U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

BRO1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856021, updated on 11-Sep-2024

Summary

Go to the top of the page Help
Official Symbol
BRO1
Primary source
SGD:S000006005
Locus tag
YPL084W
See related
AllianceGenome:SGD:S000006005; FungiDB:YPL084W; VEuPathDB:YPL084W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
ASI6; LPF2; NPI3; VPS31
Summary
Enables deubiquitinase activator activity. Involved in several processes, including ATP export; intralumenal vesicle formation; and proteolysis. Located in endosome. Orthologous to human PTPN23 (protein tyrosine phosphatase non-receptor type 23). [provided by Alliance of Genome Resources, Apr 2022]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
chromosome: XVI
Exon count:
1
Sequence:
Chromosome: XVI; NC_001148.4 (394038..396572)

Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene Elongator subunit ELP3 Neighboring gene Sec16p Neighboring gene tRNA splicing endonuclease subunit SEN54 Neighboring gene DNA-binding ATPase

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Bro1 binds the Vps20 subunit of ESCRT-III and promotes ESCRT-III regulation by Doa4. Buysse D, et al. Traffic, 2022 Feb. PMID 34908216, Free PMC Article
  2. Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus. Wang X, et al. J Virol, 2011 Jun. PMID 21430061, Free PMC Article
  3. Bro1 binding to Snf7 regulates ESCRT-III membrane scission activity in yeast. Wemmer M, et al. J Cell Biol, 2011 Jan 24. PMID 21263029, Free PMC Article
  4. Intervention of Bro1 in pH-responsive Rim20 localization in Saccharomyces cerevisiae. Boysen JH, et al. Eukaryot Cell, 2010 Apr. PMID 20190076, Free PMC Article
  5. Structural basis for endosomal targeting by the Bro1 domain. Kim J, et al. Dev Cell, 2005 Jun. PMID 15935782, Free PMC Article

See all (97) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis.
    Title: Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis.
  2. Novel mutations has been characterized in ESCRT-III Snf7 that trigger activation, and a novel role have been assigned to Bro1, the yeast ALIX ortholog, in Snf7 assembly.
    Title: ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis.
  3. overexpression of Bro1, particularly the V domain, prevented Rfu1 degradation in response to heat shock
    Title: The ESCRT-III adaptor protein Bro1 controls functions of regulator for free ubiquitin chains 1 (Rfu1) in ubiquitin homeostasis.
  4. Bro1 and other Bro1 family members function in parallel with ESCRT-0 to recognize and sort Ub-cargoes
    Title: The yeast Alix homolog Bro1 functions as a ubiquitin receptor for protein sorting into multivesicular endosomes.
  5. Results show that the pH-dependent localization of Rim20 is contingent on the amount of Bro1 in the cell.
    Title: Intervention of Bro1 in pH-responsive Rim20 localization in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables deubiquitinase activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in ATP export IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intralumenal vesicle formation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in intralumenal vesicle formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in late endosome to vacuole transport via multivesicular body sorting pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitochondria-associated ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in multivesicular body sorting pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of protein deubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein deubiquitination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in protein deubiquitination IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in protein localization to endosome IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in vacuolar transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in endosome IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in endosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endosome IEA
Inferred from Electronic Annotation
more info
  
located_in endosome membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in extracellular vesicle IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
Bro1p
NP_015241.1
  • Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates protein sorting and deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes; required for ESCRT-dependent transport of ubiquitinated cargo to the vacuole; localizes to extracellular vesicles during heat conditioning with proposed function in thermotolerance; orthologous to mammalian ESCRT-associated protein ALIX (PDCD6IP)

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001148.4 Reference assembly

    Range
    394038..396572
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183898.1 → NP_015241.1  TPA: Bro1p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_015241.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W3T3, P48582, Q02823
    UniProtKB/TrEMBL
    A6ZWG7, C7GVE5, N1NVH3
    Conserved Domains (2) summary
    cd09237
    Location:368 → 702
    V_ScBro1_like; Protein-interacting V-domain of Saccharomyces cerevisiae Bro1 and related domains
    cd09242
    Location:5 → 347
    BRO1_ScBro1_like; Protein-interacting, N-terminal, Bro1-like domain of Saccharomyces cerevisiae Bro1 and related proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P48582.2

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials
Tools