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    DGKG diacylglycerol kinase gamma [ Homo sapiens (human) ]

    Gene ID: 1608, updated on 10-Dec-2024

    Summary

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    Official Symbol
    DGKGprovided by HGNC
    Official Full Name
    diacylglycerol kinase gammaprovided by HGNC
    Primary source
    HGNC:HGNC:2853
    See related
    Ensembl:ENSG00000058866 MIM:601854; AllianceGenome:HGNC:2853
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    DAGK3; DGK-GAMMA
    Summary
    This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
    Expression
    Broad expression in brain (RPKM 8.0), heart (RPKM 3.6) and 15 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See DGKG in Genome Data Viewer
    Location:
    3q27.2-q27.3
    Exon count:
    25
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (186147201..186362234, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (188962845..189177970, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (185864990..186080023, complement)

    Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124909470 Neighboring gene ETS variant transcription factor 5 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr3:185815375-185815888 Neighboring gene ETV5 antisense RNA 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20931 Neighboring gene OCT4-NANOG-H3K4me1 hESC enhancer GRCh37_chr3:185826419-185827397 Neighboring gene uncharacterized LOC105374257 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:185911899-185912696 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:185927717-185928344 Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr3:185933195-185933911 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20932 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:185939275-185940188 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14981 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20933 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20934 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20935 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20936 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20937 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14982 Neighboring gene ReSE screen-validated silencer GRCh37_chr3:185986639-185986811 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:186078791-186079308 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14983 Neighboring gene NANOG hESC enhancer GRCh37_chr3:186100205-186100706 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:186131925-186132644 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14984 Neighboring gene long intergenic non-protein coding RNA 2020 Neighboring gene long intergenic non-protein coding RNA 2052 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:186194185-186194957 Neighboring gene long intergenic non-protein coding RNA 2051

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells. Kai M, et al. J Biol Chem, 1994 Jul 15. PMID 8034597
    2. EF-hand motifs of alpha, beta and gamma isoforms of diacylglycerol kinase bind calcium with different affinities and conformational changes. Yamada K, et al. Biochem J, 1997 Jan 1. PMID 9003401, Free PMC Article
    3. Mapping and genomic characterization of the gene encoding diacylglycerol kinase gamma (DAGK3): assessment of its role in dominant optic atrophy (OPA1). Stöhr H, et al. Hum Genet, 1999 Jan. PMID 10071200
    4. Regulatory role of diacylglycerol kinase gamma in macrophage differentiation of leukemia cells. Yamada K, et al. Biochem Biophys Res Commun, 2003 May 23. PMID 12732202
    5. Diacylglycerol kinase γ predicts prognosis and functions as a tumor suppressor by negatively regulating glucose transporter 1 in hepatocellular carcinoma. Guo Z, et al. Exp Cell Res, 2018 Dec 15. PMID 30399372

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma.
      Title: Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma.
    2. Characterization of alpha-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor.
      Title: Characterization of α-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor.
    3. Decreased expression of DGKgamma in hepatocellular carcinoma tissues was an independent risk factor for a poor prognosis.
      Title: Diacylglycerol kinase γ predicts prognosis and functions as a tumor suppressor by negatively regulating glucose transporter 1 in hepatocellular carcinoma.
    4. Epigenetic silencing of DGKG expression is associated with skin cancer.
      Title: Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer.
    5. these results indicate that DGKG is an anionic phospholipid binding protein that preferably interacts with a small highly charged head group that is very close to the glycerol or sphingosine backbone.
      Title: Diacylglycerol kinase γ is a novel anionic phospholipid binding protein with a selective binding preference.
    6. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
      Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
    7. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
      Title: Implication of genetic variants near NEGR1, SEC16B, TMEM18, ETV5/DGKG, GNPDA2, LIN7C/BDNF, MTCH2, BCDIN3D/FAIM2, SH2B1, FTO, MC4R, and KCTD15 with obesity and type 2 diabetes in 7705 Chinese.
    8. Observational study of gene-disease association. (HuGE Navigator)
      Title: Analyses of shared genetic factors between asthma and obesity in children.
    9. the interaction of diacylglycerol kinase gamma with the Src homology 2 and C1 domains of beta2-chimaerin is induced synergistically by Phorbol ester and hydrogen peroxide
      Title: Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerol kinase gamma with the Src homology 2 and C1 domains of beta2-chimaerin.
    10. diacylglycerol kinase gamma regulates macrophage differentiation.
      Title: Regulatory role of diacylglycerol kinase gamma in macrophage differentiation of leukemia cells.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    EBI GWAS Catalog

