CKS1 cyclin-dependent protein kinase regulatory subunit CKS1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CKS1
Gene description: cyclin-dependent protein kinase regulatory subunit CKS1
Primary source: FungiDB:YBR135W
Locus tag: YBR135W
See related: SGD:S000000339; AllianceGenome:SGD:S000000339; VEuPathDB:YBR135W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables histone binding activity; protein kinase activator activity; and ubiquitin binding activity. Involved in positive regulation of transcription by RNA polymerase II; regulation of mitotic cell cycle; and regulation of nucleosome density. Located in cytoplasm and nucleus. Part of cyclin-dependent protein kinase holoenzyme complex. Used to study breast cancer; liver cancer; and lung cancer. Orthologous to several human genes including CKS2 (CDC28 protein kinase regulatory subunit 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See CKS1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (504854..505306)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mutation of the polo-like kinase Cdc5, which functions in both the mitotic entry and mitotic exit pathways, is lethal in combination with overexpressed CKS1
Title: A Synthetic Dosage Lethal Genetic Interaction Between CKS1B and PLK1 Is Conserved in Yeast and Human Cancer Cells.
Cks1 mediates recruitment of the 19S proteasome to the Paf1 complex, thereby promoting transcriptional elongation.
Title: Cks1 enhances transcription efficiency at the GAL1 locus by linking the Paf1 complex to the 19S proteasome.
conservation of Cks hinge-region prolines reflects their functions in forming a Cdk binding interface and that ability of these prolines to control partitioning between monomer and dimer is a consequence of the beta-turn networks within the hinge.
Title: The structure of a monomeric mutant Cks protein reveals multiple functions for a conserved hinge-region proline.
These data suggest a model where binding of a complex consisting of Cks1, Cdk1, and the 19S proteasome to histone H4 leads to removal of nucleosomes via a nonproteolytic activity of the proteasome.
Title: Cks1, Cdk1, and the 19S proteasome collaborate to regulate gene induction-dependent nucleosome eviction in yeast.
function in transcription for the cell cycle regulatory cyclin-dependent kinase Cdc28 (Cdk1) and its interacting protein, Cks1
Title: A kinase-independent function of Cks1 and Cdk1 in regulation of transcription.
the first report of a direct physical interaction between ubiquitin and Cks1p, inferring a role for ubiquitin binding in the transcriptional function of Cks1p
Title: The alpha helix of ubiquitin interacts with yeast cyclin-dependent kinase subunit CKS1.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables cyclin-dependent protein serine/threonine kinase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables cyclin-dependent protein serine/threonine kinase regulator activity	IEA Inferred from Electronic Annotation more info
enables histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables histone binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cyclin-dependent protein kinase holoenzyme complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cyclin-dependent protein kinase holoenzyme complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed

General protein information

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Preferred Names: cyclin-dependent protein kinase regulatory subunit CKS1

NP_009693.3

Cyclin-dependent protein kinase regulatory subunit and adaptor; interacts with Cdc28p (aka Cdk1p); required for G1/S and G2/M phase transitions and budding; mediates phosphorylation and degradation of Sic1p; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters; human homologs CKS1B and CKS2 can each complement yeast cks1 null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

504854..505306

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178483.3 → NP_009693.3 TPA: cyclin-dependent protein kinase regulatory subunit CKS1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009693.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQD1, P20486

UniProtKB/TrEMBL

A0A6A5Q038, A6ZL78, B3LN22, C7GY17, D3UEM9, G2W9D8, N1PAT5

Conserved Domains (1) summary

pfam01111
Location:30 → 104

CKS; Cyclin-dependent kinase regulatory subunit