sulfotransferase-2 activity results in altered sperm-egg interactions and loss of ADAM3 and ADAM6 in epididymal sperm.
    Title: Lack of tyrosylprotein sulfotransferase-2 activity results in altered sperm-egg interactions and loss of ADAM3 and ADAM6 in epididymal sperm.
  7. These results indicate that protein-tyrosine sulfation by Tpst1/2 is essential for proper outer segment morphogenesis and synaptic function, but is not critical for overall retinal structure or synapse formation.
    Title: Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy.
  8. demonstrated that the Tpst2 transgene rescues the mutant phenotypes both in vitro and in vivo
    Title: Hypothyroid phenotype of the Tpst2 mutant mouse is dependent upon genetic background.
  9. Considering that Tpst2 is the responsible gene of grt mice, mutation of which is associated with a poor function of TSH receptor, the findings raise the possibility of involvement of factors including Tpst2 in the insulin hyposecretion in grt mice.
    Title: Impaired insulin secretion from the pancreatic islets of hypothyroidal growth-retarded mice.
  10. demonstrate that RNase 9 and Mfge8 are tyrosine-sulfated in wild type and Tpst1(-/-), but not in Tpst2(-/-) mice. This suggests lack of sulfation of one or both of these proteins may contribute mechanistically to the infertility of Tpst2(-/-) males
    Title: Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo.
Submit: New GeneRIF Correction See all GeneRIFs (13)

Phenotypes

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Associated conditions

Description Tests
growth retarded
GeneReviews: Not available

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables protein-tyrosine sulfotransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-tyrosine sulfotransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-tyrosine sulfotransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein-tyrosine sulfotransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein-tyrosine sulfotransferase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within fusion of sperm to egg plasma membrane involved in single fertilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in peptidyl-tyrosine sulfation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within peptidyl-tyrosine sulfation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in peptidyl-tyrosine sulfation ISO
Inferred from Sequence Orthology
more info
  
involved_in peptidyl-tyrosine sulfation ISS
Inferred from Sequence or Structural Similarity
more info
  
acts_upstream_of_or_within prevention of polyspermy IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in Golgi apparatus IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in Golgi lumen IDA
Inferred from Direct Assay
more info
 PubMed 
located_in Golgi membrane IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum ISO
Inferred from Sequence Orthology
more info
  
is_active_in trans-Golgi network IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in trans-Golgi network ISO
Inferred from Sequence Orthology
more info
 PubMed 

General protein information

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Preferred Names
protein-tyrosine sulfotransferase 2
Names
TPST-2
tyrosylprotein sulfotransferase 2
NP_001313519.2
NP_001361637.1
NP_001361638.1
NP_033445.3
XP_006534912.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001326590.2 → NP_001313519.2  protein-tyrosine sulfotransferase 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 4. Variants 1-4 all encode the same protein.
    Source sequence(s)
    AC123795, AC123848
    UniProtKB/Swiss-Prot
    O88856, Q8CBA8
    UniProtKB/TrEMBL
    Q3UDH0
    Related
    ENSMUSP00000071399.3, ENSMUST00000071455.7
    Conserved Domains (1) summary
    pfam13469
    Location:70 → 261
    Sulfotransfer_3; Sulfotransferase family

  2. NM_001374708.1 → NP_001361637.1  protein-tyrosine sulfotransferase 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 4. Variants 1-4 all encode the same protein.
    Source sequence(s)
    AC123848
    UniProtKB/Swiss-Prot
    O88856, Q8CBA8
    UniProtKB/TrEMBL
    Q3UDH0
    Conserved Domains (1) summary
    pfam13469
    Location:70 → 261
    Sulfotransfer_3; Sulfotransferase family

  3. NM_001374709.1 → NP_001361638.1  protein-tyrosine sulfotransferase 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) represents the longest transcript. Variants 1-4 all encode the same protein.
    Source sequence(s)
    AC123848
    UniProtKB/Swiss-Prot
    O88856, Q8CBA8
    UniProtKB/TrEMBL
    Q3UDH0
    Conserved Domains (1) summary
    pfam13469
    Location:70 → 261
    Sulfotransfer_3; Sulfotransferase family

  4. NM_009419.5 → NP_033445.3  protein-tyrosine sulfotransferase 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. Variants 1-4 all encode the same protein.
    Source sequence(s)
    AC123795, AC123848
    UniProtKB/Swiss-Prot
    O88856, Q8CBA8
    UniProtKB/TrEMBL
    Q3UDH0
    Related
    ENSMUSP00000031287.6, ENSMUST00000031287.12
    Conserved Domains (1) summary
    pfam13469
    Location:70 → 261
    Sulfotransfer_3; Sulfotransferase family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    112424548..112463222
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006534849.5 → XP_006534912.1  protein-tyrosine sulfotransferase 2 isoform X1

    UniProtKB/TrEMBL
    Q3UDH0
    Conserved Domains (1) summary
    pfam13469
    Location:135 → 326
    Sulfotransfer_3; Sulfotransferase family

RNA

  1. XR_389265.3 RNA Sequence

  2. XR_003955618.2 RNA Sequence

  3. XR_003955617.2 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O88856.1 GenPept UniProtKB/Swiss-Prot:O88856

Gene LinkOut

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