piwil1 piwi-like RNA-mediated gene silencing 1 [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: piwil1provided by ZNC
Official Full Name: piwi-like RNA-mediated gene silencing 1provided by ZNC
Primary source: ZFIN:ZDB-GENE-030813-2
See related: Ensembl:ENSDARG00000041699 AllianceGenome:ZFIN:ZDB-GENE-030813-2
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: ziwi
Summary: Enables piRNA binding activity. Involved in anatomical structure morphogenesis; germ cell development; and piRNA processing. Located in P granule. Is expressed in several structures, including blastodisc; central nervous system; eye; reproductive system; and unfertilized egg. Orthologous to several human genes including PIWIL1 (piwi like RNA-mediated gene silencing 1). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 8

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	8	NC_007119.7 (44224709..44240691, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	8	NC_007119.6 (44231290..44245207, complement)

Chromosome 8 - NC_007119.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Short communication: Differential expression of piwi1 and piwi2 genes in tissues of tambacu and zebrafish: A possible relationship with the indeterminate muscle growth.
Title: Short communication: Differential expression of piwi1 and piwi2 genes in tissues of tambacu and zebrafish: A possible relationship with the indeterminate muscle growth.
Tdrd1 acts as a molecular scaffold for Ziwi.
Title: Tdrd1 acts as a molecular scaffold for Piwi proteins and piRNA targets in zebrafish.
Promoter activity of a 4.8-kb sequence isolated from the 5' end of the zebrafish germ cell-specific ziwi gene is sufficient to drive gene expression in all germ cells with stage-specific differences in expression levels.
Title: The ziwi promoter drives germline-specific gene expression in zebrafish.
Ziwi and Zili bind piRNAs of opposite polarity
Title: Zili is required for germ cell differentiation and meiosis in zebrafish.
study demonstrated that zebrafish Piwi, Ziwi is expressed in gonad of both sexes and is a component of a structure called nuage; loss of Ziwi function results in a progressive loss of germ cells due to apoptosis during larval development
Title: A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish.

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General gene information

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Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	ISS Inferred from Sequence or Structural Similarity more info
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IEA Inferred from Electronic Annotation more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables piRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables piRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in anatomical structure morphogenesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in gamete generation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ cell development	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in piRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in piRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytoplasmic translation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated gene silencing	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulatory ncRNA-mediated gene silencing	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sperm DNA condensation	ISS Inferred from Sequence or Structural Similarity more info
involved_in spermatid development	ISS Inferred from Sequence or Structural Similarity more info
involved_in spermatogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in transposable element silencing by piRNA-mediated heterochromatin formation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
is_active_in P granule	IBA Inferred from Biological aspect of Ancestor more info
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: piwi-like protein 1

Names: piwi-like 1

NP_899181.1

EC 3.1.26.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_183338.2 → NP_899181.1 piwi-like protein 1

See identical proteins and their annotated locations for NP_899181.1

Status: VALIDATED

Source sequence(s)

CR450819

UniProtKB/Swiss-Prot

Q7ZWB5, Q8UVX0

Related

ENSDARP00000121147.1, ENSDART00000138019.3

Conserved Domains (4) summary

smart00949
Location:275 → 412

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:398 → 841

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...

pfam05831
Location:17 → 104

GAGE; GAGE protein

pfam16486
Location:104 → 203

ArgoN; N-terminal domain of argonaute