U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Rffl ring finger and FYVE like domain containing protein [ Mus musculus (house mouse) ]

Gene ID: 67338, updated on 17-Aug-2024

Summary

Go to the top of the page Help
Official Symbol
Rfflprovided by MGI
Official Full Name
ring finger and FYVE like domain containing proteinprovided by MGI
Primary source
MGI:MGI:1914588
See related
Ensembl:ENSMUSG00000020696 AllianceGenome:MGI:1914588
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Carp2; 1700051E09Rik; 4930516L10Rik
Summary
Predicted to enable enzyme binding activity; p53 binding activity; and ubiquitin protein ligase activity. Involved in regulation of fibroblast migration. Acts upstream of or within intracellular protein transport. Located in cytoplasmic vesicle. Is expressed in bladder; retina inner nuclear layer; retina outer nuclear layer; and ureter. Orthologous to human RFFL (ring finger and FYVE like domain containing E3 ubiquitin protein ligase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in testis adult (RPKM 17.6), placenta adult (RPKM 7.7) and 28 other tissues See more
Orthologs
human all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
11 C; 11 50.25 cM
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (82694645..82762065, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (82803819..82871236, complement)

Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E7027 Neighboring gene zinc finger protein 830 Neighboring gene predicted gene 11423 Neighboring gene ligase III, DNA, ATP-dependent Neighboring gene STARR-seq mESC enhancer starr_30179 Neighboring gene RAD51 paralog D Neighboring gene STARR-positive B cell enhancer ABC_E8433 Neighboring gene fibronectin type III domain containing 8

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. CARP2 deficiency does not alter induction of NF-kappaB by TNFalpha. Ahmed AU, et al. Curr Biol, 2009 Jan 13. PMID 19138581
  2. Different Raf protein kinases mediate different signaling pathways to stimulate E3 ligase RFFL gene expression in cell migration regulation. Gan X, et al. J Biol Chem, 2013 Nov 22. PMID 24114843, Free PMC Article
  3. CARP-2 is an endosome-associated ubiquitin ligase for RIP and regulates TNF-induced NF-kappaB activation. Liao W, et al. Curr Biol, 2008 May 6. PMID 18450452, Free PMC Article
  4. Transcript map of the Ovum mutant (Om) locus: isolation by exon trapping of new candidate genes for the DDK syndrome. Le Bras S, et al. Gene, 2002 Aug 21. PMID 12383505
  5. PRR5L degradation promotes mTORC2-mediated PKC-δ phosphorylation and cell migration downstream of Gα12. Gan X, et al. Nat Cell Biol, 2012 May 20. PMID 22609986, Free PMC Article

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. EGF, which signals through CRAF, and an activated BRAF mutant also activate PKC and stimulate cell migration through up-regulating RFFL expression.
    Title: Different Raf protein kinases mediate different signaling pathways to stimulate E3 ligase RFFL gene expression in cell migration regulation.
  2. CARP2 [Rififylin] deficiency does not alter induction of NF-kappaB by TNFalpha.
    Title: CARP2 deficiency does not alter induction of NF-kappaB by TNFalpha.
  3. Data show that the the amino-terminal domain of rififylin is critical for its recruitment to recycling endocytic membranes and for the inhibition of recycling.
    Title: Over-expression of Rififylin, a new RING finger and FYVE-like domain-containing protein, inhibits recycling from the endocytic recycling compartment.
Submit: New GeneRIF Correction

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (3) 
  • Gene trapped (1) 
  • Targeted (1)  1 citation

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • MGC144763

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables p53 binding ISO
Inferred from Sequence Orthology
more info
  
enables protease binding ISO
Inferred from Sequence Orthology
more info
  
enables protein kinase binding ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin protein ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within apoptotic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within intracellular protein transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of signal transduction by p53 class mediator ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of tumor necrosis factor-mediated signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in protein K48-linked ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein K48-linked ubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of fibroblast migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ubiquitin-dependent protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasmic vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endosome IEA
Inferred from Electronic Annotation
more info
  
located_in endosome membrane ISO
Inferred from Sequence Orthology
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

Go to the top of the page Help
Preferred Names
E3 ubiquitin-protein ligase rififylin
Names
RING finger and FYVE-like domain-containing protein 1
RING-type E3 ubiquitin transferase rififylin
fring
rififylin
NP_001007466.1
NP_001158041.1
NP_001158042.1
NP_001158043.1
NP_080373.1
XP_006534050.1
XP_006534051.1
XP_006534052.1
XP_006534053.2
XP_017170204.1
XP_030102107.1
XP_036012800.1
XP_036012801.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001007465.3 → NP_001007466.1  E3 ubiquitin-protein ligase rififylin isoform 1

    See identical proteins and their annotated locations for NP_001007466.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes isoform 1.
    Source sequence(s)
    AI850798, AK150213, AK169393, AL646089, CJ097615
    Consensus CDS
    CCDS25148.1
    UniProtKB/TrEMBL
    Q3UD78
    Related
    ENSMUSP00000103808.4, ENSMUST00000108173.10
    Conserved Domains (2) summary
    cd15770
    Location:44 → 92
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:315 → 353
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  2. NM_001164569.1 → NP_001158041.1  E3 ubiquitin-protein ligase rififylin isoform 3

