ERV1 flavin-linked sulfhydryl oxidase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ERV1
Official Full Name: flavin-linked sulfhydryl oxidase
Primary source: SGD:S000003261
Locus tag: YGR029W
See related: AllianceGenome:SGD:S000003261; FungiDB:YGR029W; VEuPathDB:YGR029W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables flavin-linked sulfhydryl oxidase activity. Involved in cellular iron ion homeostasis; cellular response to oxidative stress; and protein import into mitochondrial intermembrane space. Located in mitochondrial intermembrane space. Used to study autosomal dominant polycystic kidney disease and autosomal recessive centronuclear myopathy. Human ortholog(s) of this gene implicated in kidney failure and renal fibrosis. Orthologous to human GFER (growth factor, augmenter of liver regeneration). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ERV1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 2

Sequence:: Chromosome: VII; NC_001139.9 (543553..544205)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Redox characterisation of Erv1, a key component for protein import and folding in yeast mitochondria.
Title: Redox characterisation of Erv1, a key component for protein import and folding in yeast mitochondria.
Effects of Liposome and Cardiolipin on Folding and Function of Mitochondrial Erv1.
Title: Effects of Liposome and Cardiolipin on Folding and Function of Mitochondrial Erv1.
Kinetic characterisation of Erv1, a key component for protein import and folding in yeast mitochondria.
Title: Kinetic characterisation of Erv1, a key component for protein import and folding in yeast mitochondria.
Chimeras between the N-terminal arm of ScErv1 and a variety of truncated LtErv constructs were able to rescue yeast cells that lack ScErv1.
Title: A single-cysteine mutant and chimeras of essential Leishmania Erv can complement the loss of Erv1 but not of Mia40 in yeast.
Authors show that the fumarate reductase Osm1, which facilitates electron transfer from fumarate to succinate, fills this gap as a new electron acceptor. In addition to microsomes, Osm1 localizes to the mitochondrial intermembrane space and assembles with Erv1 in a complex.
Title: Osm1 facilitates the transfer of electrons from Erv1 to fumarate in the redox-regulated import pathway in the mitochondrial intermembrane space.
Role of tryptophan residues of Erv1: Trp95 and Trp183 are important for its folding and oxidase function.
Title: Role of tryptophan residues of Erv1: Trp95 and Trp183 are important for its folding and oxidase function.
these results confirm that GSH is critical for cytosolic Fe-S protein biogenesis and iron regulation, whereas ruling out significant roles for Erv1 or Mia40 in these pathways.
Title: Cytosolic Fe-S Cluster Protein Maturation and Iron Regulation Are Independent of the Mitochondrial Erv1/Mia40 Import System.
Mia40 serves as an electron sink in the Mia40-Erv1 protein import pathway.
Title: Mia40 Protein Serves as an Electron Sink in the Mia40-Erv1 Import Pathway.
Taken together, we conclude that both shuttle cysteine residues are required for Erv1 function, and play complementary, but distinct, roles to ensure rapid turnover of active Erv1.
Title: Mitochondrial thiol oxidase Erv1: both shuttle cysteine residues are required for its function with distinct roles.
Mia40 in cooperation with Erv1 promotes the formation of two disulfide bonds in the substrate protein, ensuring the efficiency of oxidative folding in the intermembrane space of mitochondria.
Title: In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin-dependent sulfhydryl oxidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables flavin-dependent sulfhydryl oxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables flavin-dependent sulfhydryl oxidase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables thiol oxidase activity	IEA Inferred from Electronic Annotation more info
enables thiol oxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular iron ion homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in protein import into mitochondrial intermembrane space	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial intermembrane space	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial intermembrane space	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: flavin-linked sulfhydryl oxidase

NP_011543.4

EC 1.8.3.2

Flavin-linked sulfhydryl oxidase of the mitochondrial IMS; N-terminus is an intrinsically disordered domain that in the cytosol helps target Erv1p to mitochondria, and in the intermembrane space oxidizes Mia40p as part of a disulfide relay system that promotes intermembrane space retention of imported proteins; functional ortholog of human GFER (ALR); human GFER carrying N-terminal 21 amino acids of Erv1p functionally complements the lethality of the erv1 null mutation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

543553..544205

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181158.3 → NP_011543.4 TPA: flavin-linked sulfhydryl oxidase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011543.4

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUG4, P27882

UniProtKB/TrEMBL

A6ZV17, B3LIJ1, C8Z8S3, G2WEB9, N1P230

Conserved Domains (1) summary

COG5054
Location:4 → 189

ERV1; Mitochondrial sulfhydryl oxidase involved in the biogenesis of cytosolic Fe/S proteins [Posttranslational modification, protein turnover, chaperones]