    Description
    Genome-wide association yields new sequence variants at seven loci that associate with measures of obesity.
    EBI GWAS Catalog

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Potential readthrough

    Included gene: ETV5

    Homology

    Clone Names

    • MGC104993, MGC133330

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent diacylglycerol kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP-dependent diacylglycerol kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP-dependent diacylglycerol kinase activity TAS
    Traceable Author Statement
    more info
    		  
    enables calcium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lipid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in diacylglycerol metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in diacylglycerol metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in glycerolipid metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in intracellular signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in lipid phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of phospholipase C/protein kinase C signal transduction ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in neuron development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in phosphatidic acid biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in phosphatidic acid biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in phospholipase C-activating G protein-coupled receptor signaling pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in platelet activation TAS
    Traceable Author Statement
    more info
    		  
    involved_in regulation of dendrite development IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
    		  

    General protein information

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    Preferred Names
    diacylglycerol kinase gamma
    Names
    DAG kinase gamma
    diacylglycerol kinase, gamma 90kDa
    diacylglyerol kinase gamma
    diglyceride kinase gamma
    NP_001074213.1
    NP_001074214.1
    NP_001337.2

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001080744.2 → NP_001074213.1  diacylglycerol kinase gamma isoform 2

      See identical proteins and their annotated locations for NP_001074213.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 2) that is shorter than isoform 1.
      Source sequence(s)
      AC112649, AC117414, BC089411, BM669549
      Consensus CDS
      CCDS43182.1
      UniProtKB/TrEMBL
      Q2M589
      Related
      ENSP00000339777.4, ENST00000344484.8
      Conserved Domains (7) summary
      smart00109
      Location:336 → 377
      C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)
      smart00045
      Location:553 → 727
      DAGKa; Diacylglycerol kinase accessory domain (presumed)
      smart00046
      Location:436 → 532
      DAGKc; Diacylglycerol kinase catalytic domain (presumed)
      cd00029
      Location:272 → 321
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd00051
      Location:179 → 248
      EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
      pfam13499
      Location:180 → 244
      EF-hand_7; EF-hand domain pair
      pfam14513
      Location:5 → 175
      DAG_kinase_N; Diacylglycerol kinase N-terminus

    2. NM_001080745.2 → NP_001074214.1  diacylglycerol kinase gamma isoform 3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 3) that is shorter than isoform 1.
      Source sequence(s)
      AC112649, AC117414, BC089411, BC112363, BM669549
      Consensus CDS
      CCDS43181.1
      UniProtKB/TrEMBL
      Q2M589
      Related
      ENSP00000371599.4, ENST00000382164.8
      Conserved Domains (6) summary
      smart00045
      Location:539 → 713
      DAGKa; Diacylglycerol kinase accessory domain (presumed)
      smart00046
      Location:397 → 518
      DAGKc; Diacylglycerol kinase catalytic domain (presumed)
      cd00029
      Location:272 → 321
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd00051
      Location:179 → 248
      EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
      pfam13499
      Location:180 → 244
      EF-hand_7; EF-hand domain pair
      pfam14513
      Location:5 → 175
      DAG_kinase_N; Diacylglycerol kinase N-terminus

    3. NM_001346.3 → NP_001337.2  diacylglycerol kinase gamma isoform 1

      See identical proteins and their annotated locations for NP_001337.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      AC112649, AC117414, BC089411, BM669549, D26135
      Consensus CDS
      CCDS3274.1
      UniProtKB/Swiss-Prot
      B2RAH4, P49619, Q2M1H4, Q5FWG1
      UniProtKB/TrEMBL
      Q2M589
      Related
      ENSP00000265022.3, ENST00000265022.8
      Conserved Domains (7) summary
      smart00109
      Location:336 → 377
      C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)
      smart00045
      Location:578 → 752
      DAGKa; Diacylglycerol kinase accessory domain (presumed)
      smart00046
      Location:436 → 557
      DAGKc; Diacylglycerol kinase catalytic domain (presumed)
      cd00029
      Location:272 → 321
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd00051
      Location:179 → 248
      EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
      pfam13499
      Location:180 → 244
      EF-hand_7; EF-hand domain pair
      pfam14513
      Location:5 → 175
      DAG_kinase_N; Diacylglycerol kinase N-terminus

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

      Range
      186147201..186362234 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060927.1 Alternate T2T-CHM13v2.0

      Range
      188962845..189177970 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P49619.3 GenPept UniProtKB/Swiss-Prot:P49619

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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