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DIF1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851157, updated on 11-Sep-2024

Summary

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Official Symbol
DIF1
Primary source
SGD:S000004429
Locus tag
YLR437C
See related
AllianceGenome:SGD:S000004429; FungiDB:YLR437C; VEuPathDB:YLR437C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
SDH1
Summary
Predicted to enable ribonucleoside-diphosphate reductase inhibitor activity. Involved in protein import into nucleus. Is active in cytoplasm. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (1011622..1012023, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Tsr2p Neighboring gene Ecm30p Neighboring gene ornithine-oxo-acid transaminase Neighboring gene U4/U6-U5 snRNP complex subunit LSM3

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Phenotypes associated with Saccharomyces cerevisiae Hug1 protein, a putative negative regulator of dNTP Levels, reveal similarities and differences with sequence-related Dif1. Kim E, et al. J Microbiol, 2011 Feb. PMID 21369983
  2. Yeast Dun1 Kinase Regulates Ribonucleotide Reductase Small Subunit Localization in Response to Iron Deficiency. Sanvisens N, et al. J Biol Chem, 2016 Apr 29. PMID 26970775, Free PMC Article
  3. Hug1 is an intrinsically disordered protein that inhibits ribonucleotide reductase activity by directly binding Rnr2 subunit. Meurisse J, et al. Nucleic Acids Res, 2014 Dec 1. PMID 25378334, Free PMC Article
  4. Regulation of ribonucleotide reductase by Spd1 involves multiple mechanisms. Nestoras K, et al. Genes Dev, 2010 Jun 1. PMID 20516199, Free PMC Article
  5. Dif1 controls subcellular localization of ribonucleotide reductase by mediating nuclear import of the R2 subunit. Wu X, et al. Mol Cell Biol, 2008 Dec. PMID 18838542, Free PMC Article

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. In contrast to a deletion of structurally related Difl, Rnr2-Rnr4 subcellular localization is not grossly altered in a Hugl deletion mutant.
    Title: Phenotypes associated with Saccharomyces cerevisiae Hug1 protein, a putative negative regulator of dNTP Levels, reveal similarities and differences with sequence-related Dif1.
  2. Dif1 is required for nuclear import of the R2 subunit and plays an essential role in regulating the dynamic RNR subcellular localization.
    Title: Dif1 controls subcellular localization of ribonucleotide reductase by mediating nuclear import of the R2 subunit.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
enables ribonucleoside-diphosphate reductase inhibitor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in protein import into nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein localization IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Dif1p
NP_013541.3
  • Protein that regulates nuclear import of Rnr2p and Rnr4p; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half shows similarity to S. pombe Spd1 protein; DIF1 has a paralog, SML1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    1011622..1012023 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182325.3 → NP_013541.3  TPA: Dif1p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013541.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    B3RHU5, C8ZE43, D6VZ71, O13577
    UniProtKB/TrEMBL
    G2WJP7, N1P1Y3
    Conserved Domains (1) summary
    pfam08591
    Location:38 → 66
    RNR_inhib; Ribonucleotide reductase inhibitor
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O13577.1

Gene LinkOut

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