Top2a DNA topoisomerase II alpha [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Top2aprovided by RGD
Official Full Name: DNA topoisomerase II alphaprovided by RGD
Primary source: RGD:62048
See related: EnsemblRapid:ENSRNOG00000053047 AllianceGenome:RGD:62048
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables several functions, including ATP binding activity; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; and chromatin binding activity. Involved in several processes, including DNA topological change; cerebellar cortex formation; and response to parathyroid hormone. Located in nucleolus. Part of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex. Orthologous to human TOP2A (DNA topoisomerase II alpha). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Thymus (RPKM 915.5), Spleen (RPKM 349.2) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q31

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (84441954..84473093, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (83945731..83976874, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (86901467..86930947, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

C-terminal domain of rat topo IIalpha is associated with efficient DNA catenation in vitro, based on comparison of wild-type rat topo IIalpha and its deletion mutants.
Title: DNA-binding activity of rat DNA topoisomerase II α C-terminal domain contributes to efficient DNA catenation in vitro.
The genotoxic and antigenotoxic effects of 8 chroman-2,4-diones are reported; the role of their TOPIIalpha inhibitory effect is described.
Title: Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach.
Data indicate that dexrazoxane may protect cardiomyocytes via its catalytic topoisomerase II (TOP2) inhibitory activity.
Title: Catalytic inhibitors of topoisomerase II differently modulate the toxicity of anthracyclines in cardiac and cancer cells.
Rac1-regulated mechanisms are required for DNA damage induction and subsequent activation of the DDR following treatment with topo II but not topo I poisons
Title: Rac1 protein signaling is required for DNA damage response stimulated by topoisomerase II poisons.
The Topoisomerase II alpha is predominantly localized in proliferative tissue and found in embryos up to post-natal day 1.Topoisomerase II beta in brain showed highest activity in cerebellar region and that too only neuronal cell fraction.
Title: Analysis of age dependent changes of Topoisomerase II alpha and beta in rat brain.
DNA binding activity exists in the head domain
Title: [Structural homology between molecular probes binding site of DNA polymerase beta and DNA topoisomerase II].

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC207566P1 (e-PCR)
- UniSTS:271954

PMC207566P6 (e-PCR)
- UniSTS:271959

RH128311 (e-PCR)
- UniSTS:211619

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	TAS Traceable Author Statement more info	PubMed
enables ATP-dependent activity, acting on DNA	ISO Inferred from Sequence Orthology more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA binding	TAS Traceable Author Statement more info	PubMed
enables DNA binding, bending	IEA Inferred from Electronic Annotation more info
enables DNA binding, bending	ISO Inferred from Sequence Orthology more info
enables DNA binding, bending	ISS Inferred from Sequence or Structural Similarity more info
enables DNA topoisomerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein heterodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein heterodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein kinase C binding	IEA Inferred from Electronic Annotation more info
enables protein kinase C binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA ligation	IEA Inferred from Electronic Annotation more info
involved_in DNA ligation	ISO Inferred from Sequence Orthology more info
involved_in DNA topological change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA topological change	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA topological change	ISO Inferred from Sequence Orthology more info
involved_in DNA topological change	ISO Inferred from Sequence Orthology more info
involved_in apoptotic chromosome condensation	IBA Inferred from Biological aspect of Ancestor more info
involved_in apoptotic chromosome condensation	IEA Inferred from Electronic Annotation more info
involved_in apoptotic chromosome condensation	ISO Inferred from Sequence Orthology more info
involved_in cerebellar Purkinje cell differentiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in cerebellar granule cell differentiation	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within chromosome condensation	ISO Inferred from Sequence Orthology more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chromosome segregation	ISO Inferred from Sequence Orthology more info
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
involved_in embryonic cleavage	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within embryonic cleavage	ISO Inferred from Sequence Orthology more info
involved_in female meiotic nuclear division	IEA Inferred from Electronic Annotation more info
involved_in female meiotic nuclear division	ISO Inferred from Sequence Orthology more info
involved_in hematopoietic progenitor cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within hematopoietic progenitor cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA duplex unwinding	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA duplex unwinding	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of single stranded viral RNA replication via double stranded DNA intermediate	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of single stranded viral RNA replication via double stranded DNA intermediate	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in regulation of circadian rhythm	IEA Inferred from Electronic Annotation more info
involved_in regulation of circadian rhythm	ISO Inferred from Sequence Orthology more info
involved_in regulation of circadian rhythm	ISS Inferred from Sequence or Structural Similarity more info
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to ionizing radiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to parathyroid hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in rhythmic process	IEA Inferred from Electronic Annotation more info
involved_in sister chromatid segregation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex	IDA Inferred from Direct Assay more info	PubMed
part_of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex	IEA Inferred from Electronic Annotation more info
part_of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex	ISO Inferred from Sequence Orthology more info
located_in centriole	IEA Inferred from Electronic Annotation more info
located_in centriole	ISO Inferred from Sequence Orthology more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	ISO Inferred from Sequence Orthology more info
located_in condensed chromosome	IEA Inferred from Electronic Annotation more info
located_in condensed chromosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in male germ cell nucleus	IEA Inferred from Electronic Annotation more info
located_in male germ cell nucleus	ISO Inferred from Sequence Orthology more info
located_in nuclear chromosome	IEA Inferred from Electronic Annotation more info
located_in nuclear chromosome	ISO Inferred from Sequence Orthology more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
NOT located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	IEA Inferred from Electronic Annotation more info
part_of ribonucleoprotein complex	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: DNA topoisomerase 2-alpha

Names: DNA topoisomerase II, alpha isozyme; topoisomerase (DNA) 2 alpha; topoisomerase (DNA) II alpha

NP_001380697.1

EC 5.6.2.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001393768.1 → NP_001380697.1 DNA topoisomerase 2-alpha

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/TrEMBL

A0A0G2JWP8, A0A8I6ADA9, A6HIW1

Related

ENSRNOP00000091216.2, ENSRNOT00000096403.2

Conserved Domains (2) summary

PTZ00108
Location:24 → 1443

PTZ00108; DNA topoisomerase 2-like protein; Provisional

pfam08070
Location:1433 → 1519

DTHCT; DTHCT (NUC029) region

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

84441954..84473093 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_022183.2: Suppressed sequence

Description

NM_022183.2: This RefSeq record was removed by NCBI staff. Contact info@ncbi.nlm.nih.gov for further information.