CIN8 kinesin motor protein CIN8 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CIN8
Gene description: kinesin motor protein CIN8
Primary source: FungiDB:YEL061C
Locus tag: YEL061C
See related: SGD:S000000787; AllianceGenome:SGD:S000000787; VEuPathDB:YEL061C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: KSL2; SDS15
Summary: Enables minus-end-directed microtubule motor activity and plus-end-directed microtubule motor activity. Involved in microtubule depolymerization; regulation of mitotic sister chromatid segregation; and sister chromatid segregation. Located in astral microtubule; kinetochore; and kinetochore microtubule. Used to study breast cancer; colorectal cancer; lung cancer; prostate cancer; and uterine cancer. Human ortholog(s) of this gene implicated in microcephaly with or without chorioretinopathy, lymphedema, or mental retardation. Orthologous to several human genes including KIF11 (kinesin family member 11). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See CIN8 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (36535..39537, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yeast Kinesin-5 Motor Protein CIN8 Promotes Accurate Chromosome Segregation.
Title: Yeast Kinesin-5 Motor Protein CIN8 Promotes Accurate Chromosome Segregation.
Motility of Single Molecules and Clusters of Bi-directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells.
Title: Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells.
Characterization of the motility of monomeric kinesin-5/Cin8.
Title: Characterization of the motility of monomeric kinesin-5/Cin8.
These results suggest that Cin8-PP1 plays a critical role at kinetochores to promote accurate chromosome segregation by controlling Ndc80 attachment to microtubules.
Title: A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation.
Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement
Title: Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement.
Data suggest that approximately four Cin8 motor domains can bind each alpha/beta-tubulin dimer within microtubule lattice; Cin8 binds microtubules not only at the canonical site, but also one or more separate "non-canonical" sites.
Title: The yeast kinesin-5 Cin8 interacts with the microtubule in a noncanonical manner.
The tail domain is one of the factors that regulate Cin8 directionality.
Title: Deletion of the Tail Domain of the Kinesin-5 Cin8 Affects Its Directionality.
Cin8 mutants lacking critical CDK phosphorylation sites suppress the requirement for Cdc14 and Cdc5 in anaphase spindle elongation.
Title: FEAR-mediated activation of Cdc14 is the limiting step for spindle elongation and anaphase progression.
The deletion mutant cells exhibited reduced midzone-directed motility and efficiency to support spindle elongation, indicating the importance of directionality control for the anaphase function of Cin8.
Title: Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry.
Dephosphorylation of Cin8 promotes its binding to the spindle microtubules before the onset of anaphase.
Title: Phospho-regulation of kinesin-5 during anaphase spindle elongation.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IGI Inferred from Genetic Interaction more info	PubMed
enables minus-end-directed microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables minus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed
enables minus-end-directed microtubule motor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables plus-end-directed microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables plus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in initial mitotic spindle pole body separation	IBA Inferred from Biological aspect of Ancestor more info
involved_in initial mitotic spindle pole body separation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in initial mitotic spindle pole body separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule depolymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic sister chromatid segregation	IDA Inferred from Direct Assay more info	PubMed
involved_in spindle elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in spindle organization	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in astral microtubule	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
is_active_in mitotic spindle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle	IEA Inferred from Electronic Annotation more info
is_active_in spindle microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle microtubule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: kinesin motor protein CIN8

NP_010853.2

Bipolar kinesin motor protein; switches from minus- to plus-end-directed motility in vitro depending on conditions; involved in mitotic spindle assembly and chromosome segregation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

36535..39537 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178876.1 → NP_010853.2 TPA: kinesin motor protein CIN8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010853.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLI9, P27895

UniProtKB/TrEMBL

A6ZQP0, B5VH76, C7GXE8, G2WCH3, N1P3E4

Conserved Domains (3) summary

COG1196
Location:493 → 772

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd01364
Location:34 → 486

KISc_BimC_Eg5; Kinesin motor domain, BimC/Eg5 spindle pole proteins

pfam15921
Location:677 → 928

CCDC158; Coiled-coil domain-containing protein 158