Cryab crystallin, alpha B [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cryabprovided by RGD
Official Full Name: crystallin, alpha Bprovided by RGD
Primary source: RGD:2414
See related: EnsemblRapid:ENSRNOG00000010524 AllianceGenome:RGD:2414
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: AACRYA
Summary: This gene encodes subunit b, one of two subunits of alpha-crystallin, which is a high molecular weight, soluble aggregate and is a member of the small heat shock protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. It acts as a molecular chaperone and is the major protein in the eye lens, maintaining the transparency and refractive index of the lens. Alternate promoter usage results in different transcript variants encoding the same protein. [provided by RefSeq, Sep 2014]
Expression: Biased expression in Heart (RPKM 17469.9), Muscle (RPKM 7981.4) and 2 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q23

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (59989885..59995532)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (51093441..51099161)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (55178543..55182546)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulation of rat HspB5/alphaB-Crystallin by microRNAs miR-101a-3p, miR-140-5p, miR-330-5p, and miR-376b-3p.
Title: Regulation of rat HspB5/alphaB-Crystallin by microRNAs miR-101a-3p, miR-140-5p, miR-330-5p, and miR-376b-3p.
HspB5/alphaB-crystallin phosphorylation at S45 and S59 is essential for protection of the dendritic tree of rat hippocampal neurons.
Title: HspB5/αB-crystallin phosphorylation at S45 and S59 is essential for protection of the dendritic tree of rat hippocampal neurons.
The transcription factor CREB acts as an important regulator mediating oxidative stress-induced apoptosis by suppressing alphaB-crystallin expression.
Title: The transcription factor CREB acts as an important regulator mediating oxidative stress-induced apoptosis by suppressing αB-crystallin expression.
Alpha B-Crystallin Overexpression Protects Oligodendrocyte Precursor Cells Against Oxidative Stress-Induced Apoptosis Through the Akt Pathway.
Title: Alpha B-Crystallin Overexpression Protects Oligodendrocyte Precursor Cells Against Oxidative Stress-Induced Apoptosis Through the Akt Pathway.
Evidence of the involvement of spinal alphaB-crystallin in the maintenance of bone cancer pain in rats.
Title: Evidence of the involvement of spinal αB-crystallin in the maintenance of bone cancer pain in rats.
overexpression of CRYAB significantly increases the heat resistance of H9C2 cardiomyocytes.
Title: CRYAB protects cardiomyocytes against heat stress by preventing caspase-mediated apoptosis and reducing F-actin aggregation.
A report of a novel 44 kDa protein which is most likely a covalently linked HspB5 dimer.
Title: Small heat shock protein speciation: novel non-canonical 44 kDa HspB5-related protein species in rat and human tissues.
these findings highlighted a novel role of sub-toxic concentrations of human amylin in promoting the secretion of proteic factors by endothelial cells (HspB5 and VEGF) that support the survival and proliferation of neuron-like cells.
Title: Sub-Toxic Human Amylin Fragment Concentrations Promote the Survival and Proliferation of SH-SY5Y Cells via the Release of VEGF and HspB5 from Endothelial RBE4 Cells.
CryaB expression in themyocardium increases in aged rats. Manifestation of cardiomyopathy occurs against the background of increased phosphorylation of CryaB (on Ser59) and its translocation to striated sarcomeres.
Title: Phosphorylation of αB-crystallin in the myocardium: Analysis of relations with aging and cardiomyopathy.
These data suggest that among all small heat shock proteins, HspB1 and alpha(B)-crystallin might be most important in the neuronal stress response to ischemia/reperfusion injury in the brain since their protein levels were upregulated to the highest extent after transient middle cerebral artery occlusion.
Title: Upregulation and phosphorylation of HspB1/Hsp25 and HspB5/αB-crystallin after transient middle cerebral artery occlusion in rats.

Submit: New GeneRIF Correction See all GeneRIFs (52)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC123035P1 (e-PCR)
- UniSTS:270427

Cryab (e-PCR), detects polymorphism
- UniSTS:256365

RH143847 (e-PCR)
- UniSTS:233093

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables amyloid-beta binding	ISO Inferred from Sequence Orthology more info
enables cytoskeletal protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables structural constituent of eye lens	TAS Traceable Author Statement more info	PubMed
enables structural molecule activity	ISO Inferred from Sequence Orthology more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within apoptotic process involved in morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within camera-type eye development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to gamma radiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lens development in camera-type eye	ISO Inferred from Sequence Orthology more info
involved_in microtubule polymerization or depolymerization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within muscle organ development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of amyloid fibril formation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell growth	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of cysteine-type endopeptidase activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of intracellular transport	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein-containing complex assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of reactive oxygen species metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein folding	IDA Inferred from Direct Assay more info	PubMed
involved_in protein refolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of programmed cell death	ISO Inferred from Sequence Orthology more info
involved_in response to estradiol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to heat	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to hydrogen peroxide	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to hydrogen peroxide	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in stress-activated MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within tubulin complex assembly	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in I band	IDA Inferred from Direct Assay more info	PubMed
located_in M band	IDA Inferred from Direct Assay more info	PubMed
located_in Z disc	ISO Inferred from Sequence Orthology more info
located_in actin filament bundle	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in cardiac myofibril	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in contractile muscle fiber	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in lysosome	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
located_in perikaryon	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISS Inferred from Sequence or Structural Similarity more info
located_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: alpha-crystallin B chain

Names: Crystallin, alpha polypeptide 2; alpha(B)-crystallin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012935.4 → NP_037067.1 alpha-crystallin B chain

See identical proteins and their annotated locations for NP_037067.1

Status: REVIEWED

Source sequence(s)

CK357656, M55534

UniProtKB/Swiss-Prot

P23928, Q6LDR2

UniProtKB/TrEMBL

A6J4F4

Conserved Domains (2) summary

cd06498
Location:67 → 150

ACD_alphaB-crystallin_HspB5; Alpha-crystallin domain found in the small heat shock protein (sHsp) alphaB-crystallin (HspB5, 20kDa). sHsps are molecular chaperones that suppress protein aggregation and protect against cell stress, and are generally active as large oligomers ...

pfam00525
Location:1 → 52

Crystallin; Alpha crystallin A chain, N terminal

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

59989885..59995532

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039080879.2 → XP_038936807.1 alpha-crystallin B chain isoform X1

UniProtKB/Swiss-Prot

P23928, Q6LDR2

UniProtKB/TrEMBL

A6J4F4

Related

ENSRNOP00000055901.2, ENSRNOT00000059127.5

Conserved Domains (2) summary

cd06498
Location:67 → 150

ACD_alphaB-crystallin_HspB5; Alpha-crystallin domain found in the small heat shock protein (sHsp) alphaB-crystallin (HspB5, 20kDa). sHsps are molecular chaperones that suppress protein aggregation and protect against cell stress, and are generally active as large oligomers ...

pfam00525
Location:1 → 52

Crystallin; Alpha crystallin A chain, N terminal
XM_039080878.2 → XP_038936806.1 alpha-crystallin B chain isoform X1

UniProtKB/Swiss-Prot

P23928, Q6LDR2

UniProtKB/TrEMBL

A6J4F4

Conserved Domains (2) summary

cd06498
Location:67 → 150

ACD_alphaB-crystallin_HspB5; Alpha-crystallin domain found in the small heat shock protein (sHsp) alphaB-crystallin (HspB5, 20kDa). sHsps are molecular chaperones that suppress protein aggregation and protect against cell stress, and are generally active as large oligomers ...

pfam00525
Location:1 → 52

Crystallin; Alpha crystallin A chain, N terminal