PRB1 proteinase B [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PRB1
Gene description: proteinase B
Primary source: FungiDB:YEL060C
Locus tag: YEL060C
See related: SGD:S000000786; AllianceGenome:SGD:S000000786; VEuPathDB:YEL060C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CVT1
Summary: Enables serine-type endopeptidase activity. Involved in several processes, including cellular response to starvation; pexophagy; and protein catabolic process in the vacuole. Predicted to be located in fungal-type vacuole lumen. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in familial hypercholesterolemia and hypobetalipoproteinemia. Orthologous to human PCSK9 (proprotein convertase subtilisin/kexin type 9). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PRB1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (40046..41953, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mutations that eliminate the catalytic activity of Prb1, or the related substrate Prc1, block Saf1 targeting of the zymogen precursor
Title: Prb1 Protease Activity Is Required for Its Recognition by the F-Box Protein Saf1.
Data show that vacuolar protein Prb1 is present in the cellular fraction enriched for the H3 N-terminus endopeptidase activity and this endopeptidase activity is lost in the PRB1 deletion mutant (prb1Delta).
Title: PRB1 is required for clipping of the histone H3 N terminal tail in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables serine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables serine-type peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in pexophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein catabolic process in the vacuole	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in sporulation resulting in formation of a cellular spore	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole	TAS Traceable Author Statement more info	PubMed
located_in fungal-type vacuole lumen	TAS Traceable Author Statement more info	PubMed
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: proteinase B

NP_010854.1

EC 3.4.21.48

Vacuolar proteinase B yscB with H3 N-terminal endopeptidase activity; serine protease of subtilisin family; involved in protein degradation in vacuole; required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via AP-3 pathway; PRB1 has a paralog, YSP3, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

40046..41953 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178875.1 → NP_010854.1 TPA: proteinase B [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010854.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLJ0, P09232

UniProtKB/TrEMBL

G2WCH4

Conserved Domains (2) summary

cd04077
Location:290 → 566

Peptidases_S8_PCSK9_ProteinaseK_like; Peptidase S8 family domain in ProteinaseK-like proteins

pfam05922
Location:182 → 278

Inhibitor_I9; Peptidase inhibitor I9