    See identical proteins and their annotated locations for NP_001158041.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) includes an additional segment in the 5' UTR and 5' coding region, and uses an alternate translational start codon, compared to variant 1. The resulting isoform (3) is longer at the N-terminus, compared to isoform 1.
    Source sequence(s)
    AI850798, AK150541, AL645594, AL646089
    Consensus CDS
    CCDS48868.1
    UniProtKB/TrEMBL
    Q3UCG9
    Related
    ENSMUSP00000071150.8, ENSMUST00000071152.14
    Conserved Domains (2) summary
    cd15770
    Location:79 → 127
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:350 → 388
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  3. NM_001164570.1 → NP_001158042.1  E3 ubiquitin-protein ligase rififylin isoform 4

    See identical proteins and their annotated locations for NP_001158042.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region, and uses an alternate translational start codon, compared to variant 1. The resulting isoform (4) is longer at the N-terminus, compared to isoform 1.
    Source sequence(s)
    AI850798, AL645594, AL646089
    Consensus CDS
    CCDS48867.1
    UniProtKB/Swiss-Prot
    Q5SVC2, Q5SVC4, Q6ZQM0, Q9D543, Q9D9B1
    Related
    ENSMUSP00000091510.6, ENSMUST00000093975.12
    Conserved Domains (2) summary
    cd15770
    Location:58 → 106
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:329 → 367
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  4. NM_001164571.1 → NP_001158043.1  E3 ubiquitin-protein ligase rififylin isoform 5

    See identical proteins and their annotated locations for NP_001158043.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (5) is shorter than isoform 1.
    Source sequence(s)
    AI850798, AK169393, AL646089, CJ097615
    UniProtKB/TrEMBL
    Q3TEV8
    Conserved Domains (2) summary
    pfam13920
    Location:285 → 329
    zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)
    cl22851
    Location:44 → 93
    PHD_SF; PHD finger superfamily

  5. NM_026097.3 → NP_080373.1  E3 ubiquitin-protein ligase rififylin isoform 2

    See identical proteins and their annotated locations for NP_080373.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AI850798, AK015806, AK028095, AK169393
    Consensus CDS
    CCDS25149.1
    UniProtKB/TrEMBL
    Q148A8
    Related
    ENSMUSP00000021036.7, ENSMUST00000021036.13
    Conserved Domains (2) summary
    cd15770
    Location:44 → 92
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:287 → 325
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000077.7 Reference GRCm39 C57BL/6J

    Range
    82694645..82762065 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030246247.2 → XP_030102107.1  E3 ubiquitin-protein ligase rififylin isoform X6

    UniProtKB/TrEMBL
    Q148A8
    Conserved Domains (2) summary
    cd15770
    Location:44 → 92
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:287 → 325
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  2. XM_036156907.1 → XP_036012800.1  E3 ubiquitin-protein ligase rififylin isoform X3

    UniProtKB/Swiss-Prot
    Q5SVC2, Q5SVC4, Q6ZQM0, Q9D543, Q9D9B1
    Conserved Domains (2) summary
    cd15770
    Location:58 → 106
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:329 → 367
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  3. XM_017314715.3 → XP_017170204.1  E3 ubiquitin-protein ligase rififylin isoform X2

    UniProtKB/TrEMBL
    Q3UCG9
    Conserved Domains (2) summary
    cd15770
    Location:79 → 127
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:350 → 388
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  4. XM_006533990.4 → XP_006534053.2  E3 ubiquitin-protein ligase rififylin isoform X1

    Conserved Domains (2) summary
    cd15770
    Location:113 → 161
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:384 → 422
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  5. XM_036156908.1 → XP_036012801.1  E3 ubiquitin-protein ligase rififylin isoform X6

    UniProtKB/TrEMBL
    Q148A8
    Conserved Domains (2) summary
    cd15770
    Location:44 → 92
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:287 → 325
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  6. XM_006533989.4 → XP_006534052.1  E3 ubiquitin-protein ligase rififylin isoform X5

    See identical proteins and their annotated locations for XP_006534052.1

    UniProtKB/TrEMBL
    Q3UD78
    Conserved Domains (2) summary
    cd15770
    Location:44 → 92
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:315 → 353
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  7. XM_006533988.3 → XP_006534051.1  E3 ubiquitin-protein ligase rififylin isoform X5

    See identical proteins and their annotated locations for XP_006534051.1

    UniProtKB/TrEMBL
    Q3UD78
    Related
    ENSMUSP00000074108.5, ENSMUST00000074515.11
    Conserved Domains (2) summary
    cd15770
    Location:44 → 92
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:315 → 353
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins

  8. XM_006533987.4 → XP_006534050.1  E3 ubiquitin-protein ligase rififylin isoform X4

    Conserved Domains (2) summary
    cd15770
    Location:79 → 127
    FYVE_CARP2; FYVE-like domain found in caspase regulator CARP2 and similar proteins
    cd16500
    Location:322 → 360
    RING-HC_CARP; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein CARP-1, CARP-2 and similar proteins
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q6ZQM0.1 GenPept UniProtKB/Swiss-Prot:Q6ZQM0

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous