TPST2 tyrosylprotein sulfotransferase 2 [Homo sapiens (human)] - Gene

Summary

Official Symbol: TPST2provided by HGNC
Official Full Name: tyrosylprotein sulfotransferase 2provided by HGNC
Primary source: HGNC:HGNC:12021
See related: Ensembl:ENSG00000128294 MIM:603126; AllianceGenome:HGNC:12021
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: TPST-2; TANGO13B
Summary: The protein encoded by this gene catalyzes the O-sulfation of tyrosine residues within acidic regions of proteins. The encoded protein is a type II integral membrane protein found in the Golgi body. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
Expression: Ubiquitous expression in pancreas (RPKM 24.6), placenta (RPKM 15.2) and 24 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 22q12.1

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	22	NC_000022.11 (26521996..26590132, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	22	NC_060946.1 (26984404..27052464, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	22	NC_000022.10 (26917962..26986096, complement)

Chromosome 22 - NC_000022.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition.
Title: Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition.
Genetic variations in the host dependency factors ALCAM and TPST2 impact HIV-1 disease progression.
Title: Genetic variations in the host dependency factors ALCAM and TPST2 impact HIV-1 disease progression.
Using a quantum mechanics protocol of alanine scanning, the study identified unequivocally the role of the amino acids involved in the TPST-2 catalytic mechanism.
Title: Computational Evidence for the Catalytic Mechanism of Tyrosylprotein Sulfotransferases: A Density Functional Theory Investigation.
the first crystal structure of the human tyrosylprotein sulfotransferase isoform 2 complexed with a substrate peptide (C4P5Y3) derived from complement C4 and 3'-phosphoadenosine-5'-phosphate at 1.9 A resolution, is reported.
Title: Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction.
The p.R153H variant was found in a family with hereditary pancreatitis; however, it did not segregate with the disease.
Title: Sequence analysis of the human tyrosylprotein sulfotransferase-2 gene in subjects with chronic pancreatitis.
Observational study of gene-disease association. (HuGE Navigator)
Title: Sequence analysis of the human tyrosylprotein sulfotransferase-2 gene in subjects with chronic pancreatitis.
The kinetic parameters of tyrosylprotein sulfotransferase-1 and -2, catalyzing tyrosine sulfation of CCR8 peptides, were determined using liquid chromatography electrospray ionisation mass spectrometry.
Title: Mass spectrometric kinetic analysis of human tyrosylprotein sulfotransferase-1 and -2.
Tyrosine sulfation of CCR5 N-terminal peptide follows a discrete pattern and temporal sequence
Title: Tyrosine sulfation of CCR5 N-terminal peptide by tyrosylprotein sulfotransferases 1 and 2 follows a discrete pattern and temporal sequence.
Shear stress-dependent upregulation of TPST2 in human endothelium is mediated by a tyrosine kinase-dependent pathway
Title: Shear stress mediates tyrosylprotein sulfotransferase isoform shift in human endothelial cells.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	HIV-1 gp120 is sulfated at positions Y173 and Y177 in the V2 domain by tyrosyl sulfotransferase 2 (TPST2), leading to modulate gp120 epitope exposure and neutralization sensitivity	PubMed
	env	Sulfation of CCR5 by tyrosylprotein sulfotranferase-2 is crucial for mediating interaction with HIV-1 gp120. The pattern and the rate of sulfation for CCR5 depend on the number of amino acids N-terminal of Tyr-3	PubMed
	env	The chemokine receptor CCR5 is posttranslationally modified by sulfation of its N-terminal tyrosines; sulfated tyrosines contribute to the binding of CCR5 to MIP-1 alpha, MIP-1 beta, and HIV-1 gp120/CD4 complexes and to the ability of HIV-1 to enter cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

WI-19497 (e-PCR)
- UniSTS:26232

SHGC-37519 (e-PCR)
- UniSTS:4266

G49193 (e-PCR)
- UniSTS:108972

G49244 (e-PCR)
- UniSTS:109000

WI-14070 (e-PCR)
- UniSTS:14847

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables protein-tyrosine sulfotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein-tyrosine sulfotransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-tyrosine sulfotransferase activity	TAS Traceable Author Statement more info

Process	Evidence Code	Pubs
involved_in 3'-phosphoadenosine 5'-phosphosulfate metabolic process	TAS Traceable Author Statement more info
involved_in peptidyl-tyrosine sulfation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi membrane	TAS Traceable Author Statement more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info

General protein information

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Preferred Names: protein-tyrosine sulfotransferase 2

Names: transport and golgi organization 13 homolog B; tyrosylprotein phosphotransferase 2

NP_001008566.1

EC 2.8.2.20

NP_001349851.1

EC 2.8.2.20

NP_001349852.1

EC 2.8.2.20

NP_003586.3

EC 2.8.2.20

XP_024308062.1

EC 2.8.2.20

XP_047297505.1

EC 2.8.2.20

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001008566.3 → NP_001008566.1 protein-tyrosine sulfotransferase 2 isoform 1

See identical proteins and their annotated locations for NP_001008566.1

Status: REVIEWED

Source sequence(s)

Z95115

Consensus CDS

CCDS13839.1

UniProtKB/Swiss-Prot

B3KQA7, O60704, Q6FI98, Q9H0V4

UniProtKB/TrEMBL

B7Z976

Related

ENSP00000381180.2, ENST00000398110.6

Conserved Domains (1) summary

pfam13469
Location:71 → 262

Sulfotransfer_3; Sulfotransferase family
NM_001362922.2 → NP_001349851.1 protein-tyrosine sulfotransferase 2 isoform 1

Status: REVIEWED

Source sequence(s)

Z95115

Consensus CDS

CCDS13839.1

UniProtKB/Swiss-Prot

B3KQA7, O60704, Q6FI98, Q9H0V4

UniProtKB/TrEMBL

B7Z976

Conserved Domains (1) summary

pfam13469
Location:71 → 262

Sulfotransfer_3; Sulfotransferase family
NM_001362923.2 → NP_001349852.1 protein-tyrosine sulfotransferase 2 isoform 2

Status: REVIEWED

Source sequence(s)

Z95115

Conserved Domains (1) summary

pfam13469
Location:125 → 316

Sulfotransfer_3; Sulfotransferase family
NM_003595.5 → NP_003586.3 protein-tyrosine sulfotransferase 2 isoform 1

See identical proteins and their annotated locations for NP_003586.3

Status: REVIEWED

Source sequence(s)

Z95115

Consensus CDS

CCDS13839.1

UniProtKB/Swiss-Prot

B3KQA7, O60704, Q6FI98, Q9H0V4

UniProtKB/TrEMBL

B7Z976

Related

ENSP00000339813.4, ENST00000338754.9

Conserved Domains (1) summary

pfam13469
Location:71 → 262

Sulfotransfer_3; Sulfotransferase family

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000022.11 Reference GRCh38.p14 Primary Assembly

Range

26521996..26590132 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047441549.1 → XP_047297505.1 protein-tyrosine sulfotransferase 2 isoform X2
XM_024452294.2 → XP_024308062.1 protein-tyrosine sulfotransferase 2 isoform X1

Conserved Domains (1) summary

pfam13469
Location:141 → 332

Sulfotransfer_3; Sulfotransferase family