robo2 roundabout, axon guidance receptor, homolog 2 (Drosophila) [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: robo2provided by ZNC
Official Full Name: roundabout, axon guidance receptor, homolog 2 (Drosophila)provided by ZNC
Primary source: ZFIN:ZDB-GENE-001019-1
See related: Ensembl:ENSDARG00000014891 AllianceGenome:ZFIN:ZDB-GENE-001019-1
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: unp1881; sb:eu937
Summary: Predicted to enable identical protein binding activity. Involved in negative regulation of synapse assembly. Acts upstream of or within several processes, including axon development; regulation of extent of cell growth; and sensory perception of touch. Predicted to be located in cell surface. Predicted to be active in neuron projection. Is expressed in several structures, including digestive system; dorsal fin; nervous system; spleen; and trigeminal placode. Human ortholog(s) of this gene implicated in vesicoureteral reflux. Orthologous to human ROBO2 (roundabout guidance receptor 2). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 15

Exon count:: 34

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	15	NC_007126.7 (38372205..39230641)
105	previous assembly	GRCz10 (GCF_000002035.5)	15	NC_007126.6 (38648436..39384041)

Chromosome 15 - NC_007126.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Robo2 Drives Target-Selective Peripheral Nerve Regeneration in Response to Glia-Derived Signals.
Title: Robo2 Drives Target-Selective Peripheral Nerve Regeneration in Response to Glia-Derived Signals.
Genetic loss of roundabout homolog 2 protein (robo2)displayed decreased synaptic oxytocin (OXT) content and robo2 mutants displayed reduced mobility of the actin probe Lifeact-EGFP in OXT synapses.
Title: Robo2 regulates synaptic oxytocin content by affecting actin dynamics.
Genetic analysis showed that Caspase-3, Caspase-9, and p38 MAPK interacted with Slit1a-Robo2 signaling.
Title: Local caspase activation interacts with Slit-Robo signaling to restrict axonal arborization.
The N-terminal domain unique to Robo3a.1 mediates blockade of Robo2 repulsive activity.
Title: Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism.
Robo2-Slit and Dcc-Netrin1 coordinate the axonal projection choices of neurons developing in the forebrain.
Title: Robo2--slit and Dcc--netrin1 coordinate neuron axonal pathfinding within the embryonic axon tracts.
These results reveal that subtype-specific activity of Robo2 regulates subcircuit morphogenesis in the trigeminal sensory system.
Title: Robo2 determines subtype-specific axonal projections of trigeminal sensory neurons.
Robo2 acted initially to split the tract of the postoptic commissure into discrete fascicles upon entering a broad domain of Slit1a expression in the ventrocaudal diencephalon.
Title: Robo-Slit interactions regulate longitudinal axon pathfinding in the embryonic vertebrate brain.
Increased arborization in robo2 mutants was phenocopied by dominant-negative Robo2 expressed in single retinal ganglion cells and rescued by full-length Robo2, indicating that Robo2 acts cell-autonomously.
Title: Slit1a inhibits retinal ganglion cell arborization and synaptogenesis via Robo2-dependent and -independent pathways.
Robo2_tv2 is expressed differentially in most non-neuronal tissues of adult zebrafish whereas robo2_tv1 expression to a great extent is restricted to the brain and eye.
Title: Alternatively spliced Robo2 isoforms in zebrafish and rat.

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General gene information

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Markers

G47888 (e-PCR)
- UniSTS:108655

G39133 (e-PCR)
- UniSTS:28596

G40076 (e-PCR)
- UniSTS:22206

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables identical protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within anterior/posterior axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within anterior/posterior axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axon midline choice point recognition	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within axonal fasciculation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axonogenesis involved in innervation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in brain development	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within central nervous system projection neuron axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within central nervous system projection neuron axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cranial nerve morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in homophilic cell adhesion via plasma membrane adhesion molecules	ISS Inferred from Sequence or Structural Similarity more info
involved_in metanephros development	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of axon extension involved in axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of synapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in olfactory bulb interneuron development	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of axonogenesis	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of collateral sprouting in absence of injury	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within retinal ganglion cell axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within retinal ganglion cell axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinal ganglion cell axon guidance	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within sensory perception of touch	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ureteric bud development	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cell surface	ISS Inferred from Sequence or Structural Similarity more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: roundabout homolog 2

Names: ast; astray; zmp:0000000549

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_131633.1 → NP_571708.1 roundabout homolog 2 precursor

See identical proteins and their annotated locations for NP_571708.1

Status: PROVISIONAL

Source sequence(s)

AF337035

UniProtKB/TrEMBL

Q90Z70

Related

ENSDARP00000012875.8, ENSDART00000014877.9

Conserved Domains (7) summary

cd05726
Location:334 → 423

Ig4_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd07693
Location:29 → 128

Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins

smart00408
Location:438 → 501

IGc2; Immunoglobulin C-2 Type

smart00410
Location:430 → 511

IG_like; Immunoglobulin like

cd00063
Location:529 → 622

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:224 → 312

I-set; Immunoglobulin I-set domain

cl11960
Location:136 → 220

Ig; Immunoglobulin domain

RefSeqs of Annotated Genomes: GCF_000002035.6-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007126.7 Reference GRCz11 Primary Assembly

Range

38372205..39230641

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_021481452.2 → XP_021337127.1 roundabout homolog 2 isoform X1

UniProtKB/TrEMBL

A0A8M9Q0I3

Conserved Domains (6) summary

cd05726
Location:354 → 443

Ig4_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd07693
Location:49 → 148

Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins

smart00409
Location:450 → 531

IG; Immunoglobulin

cd00063
Location:549 → 642

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:244 → 332

I-set; Immunoglobulin I-set domain

cl11960
Location:156 → 240

Ig; Immunoglobulin domain
XM_017351000.3 → XP_017206489.1 roundabout homolog 2 isoform X2

UniProtKB/TrEMBL

A0A8M6YUU1
XM_021481455.2 → XP_021337130.1 roundabout homolog 2 isoform X3

UniProtKB/TrEMBL

A0A8M9QPX8

Conserved Domains (6) summary

cd05726
Location:334 → 423

Ig4_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd07693
Location:29 → 128

Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins

smart00409
Location:430 → 511

IG; Immunoglobulin

cd00063
Location:529 → 622

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:224 → 312

I-set; Immunoglobulin I-set domain

cl11960
Location:136 → 220

Ig; Immunoglobulin domain
XM_021481456.2 → XP_021337131.1 roundabout homolog 2 isoform X4

UniProtKB/TrEMBL

A0A8M9QLH5

Conserved Domains (6) summary

cd05726
Location:330 → 419

Ig4_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd07693
Location:29 → 128

Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins

smart00409
Location:426 → 507

IG; Immunoglobulin

cd00063
Location:525 → 618

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:224 → 312

I-set; Immunoglobulin I-set domain

cl11960
Location:136 → 220

Ig; Immunoglobulin domain