Nat8l N-acetyltransferase 8-like [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nat8lprovided by MGI
Official Full Name: N-acetyltransferase 8-likeprovided by MGI
Primary source: MGI:MGI:2447776
See related: Ensembl:ENSMUSG00000048142 AllianceGenome:MGI:2447776
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Shati; 1110038O08Rik
Summary: Enables aspartate N-acetyltransferase activity. Involved in positive regulation of dopamine uptake involved in synaptic transmission. Located in endoplasmic reticulum membrane. Is expressed in several structures, including central nervous system; hemolymphoid system gland; intestine; lung; and retina. Orthologous to human NAT8L (N-acetyltransferase 8 like). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in cerebellum adult (RPKM 48.7), cortex adult (RPKM 39.5) and 11 other tissues See more
Orthologs: human all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 5 B2; 5 17.83 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (34153328..34163260)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (33995984..34005916)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hindering NAT8L expression in hepatocellular carcinoma increases cytosolic aspartate delivery that fosters pentose phosphate pathway and purine biosynthesis promoting cell proliferation.
Title: Hindering NAT8L expression in hepatocellular carcinoma increases cytosolic aspartate delivery that fosters pentose phosphate pathway and purine biosynthesis promoting cell proliferation.
N-Acetyl Transferase, Shati/Nat8l, in the Dorsal Hippocampus Suppresses Aging-induced Impairment of Cognitive Function in Mice.
Title: N-Acetyl Transferase, Shati/Nat8l, in the Dorsal Hippocampus Suppresses Aging-induced Impairment of Cognitive Function in Mice.
Inhibitory effects of Shati/Nat8l overexpression in the medial prefrontal cortex on methamphetamine-induced conditioned place preference in mice.
Title: Inhibitory effects of Shati/Nat8l overexpression in the medial prefrontal cortex on methamphetamine-induced conditioned place preference in mice.
Shati/Nat8l deficiency disrupts adult neurogenesis and causes attentional impairment through dopaminergic neuronal dysfunction in the dentate gyrus.
Title: Shati/Nat8l deficiency disrupts adult neurogenesis and causes attentional impairment through dopaminergic neuronal dysfunction in the dentate gyrus.
The findings of this study indicated that the striatal expression of N-acetylaspartate synthetase Shati/Nat8l plays a role in major depressive disorder via the metabotropic glutamate receptor 3-mediated functional control of the serotonergic neuronal system.
Title: Striatal N-Acetylaspartate Synthetase Shati/Nat8l Regulates Depression-Like Behaviors via mGluR3-Mediated Serotonergic Suppression in Mice.
Results showed that the Shati/Nat8L mRNA was increased by methamphetamine-induced CREB pathway via dopamine D1 receptor signaling in mouse nucleus accumbens. These findings may contribute to development of a clinical tool for methamphetamine addiction.
Title: Methamphetamine induces Shati/Nat8L expression in the mouse nucleus accumbens via CREB- and dopamine D1 receptor-dependent mechanism.
Results showed that both Shati+/- and Shati-/- mice demonstrated lower levels of NAA in the brain than Shati+/+ mice, suggesting that SHATI predominantly regulates NAA content in the brain
Title: Deletion of SHATI/NAT8L decreases the N-acetylaspartate content in the brain and induces behavioral deficits, which can be ameliorated by administering N-acetylaspartate.
Study shows that Shati/Nat8l in the neuron may induce axon outgrowth by ATP synthesis and not through mGluR3 signaling.
Title: Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons.
Dysregulated expression of Nat8L is associated with deficits in oxidative phosphorylation in 5xFAD model of Alzheimer disease.
Title: Transcriptional regulation of N-acetylaspartate metabolism in the 5xFAD model of Alzheimer's disease: evidence for neuron-glia communication during energetic crisis.
results indicate that Shati/Nat8l in the Nucleus Accumbens, but not in the dorsal striatum, plays a suppressive role in the behavioral responses to METHamphetamine by controlling the dopaminergic system via activation of group II mGlutamate receptors
Title: Overexpression of Shati/Nat8l, an N-acetyltransferase, in the nucleus accumbens attenuates the response to methamphetamine via activation of group II mGluRs in mice.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (4) 1 citation

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

Cyba (e-PCR), detects polymorphism
- UniSTS:548034

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables acyltransferase activity, transferring groups other than amino-acyl groups	IEA Inferred from Electronic Annotation more info
enables aspartate N-acetyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables aspartate N-acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables aspartate N-acetyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables aspartate N-acetyltransferase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in acetate metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in aspartate metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of dopamine uptake involved in synaptic transmission	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrial membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

Go to the top of the page Help

Preferred Names: N-acetylaspartate synthetase

Names: N-acetyltransferase 8-like protein; NAA synthase; NAA synthetase; aspartate N-acetyltransferase; protein Shati

NP_001001985.3

EC 2.3.1.17

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001001985.3 → NP_001001985.3 N-acetylaspartate synthetase

See identical proteins and their annotated locations for NP_001001985.3

Status: VALIDATED

Source sequence(s)

AC163329, AK147594

Consensus CDS

CCDS19210.1

UniProtKB/Swiss-Prot

Q3UGX3, Q3UH43, Q6DID6, Q8K065

UniProtKB/TrEMBL

A0A0R4J0R4

Related

ENSMUSP00000059313.9, ENSMUST00000056355.9

Conserved Domains (2) summary

COG0456
Location:146 → 270

RimI; Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis]

pfam00583
Location:187 → 262

Acetyltransf_1; Acetyltransferase (GNAT) family