shg shotgun [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: shgprovided by FlyBase
Official Full Name: shotgunprovided by FlyBase
Primary source: FLYBASE:FBgn0003391
Locus tag: Dmel_CG3722
See related: AllianceGenome:FB:FBgn0003391
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: cad; Cad; CadE; cadh; Cadh; CADH; CG3722; com5; CT12481; D E-cad; D-cad; D-CAD2; DCad; DCAD; DCad2; DCAD2; DE; DE Cad; DE Cadh; de-cad; De-Cad; DE-cad; DE-Cad; DE-CAD; DE-Cad2; DE-CAD2; DE-cadh; DE-Cadh; dE-cadherin; DE-cadherin; DE-Cadherin; DEC; dEcad; DEcad; DECad; DECAD; DECadh; DE[cyto]; Dmel\CG3722; E-Ca; e-cad; E-cad; E-Cad; E-CAD; E-Cadh; E-cadherin; E-Cadherin; ecad; Ecad; ECad; ECAD; ECad2; ECadh; ECadherin; gp150; l(2)10469; l(2)k03401; l(2)k10220; N-cad; Shg; shg/DE-Cad; Shotgun
Summary: Enables calcium ion binding activity; myosin binding activity; and protein homodimerization activity. Involved in several processes, including animal organ development; gamete generation; and open tracheal system development. Located in several cellular components, including apical junction complex; recycling endosome; and spot adherens junction. Is integral component of plasma membrane. Part of catenin complex. Is expressed in several structures, including ectoderm; embryonic/larval circulatory system; epithelium; extended germ band embryo; and gut section. Used to study cancer and prostate cancer. [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 57B15-57B16; 2-92 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (21049654..21057197, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (16937159..16944702, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

E-cadherin variants associated with oral facial clefts trigger aberrant cell motility in a REG1A-dependent manner.
Title: E-cadherin variants associated with oral facial clefts trigger aberrant cell motility in a REG1A-dependent manner.
Src42A is required for E-cadherin dynamics at cell junctions during Drosophila axis elongation.
Title: Src42A is required for E-cadherin dynamics at cell junctions during Drosophila axis elongation.
Integrin beta1 orchestrates the abnormal cell-matrix attachment and invasive behaviour of E-cadherin dysfunctional cells.
Title: Integrin β1 orchestrates the abnormal cell-matrix attachment and invasive behaviour of E-cadherin dysfunctional cells.
DE-cadherin and Myosin II balance regulates furrow length for onset of polygon shape in syncytial Drosophila embryos.
Title: DE-cadherin and Myosin II balance regulates furrow length for onset of polygon shape in syncytial Drosophila embryos.
This work suggests distinct effects of tensile versus shear stresses on E-cadherin adhesion.
Title: Distinct contributions of tensile and shear stress on E-cadherin levels during morphogenesis.
Study showed that the highly evolutionary conserved Y654 beta-cat-D665-E-cad major interaction site of the adherens junctions complex acts as primary mechanosensor. The site activates the beta-cat pathway through its mechanical opening in vivo that enables phosphorylation by Src42A kinase.
Title: The major β-catenin/E-cadherin junctional binding site is a primary molecular mechano-transductor of differentiation in vivo.
In disc-proper epithelial cells, DE-cadherin localizes to the zonula adherens, and in puncta at the basal, and lateral sides. Fra overexpression of affects DE-Cadherin distribution and apico-basal cell shape so that there is loss of DE-Cadherin in the basal and lateral regions.
Title: Frazzled can act through distinct molecular pathways in epithelial cells to regulate motility, apical constriction, and localisation of E-Cadherin.
several phospho-mutations dramatically reduced the biosynthetic turnover rate of DE-Cad during apical-basal polarization.
Title: Phosphorylation potential of Drosophila E-Cadherin intracellular domain is essential for development and adherens junction biosynthetic dynamics regulation.
Transmission of cytokinesis forces via E-cadherin dilution and actomyosin flows
Title: Transmission of cytokinesis forces via E-cadherin dilution and actomyosin flows.
alpha-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability.
Title: α-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction.

Submit: New GeneRIF Correction See all GeneRIFs (58)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0814 (e-PCR)
- UniSTS:52832

Dm0373 (e-PCR)
- UniSTS:12167

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables beta-catenin binding	IPI Inferred from Physical Interaction more info	PubMed
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables myosin binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in apical protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in brain development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in branch fusion, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules	IDA Inferred from Direct Assay more info	PubMed
involved_in cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell competition in a multicellular organism	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell elongation involved in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell projection assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell-cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell-cell adhesion mediated by cadherin	IDA Inferred from Direct Assay more info	PubMed
involved_in compound eye morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to fungus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in determination of digestive tract left/right asymmetry	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female germ-line stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gastrulation involving germ band extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gonad development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gonad morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in gonad morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gonadal mesoderm development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in head involution	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hemocyte migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in homophilic cell adhesion via plasma membrane adhesion molecules	IDA Inferred from Direct Assay more info	PubMed
involved_in homophilic cell adhesion via plasma membrane adhesion molecules	IEA Inferred from Electronic Annotation more info
involved_in insect visual primordium development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of epithelial integrity, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male germline stem cell symmetric division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in morphogenesis of a polarized epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ommatidial rotation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte localization involved in germarium-derived egg chamber formation	TAS Traceable Author Statement more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in open tracheal system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in outflow tract morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of receptor signaling pathway via JAK-STAT	IGI Inferred from Genetic Interaction more info	PubMed
involved_in salivary gland morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in segment specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in somatic stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in wound healing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in zonula adherens assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in adherens junction	IDA Inferred from Direct Assay more info	PubMed
located_in apical junction complex	IDA Inferred from Direct Assay more info	PubMed
part_of catenin complex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in filopodium	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in recycling endosome	IDA Inferred from Direct Assay more info	PubMed
located_in spot adherens junction	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: shotgun

Names: (DE)-cadherin; CG3722-PA; Cadherin-E; D-Ecadherin; D-cadherin; DCAD2; DE cadherin; DE-cad; DE-cadherin/Shotgun; DEcad; DEcadherin; De-cadherin; ECadherin; Shotgun/E-cadherin; cadherin; compaction defects 5; drosophila E-cadherin; drosophila epithelial cadherin; drosophila melanogaster epithelial (DE)-cadherin; e-cadherin; epithelial cadherin; eta-catenin; shg-PA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

21049654..21057197 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057374.3 → NP_476722.1 shotgun [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476722.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q24298, Q9W2N1

Related

FBpp0071475

Conserved Domains (4) summary

cd11304
Location:93 → 191

Cadherin_repeat; Cadherin tandem repeat domain

smart00112
Location:332 → 410

CA; Cadherin repeats

cd00110
Location:1127 → 1294

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam01049
Location:1358 → 1489

Cadherin_C; Cadherin cytoplasmic region