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Hyal5 hyaluronoglucosaminidase 5 [ Mus musculus (house mouse) ]

Gene ID: 74468, updated on 17-Aug-2024

Summary

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Official Symbol
Hyal5provided by MGI
Official Full Name
hyaluronoglucosaminidase 5provided by MGI
Primary source
MGI:MGI:1921718
See related
Ensembl:ENSMUSG00000029678 AllianceGenome:MGI:1921718
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
4933439A12Rik
Summary
Enables hyalurononglucosaminidase activity. Acts upstream of or within penetration of zona pellucida. Located in acrosomal vesicle and external side of plasma membrane. Orthologous to human SPAM1 (sperm adhesion molecule 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Restricted expression toward testis adult (RPKM 24.7) See more
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Genomic context

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See Hyal5 in Genome Data Viewer
Location:
6 A3.1; 6 11.28 cM
Exon count:
5
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (24857982..24891963)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (24857937..24891959)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_15361 Neighboring gene sperm adhesion molecule 1 Neighboring gene sperm adhesion molecule 1 pseudogene Neighboring gene predicted gene, 22267 Neighboring gene STARR-seq mESC enhancer starr_15363 Neighboring gene STARR-seq mESC enhancer starr_15364 Neighboring gene STARR-seq mESC enhancer starr_15365 Neighboring gene transmembrane protein 229A

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect. Park S, et al. FASEB J, 2019 Dec. PMID 31670981
  2. Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization? Kang W, et al. J Reprod Dev, 2010 Feb. PMID 19926939
  3. Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization. Kimura M, et al. Biol Reprod, 2009 Nov. PMID 19605784
  4. Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but not HYALP1 degrade hyaluronan. Reitinger S, et al. Biochem J, 2007 Jan 1. PMID 16925524, Free PMC Article
  5. Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass. Kim E, et al. Proc Natl Acad Sci U S A, 2005 Dec 13. PMID 16330764, Free PMC Article

See all (17) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A comparative analysis between WT and Spam1/Hyal5 dKO epididymal sperm revealed that the absence of these 2 sperm Hyals resulted in a marked accumulation of sperm on the outside of the cumulus-oocyte complex.
    Title: SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect.
  2. The data from this paper suggests that the hyaluronan-degrading activity of the sperm hyaluronidase HYAL5 may not be required for fertilization, at least in the mouse.
    Title: Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization?
  3. The HYAL5-deficient female mice were fully fertile and yielded normal litter sizes. HYAL5-deficient epididymal sperm is functionally normal. Thus, HYAL5 may be dispensable for fertilization.
    Title: Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization.
  4. Mouse SPAM1 and HYAL5 but not HYALP1 proteins exhibit hyaluronidase activity at neutral pH.
    Title: Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but not HYALP1 degrade hyaluronan.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables hyalurononglucosaminidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables hyalurononglucosaminidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in fusion of sperm to egg plasma membrane involved in single fertilization IEA
Inferred from Electronic Annotation
more info
  
involved_in hyaluronan catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within penetration of zona pellucida IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in acrosomal vesicle IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in acrosomal vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasmic vesicle IEA
Inferred from Electronic Annotation
more info
  
located_in external side of plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in side of membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
hyaluronidase-5
Names
hyal-5
hyaluronidase Hyal-5
hyaluronidase PH-20
NP_001404776.1
NP_001404777.1
NP_001404778.1
NP_083233.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001417847.1 → NP_001404776.1  hyaluronidase-5 isoform a precursor

    Status: VALIDATED

    Source sequence(s)
    AC127559
    UniProtKB/Swiss-Prot
    Q812F3
    UniProtKB/TrEMBL
    Q9CUA5
    Related
    ENSMUSP00000031689.6, ENSMUST00000031689.6

  2. NM_001417848.1 → NP_001404777.1  hyaluronidase-5 isoform b

    Status: VALIDATED

    Source sequence(s)
    AC127559

  3. NM_001417849.1 → NP_001404778.1  hyaluronidase-5 isoform c

    Status: VALIDATED

    Source sequence(s)
    AC127559

  4. NM_028957.3 → NP_083233.1  hyaluronidase-5 isoform a precursor

    See identical proteins and their annotated locations for NP_083233.1

    Status: VALIDATED

    Source sequence(s)
    AC127559
    Consensus CDS
    CCDS19948.1
    UniProtKB/Swiss-Prot
    Q812F3
    UniProtKB/TrEMBL
    Q9CUA5
    Related
    ENSMUSP00000144011.2, ENSMUST00000200968.4
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    24857982..24891963
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q812F3.1 GenPept UniProtKB/Swiss-Prot:Q812F3

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