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BUD6 Bud6p [ Saccharomyces cerevisiae S288C ]

Gene ID: 851029, updated on 18-Sep-2024

Summary

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Official Symbol
BUD6
Official Full Name
Bud6p
Primary source
SGD:S000004311
Locus tag
YLR319C
See related
AllianceGenome:SGD:S000004311; FungiDB:YLR319C; VEuPathDB:YLR319C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
AIP3
Summary
Enables actin binding activity; cytoskeletal regulatory protein binding activity; and enzyme activator activity. Involved in several processes, including bipolar cellular bud site selection; cytoskeleton organization; and positive regulation of cytoskeleton organization. Located in several cellular components, including cellular bud tip; mating projection tip; and spindle pole body. Part of incipient cellular bud site and polarisome. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See BUD6 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (769318..771684, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Nkp2p Neighboring gene Tad3p Neighboring gene telomerase reverse transcriptase Neighboring gene Mms22p Neighboring gene Sfh1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth. Luedeke C, et al. J Cell Biol, 2005 Jun 20. PMID 15967812, Free PMC Article
  2. Kar9p-independent microtubule capture at Bud6p cortical sites primes spindle polarity before bud emergence in Saccharomyces cerevisiae. Segal M, et al. Mol Biol Cell, 2002 Dec. PMID 12475941, Free PMC Article
  3. Fission yeast Aip3p (spAip3p) is required for an alternative actin-directed polarity program. Jin H, et al. Mol Biol Cell, 2001 May. PMID 11359921, Free PMC Article
  4. Severing of F-actin by yeast cofilin is pH-independent. Pavlov D, et al. Cell Motil Cytoskeleton, 2006 Sep. PMID 16847879, Free PMC Article
  5. Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Amberg DC, et al. Mol Biol Cell, 1997 Apr. PMID 9247651, Free PMC Article

See all (111) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A teamwork promotion of formin-mediated actin nucleation by Bud6 and Aip5 in Saccharomyces cerevisiae.
    Title: A teamwork promotion of formin-mediated actin nucleation by Bud6 and Aip5 in Saccharomyces cerevisiae.
  2. Structure of a Bud6/Actin complex reveals a novel wh2-like actin monomer recruitment motif.
    Title: Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif.
  3. Yor304c-a/Bil1 colocalizes with Bud6 and functions in the Bnr1 pathway for actin assembly
    Title: Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation.
  4. model in which a Bni1/Bud6 complex with a 2:4 subunit stoichiometry assembles a nucleation seed with Bud6 coordinating up to four actin subunits
    Title: Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6.
  5. Bud6p and Kip3p play antagonistic roles in controlling the length of microtubules contacting the bud.
    Title: Mechanism for astral microtubule capture by cortical Bud6p priming spindle polarity in S. cerevisiae.
  6. Bud6 promotes actin filament nucleation by recruiting actin monomers to Bni1
    Title: Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor.
  7. BNI1 and BNR1 contribute to Bud6p-dependent microtubule-cortex interactions by controlling Bud6p localisation.
    Title: Dissecting the involvement of formins in Bud6p-mediated cortical capture of microtubules in S. cerevisiae.
  8. Bni1 and Bnr1 are regulated by Bud6
    Title: Differential activities and regulation of Saccharomyces cerevisiae formin proteins Bni1 and Bnr1 by Bud6.
  9. Bud6p, through its localization at both spindle pole bodies and at the mother-bud neck, provides a regulatory mechanism to prevent excessive oscillations of preanaphase nuclei, thus reducing the likelihood of mitotic delays and nuclear missegregation.
    Title: Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.
  10. Bud6p has a role in MT capture at the cell cortex, independent of Kar9p-mediated MT delivery along actin cables
    Title: Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables actin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables cytoskeletal regulatory protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables cytoskeletal regulatory protein binding IEA
Inferred from Electronic Annotation
more info
  
enables cytoskeletal regulatory protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables enzyme activator activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in actin filament bundle assembly IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in actin filament bundle assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in astral microtubule organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in bipolar cellular bud site selection IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in budding cell apical bud growth IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in budding cell apical bud growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular hyperosmotic response IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in cellular hyperosmotic response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in establishment of cell polarity IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in establishment of cell polarity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of actin nucleation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of actin nucleation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of formin-nucleated actin cable assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of formin-nucleated actin cable assembly IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in pseudohyphal growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of actin cytoskeleton organization IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of actin cytoskeleton organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of protein localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cell tip IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cellular bud neck IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cellular bud tip IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in incipient cellular bud site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mating projection tip HDA PubMed 
located_in mating projection tip IDA
Inferred from Direct Assay
more info
 PubMed 
part_of polarisome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in prospore membrane HDA PubMed 
located_in secretory vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle pole body IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
Bud6p
NP_013423.1
  • Actin- and formin-interacting protein; nucleation-promoting factor (NPF) for Bni1p and Bnr1p during actin cable assembly and organization; enhances Bnr1p-mediated actin nucleation, alleviating Hof1p inhibition through competitive binding; interacts with Bni1p via a C-terminal triple helical coiled-coil domain; role in polarized cell growth; isolated as a bipolar budding mutant; localizes to the presumptive bud site, small bud tips, the bud neck and secretory vesicles; potential Cdc28p substrate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    769318..771684 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182208.1 → NP_013423.1  TPA: Bud6p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013423.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYW2, P41697
    UniProtKB/TrEMBL
    A7A1K3, C7GUB4, G2WJE1, N1P006
    Conserved Domains (1) summary
    smart00806
    Location:365 → 776
    AIP3; Actin interacting protein 3
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P41697.1

Gene LinkOut

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