Car4 carbonic anhydrase 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Car4provided by MGI
Official Full Name: carbonic anhydrase 4provided by MGI
Primary source: MGI:MGI:1096574
See related: Ensembl:ENSMUSG00000000805 AllianceGenome:MGI:1096574
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ca4
Summary: Enables carbonate dehydratase activity. Predicted to be involved in bicarbonate transport and one-carbon metabolic process. Predicted to act upstream of or within carbon dioxide transport and regulation of pH. Located in plasma membrane. Is expressed in several structures, including cardiovascular system; genitourinary system; lung; nervous system; and taste bud. Human ortholog(s) of this gene implicated in retinitis pigmentosa. Orthologous to human CA4 (carbonic anhydrase 4). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Biased expression in colon adult (RPKM 148.4), placenta adult (RPKM 86.4) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 C; 11 51.34 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (84848580..84856880)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (84957754..84966054)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors.
Title: Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors.
Mice with a mutant thyroid hormone receptor alpha1 have permanently reduced Car4 in lung and brain. These findings identify a previously unknown marker of thyroid hormone receptor alpha1 action in brain and lung.
Title: Thyroid hormone drives the expression of mouse carbonic anhydrase Car4 in kidney, lung and brain.
two CA isoforms, CAII and CAIV, are distributed along the epididymal epithelium and appear with the onset of puberty
Title: Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.
eosinophils express CAR4 following IL-5 or allergen exposure, and that CAR4 is involved in regulating the lung transcriptome associated with allergic airway inflammation
Title: Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils.
membrane-associated CA IV contributes robust catalytic activity intracellularly, and this activity participates in regulating H(+) dynamics in the cytosol in neurons
Title: GPI-anchored carbonic anhydrase IV displays both intra- and extracellular activity in cRNA-injected oocytes and in mouse neurons.
Data show a 32.13% reduction in total CA activity in CA IV(-/-) mice.
Title: Role of carbonic anhydrase IV in the bicarbonate-mediated activation of murine and human sperm.
Car4, Car6, and Car12 showed moderate expression levels in the mouse harderian gland.
Title: Carbonic anhydrases in the mouse harderian gland.
Both methazolamide and dichlorphenamide increased the tetanic muscle force in the treated M. extensor digitorum longus muscle group.
Title: Evaluation of the therapeutic potential of carbonic anhydrase inhibitors in two animal models of dystrophin deficient muscular dystrophy.
study demonstrated that the sour-sensing cells act as the taste sensors for carbonation, and showed that carbonic anhydrase 4, a glycosylphosphatidylinositol-anchored enzyme, functions as the principal CO2 taste sensor
Title: The taste of carbonation.
Decreased expression of colonic Slc26a3 and carbonic anhydrase iv as a cause of fatal infectious diarrhea in mice.
Title: Decreased expression of colonic Slc26a3 and carbonic anhydrase iv as a cause of fatal infectious diarrhea in mice.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Targeted (5) 1 citation

General gene information

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Markers

U37091 (e-PCR)
- UniSTS:125738

AW456718 (e-PCR)
- UniSTS:180273

U37091 (e-PCR)
- UniSTS:180274

Car4 (e-PCR), detects polymorphism
- UniSTS:525883

Car4 (e-PCR), detects polymorphism
- UniSTS:506773

Car4 (e-PCR), detects polymorphism
- UniSTS:502750

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carbonate dehydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables carbonate dehydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables carbonate dehydratase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in bicarbonate transport	IEA Inferred from Electronic Annotation more info
involved_in bicarbonate transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within carbon dioxide transport	IC Inferred by Curator more info	PubMed
involved_in one-carbon metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of pH	IC Inferred by Curator more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in brush border membrane	IEA Inferred from Electronic Annotation more info
located_in brush border membrane	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum-Golgi intermediate compartment	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum-Golgi intermediate compartment	ISO Inferred from Sequence Orthology more info
located_in external side of plasma membrane	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in extracellular exosome	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in rough endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in rough endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in sarcolemma	ISO Inferred from Sequence Orthology more info
located_in sarcoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in secretory granule membrane	IEA Inferred from Electronic Annotation more info
located_in secretory granule membrane	ISO Inferred from Sequence Orthology more info
located_in trans-Golgi network	IEA Inferred from Electronic Annotation more info
located_in trans-Golgi network	ISO Inferred from Sequence Orthology more info
located_in transport vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in transport vesicle membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: carbonic anhydrase 4

Names: CA-IV; carbonate dehydratase IV; carbonic anhydrase IV

NP_031633.1

EC 4.2.1.1

XP_006532158.1

EC 4.2.1.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001430872.1 → NP_001417801.1 carbonic anhydrase 4 isoform 2

Status: VALIDATED

Source sequence(s)

AL669859
NM_007607.3 → NP_031633.1 carbonic anhydrase 4 isoform 1 precursor

See identical proteins and their annotated locations for NP_031633.1

Status: VALIDATED

Source sequence(s)

AL669859

Consensus CDS

CCDS25190.1

UniProtKB/Swiss-Prot

Q64444

UniProtKB/TrEMBL

Q3U3I1

Related

ENSMUSP00000099483.4, ENSMUST00000103194.10

Conserved Domains (1) summary

cd03117
Location:48 → 276

alpha_CA_IV_XV_like; Carbonic anhydrase alpha, CA_IV, CA_XV, like isozymes. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

84848580..84856880

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006532095.5 → XP_006532158.1 carbonic anhydrase 4 isoform X1

Conserved Domains (1) summary

cl00012
Location:1 → 186

alpha_CA; Carbonic anhydrase alpha (vertebrate-like) group. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon ...