PIF1 DNA helicase PIF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PIF1
Official Full Name: DNA helicase PIF1
Primary source: SGD:S000004526
Locus tag: YML061C
See related: AllianceGenome:SGD:S000004526; FungiDB:YML061C; VEuPathDB:YML061C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TST1
Summary: Enables DNA binding activity; DNA helicase activity; and telomerase inhibitor activity. Involved in several processes, including DNA metabolic process; G-quadruplex DNA unwinding; and negative regulation of telomere maintenance via telomerase. Located in mitochondrial membrane; nuclear replication fork; and site of double-strand break. Used to study cancer and progeria. Orthologous to human PIF1 (PIF1 5'-to-3' DNA helicase). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See PIF1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (148953..151532, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Two residues in the DNA binding site of Pif1 helicase are essential for nuclear functions but dispensable for mitochondrial respiratory growth.
Title: Two residues in the DNA binding site of Pif1 helicase are essential for nuclear functions but dispensable for mitochondrial respiratory growth.
Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.
Title: Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.
Identification of the nuclear localization signal in the Saccharomyces cerevisiae Pif1 DNA helicase.
Title: Identification of the nuclear localization signal in the Saccharomyces cerevisiae Pif1 DNA helicase.
Single-molecule visualization of Pif1 helicase translocation on single-stranded DNA.
Title: Single-molecule visualization of Pif1 helicase translocation on single-stranded DNA.
Pif1 family helicases promote mutation avoidance during DNA replication.
Title: Pif1 family helicases promote mutation avoidance during DNA replication.
Rad51-mediated interhomolog recombination during budding yeast meiosis is promoted by the meiotic recombination checkpoint and the conserved Pif1 helicase.
Title: Rad51-mediated interhomolog recombination during budding yeast meiosis is promoted by the meiotic recombination checkpoint and the conserved Pif1 helicase.
Identification of flexible Pif1-DNA interactions and their impacts on enzymatic activities.
Title: Identification of flexible Pif1-DNA interactions and their impacts on enzymatic activities.
Strand switching mechanism of Pif1 helicase induced by its collision with a G-quadruplex embedded in dsDNA.
Title: Strand switching mechanism of Pif1 helicase induced by its collision with a G-quadruplex embedded in dsDNA.
Pif1 Activity is Modulated by DNA Sequence and Structure.
Title: Pif1 Activity is Modulated by DNA Sequence and Structure.
Dynamic regulation of Pif1 acetylation is crucial to the maintenance of genome stability.
Title: Dynamic regulation of Pif1 acetylation is crucial to the maintenance of genome stability.

Submit: New GeneRIF Correction See all GeneRIFs (61)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5'-3' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-3' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables G-quadruplex DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables G-quadruplex DNA binding	IEA Inferred from Electronic Annotation more info
enables centromeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables telomerase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables telomerase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables telomerase inhibitor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in G-quadruplex DNA unwinding	IBA Inferred from Biological aspect of Ancestor more info
involved_in G-quadruplex DNA unwinding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial genome maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial genome maintenance	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial genome maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of telomere maintenance via telomerase	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of telomere maintenance via telomerase	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of telomere maintenance via telomerase	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork reversal	IBA Inferred from Biological aspect of Ancestor more info
involved_in replication fork reversal	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IEA Inferred from Electronic Annotation more info
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in translesion synthesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
is_active_in nuclear replication fork	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA helicase PIF1

NP_013650.1

EC 3.6.4.12

DNA helicase, potent G-quadruplex DNA binder/unwinder; possesses strand annealing activity; promotes DNA synthesis during break-induced replication; involved in crossover recombination; works with Hrq1p to maintain telomere length homeostasis; translation from different start sites produces mitochondrial (DNA repair and recombination) and nuclear (catalytic inhibitor of telomerase) isoforms; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

148953..151532 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182420.1 → NP_013650.1 TPA: DNA helicase PIF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013650.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZB2, P07271

UniProtKB/TrEMBL

C7GVZ7

Conserved Domains (2) summary

COG0507
Location:4 → 763

RecD; ATP-dependent exoDNAse (exonuclease V), alpha subunit, helicase superfamily I [Replication, recombination and repair]

cl28899
Location:237 → 542

DEAD-like_helicase_N; N-terminal helicase domain of the DEAD-box helicase superfamily