RGD1 GTPase-activating protein RGD1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RGD1
Official Full Name: GTPase-activating protein RGD1
Primary source: SGD:S000000464
Locus tag: YBR260C
See related: AllianceGenome:SGD:S000000464; FungiDB:YBR260C; VEuPathDB:YBR260C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables GTPase activator activity and phosphatidylinositol phosphate binding activity. Involved in response to acidic pH and response to osmotic stress. Acts upstream of or within actin cytoskeleton reorganization. Located in actin cortical patch and cellular bud. Orthologous to several human genes including ARHGAP15 (Rho GTPase activating protein 15) and ARHGAP27 (Rho GTPase activating protein 27). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RGD1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (732639..734639, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Role of RhoGAP Rgd1 in Pkc1 signaling-related actin repolarization under heat shock stress in Saccharomyces cerevisiae.
Title: Role of RhoGAP Rgd1 in Pkc1 signaling-related actin repolarization under heat shock stress in Saccharomyces cerevisiae.
demonstrate that Rgd1p is phosphorylated by the Aurora B like kinase Ipl1 and observe that loss of Ipl1 function leads to a new Rgd1p distribution in a small part of the cell population
Title: The Saccharomyces cerevisiae RhoGAP Rgd1 is phosphorylated by the Aurora B like kinase Ipl1.
Rgd1p colocalizes with a subset of the post-golgi marker Sec2p and is transported to the bud tip.
Title: Secretory pathway-dependent localization of the Saccharomyces cerevisiae Rho GTPase-activating protein Rgd1p at growth sites.
The authors propose that Tos2p, a protein involved in polarized growth and most probably associated with the plasma membrane, modulates the action of Rgd1p and Rho3p in Saccharomyces cerevisiae.
Title: Evidence for functional links between the Rgd1-Rho3 RhoGAP-GTPase module and Tos2, a protein involved in polarized growth in Saccharomyces cerevisiae.
the first evidences for a specific interaction between a RhoGAP domain (the RhoGAP domain of Rgd1p) and phosphoinositides were provided.
Title: Evidence for specific interaction between the RhoGAP domain from the yeast Rgd1 protein and phosphoinositides.
Phosphoinositides both regulate the recruitment and trafficking of Rgd1p to membranes via the F-BAR domain and specifically stimulate GTPase-activating protein activity
Title: Phosphoinositides affect both the cellular distribution and activity of the F-BAR-containing RhoGAP Rgd1p in yeast.
The rgd1Delta strain is sensitive to low pH, and Rgd1p activates the PKC pathway in an acidic environment
Title: The Rgd1p Rho GTPase-activating protein and the Mid2p cell wall sensor are required at low pH for protein kinase C pathway activation and cell survival in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	IGI Inferred from Genetic Interaction more info	PubMed
enables phosphatidylinositol-3,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3,5-bisphosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol-3-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3-phosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol-5-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-5-phosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of cell polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to acidic pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to osmotic stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in septin ring organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in small GTPase-mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cell division site	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cellular bud	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mating projection tip	HDA more info	PubMed
is_active_in mating projection tip	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in prospore membrane	HDA more info	PubMed

General protein information

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Preferred Names: GTPase-activating protein RGD1

NP_009819.1

GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; involved in repolarization of actin cytoskeleton during adaptation to heat shock stress; phosphorylated by Pkc1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

732639..734639 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178608.1 → NP_009819.1 TPA: GTPase-activating protein RGD1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009819.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQQ7, P38339

UniProtKB/TrEMBL

A6ZLK0, B3LMQ1, C7GMX0, D3UF08, G2W9R5, N1PAW2

Conserved Domains (2) summary

cd07652
Location:39 → 268

F-BAR_Rgd1; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Saccharomyces cerevisiae Rho GTPase activating protein Rgd1 and similar proteins

cd04398
Location:470 → 663

RhoGAP_fRGD1; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal RGD1-like proteins. Yeast Rgd1 is a GAP protein for Rho3 and Rho4 and plays a role in low-pH response. Small GTPases cluster into distinct families, and all act as ...