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CUE1 Cue1p [ Saccharomyces cerevisiae S288C ]

Gene ID: 855306, updated on 1-Aug-2024

Summary

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Gene symbol
CUE1
Gene description
Cue1p
Primary source
FungiDB:YMR264W
Locus tag
YMR264W
See related
SGD:S000004877; AllianceGenome:SGD:S000004877; VEuPathDB:YMR264W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
KIS4
Summary
Enables ubiquitin binding activity and ubiquitin-protein transferase activator activity. Involved in establishment of protein localization to endoplasmic reticulum membrane and ubiquitin-dependent ERAD pathway. Is integral component of endoplasmic reticulum membrane. Part of Doa10p ubiquitin ligase complex and Hrd1p ubiquitin ligase ERAD-L complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See CUE1 in Genome Data Viewer
Location:
chromosome: XIII
Exon count:
1
Sequence:
Chromosome: XIII; NC_001145.3 (795805..796416)

Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene putative endodeoxyribonuclease Neighboring gene Sap30p Neighboring gene uncharacterized protein Neighboring gene Rsn1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae. Buchanan BW, et al. J Vis Exp, 2016 Apr 18. PMID 27167179, Free PMC Article
  2. A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms. Metzger MB, et al. Mol Cell, 2013 May 23. PMID 23665230, Free PMC Article
  3. A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation. Kostova Z, et al. J Cell Sci, 2009 May 1. PMID 19366730, Free PMC Article
  4. Autoregulation of an E2 enzyme by ubiquitin-chain assembly on its catalytic residue. Ravid T, et al. Nat Cell Biol, 2007 Apr. PMID 17310239
  5. Determinants of RING-E2 fidelity for Hrd1p, a membrane-anchored ubiquitin ligase. Bazirgan OA, et al. J Biol Chem, 2006 Dec 22. PMID 17035235

See all (78) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Cue1 positions Ubc7 and the distal acceptor ubiquitin for rapid polyubiquitination.
    Title: The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation.
  2. Data indicate the crystal structure of the Ubc7p-binding region (U7BR) of Cue1p with Ubc7p.
    Title: A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms.
  3. Data indicate that efficient formation of longer ubiquitin chains depends on the Cue1p CUE domain.
    Title: Ubiquitin binding by a CUE domain regulates ubiquitin chain formation by ERAD E3 ligases.
  4. Data show that a discrete C-terminal Ubc7p binding region of Cue1p is required for ER-associated degradation and for Ubc7p-dependent ubiquitylation by Hrd1p in vitro.
    Title: A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation.
  5. reveal a previously unobserved stimulation of Ubc7p E2 activity by Cue1p
    Title: Cue1p is an activator of Ubc7p E2 activity in vitro and in vivo.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ubiquitin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin binding IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin-protein transferase activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in ERAD pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in establishment of protein localization to endoplasmic reticulum membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of Doa10p ubiquitin ligase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of Hrd1p ubiquitin ligase ERAD-L complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum HDA PubMed 
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion HDA PubMed 

General protein information

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Preferred Names
Cue1p
NP_013991.1
  • Ubiquitin-binding protein; ER membrane protein that recruits and integrates the ubiquitin-conjugating enzyme Ubc7p into ER membrane-bound ubiquitin ligase complexes that function in the ER-associated degradation (ERAD) pathway for misfolded proteins; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination and to promote diubiquitin elongation, facilitating polyubiquitin chain formation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001145.3 Reference assembly

    Range
    795805..796416
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182771.1 → NP_013991.1  TPA: Cue1p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013991.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W090, P38428, Q03507
    UniProtKB/TrEMBL
    A6ZMX9, B3LMF1, C7GR89, C8ZFD0
    Conserved Domains (2) summary
    cd14424
    Location:67 → 103
    CUE_Cue1p_like; CUE domain found in yeast ubiquitin-binding protein CUE1 (Cue1p), CUE4 (Cue4p) and similar proteins
    pfam18499
    Location:151 → 202
    Cue1_U7BR; Ubc7p-binding region of Cue1
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38428.2

Gene LinkOut

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