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A low Smc flux avoids collisions and facilitates chromosome organization in Bacillus subtilis.
Title: A low Smc flux avoids collisions and facilitates chromosome organization in Bacillus subtilis.
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A protein phosphorylation module patterns the Bacillus subtilis spore outer coat.
Title: A protein phosphorylation module patterns the Bacillus subtilis spore outer coat.
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BrfA binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation.BrfA expression is regulated by trans-translation.[BrfA]
Title: Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis.
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Taken together, our study provides a mechanism explaining how FliW and FliS synchronize the production of flagellin with the capacity of the flagellar type III secretion system to secrete flagellin.[FliS, FliW, flagellin]
Title: FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis.
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The authors show that MrfAB function independent of canonical nucleotide excision repair, forming a novel excision repair pathway. They demonstrate that MrfB is a metal-dependent exonuclease and that the N-terminus of MrfB is required for interaction with MrfA.[MrfAB]
Title: A bacterial DNA repair pathway specific to a natural antibiotic.
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SMT revealed that about 30% of SftA molecules move through the cytosol, while a fraction of 70% is septum bound and static. In contrast, only 35% of FtsA molecules are static at midcell, while SpoIIIE molecules diffuse within the membrane and show no enrichment at the septum.[ftsa,spoIIIE,sftA]
Title: Single-Molecule Tracking of DNA Translocases in Bacillus subtilis Reveals Strikingly Different Dynamics of SftA, SpoIIIE, and FtsA.
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Together, these results suggest that DdcA acts by helping to set a threshold of YneA required to establish the cell cycle checkpoint, uncovering a new regulatory step controlling activation of the DNA damage checkpoint in Bacillus subtilis.[DdcA, YneA]
Title: DdcA antagonizes a bacterial DNA damage checkpoint.
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SpoIIIAG assembles into a large and stable 30-fold symmetric complex with a unique mushroom-like architecture/ [SpoIIIAG]
Title: Near-atomic resolution cryoelectron microscopy structure of the 30-fold homooligomeric SpoIIIAG channel essential to spore formation in Bacillus subtilis.
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Here the present a cryo-EM structure of the Bacillus subtilis hibernating 100S ribosome, revealing that the C-terminal domain of LHPF (YvyD) occupies a site on the 30S platform distinct from RMF. Moreover, unlike Escherichia coli RMF, the C-terminal domain of LHPS is directly involved in forming the dimer interface, thereby illustrating the divergent mechanisms by which 100S formation is mediated in the majority of bacter
Title: Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization.
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During sporulation in Bacillus subtilis, the phosphatase SpoIIE is compartmentalized in the small cell by transfer from the polar septum to the adjacent cell pole where SpoIIE is protected from proteolysis and activated.[SpoIIE]
Title: Asymmetric division triggers cell-specific gene expression through coupled capture and stabilization of a phosphatase.